Tracking heavy water (D 2 O) incorporation for identifying and sorting active microbial cells

Berry, David; Mader, Esther; Lee, Tae Kwon; Woebken, Dagmar; Wang, Yun; Zhu, Di; Palatinszky, Marton; Schintlmeister, Arno; Schmid, Markus C.; Hanson, Buck T.; Shterzer, Naama; Mizrahi, Itzhak; Rauch, Isabella; Decker, Thomas; Bocklitz, Thomas; Popp, Jürgen; Gibson, Christopher M.; Fowler, Patrick W.; Huang, Wei E.; Wagner, Michael

doi:10.1073/pnas.1420406112

Tracking heavy water (D ₂ O) incorporation for identifying and sorting active microbial cells

Journal Article · Mon Dec 29 23:00:00 EST 2014 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1420406112· OSTI ID:1235155

Berry, David ^[1]; Mader, Esther ^[1]; Lee, Tae Kwon ^[1]; Woebken, Dagmar ^[1]; Wang, Yun ^[2]; Zhu, Di ^[3]; Palatinszky, Marton ^[1]; Schintlmeister, Arno ^[4]; Schmid, Markus C. ^[1]; Hanson, Buck T. ^[1]; Shterzer, Naama ^[5]; Mizrahi, Itzhak ^[5]; Rauch, Isabella ^[6]; Decker, Thomas ^[6]; Bocklitz, Thomas ^[7]; Popp, Jürgen ^[8]; Gibson, Christopher M. ^[9]; Fowler, Patrick W. ^[9]; Huang, Wei E. ^[10]; Wagner, Michael ^[4]

Division of Microbial Ecology, Department of Microbiology and Ecosystem Science and
Single-Cell Center, Chinese Academy of Sciences Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China,, Kroto Research Institute and
Kroto Research Institute and
Division of Microbial Ecology, Department of Microbiology and Ecosystem Science and, Large Instrument Facility for Advanced Isotope Research, Faculty of Life Sciences, University of Vienna, A-1090 Vienna, Austria,
Department of Ruminant Sciences, Institute of Animal Science, Agricultural Research Organization, Bet-Dagan 50250, Israel,
Department of Microbiology, Immunobiology, and Genetics, Max F. Perutz Laboratories, University of Vienna, A-1030 Vienna, Austria,
Institute of Physical Chemistry and Abbe Center of Photonics, University of Jena, D-07743 Jena, Germany,
Institute of Physical Chemistry and Abbe Center of Photonics, University of Jena, D-07743 Jena, Germany,, Institute of Photonic Technology, D-07745 Jena, Germany, and
Department of Chemistry, University of Sheffield, Sheffield S3 7HQ, United Kingdom,
Kroto Research Institute and, Department of Engineering Science, University of Oxford, Oxford OX1 3PJ, United Kingdom

Significance

Measuring activity patterns of microbes in their natural environment is essential for understanding ecosystems and the multifaceted interactions of microorganisms with eukaryotes. In this study, we developed a technique that allows fast and nondestructive activity measurements of microbial communities on a single-cell level. Microbial communities were amended with heavy water (D ₂ O), a treatment that does not change the available substrate pool. After incubation, physiologically active cells are rapidly identified with Raman microspectroscopy by measuring cellular D incorporation. Using this approach, we characterized the activity patterns of two dominant microbes in mouse cecum samples amended with different carbohydrates and discovered previously unidentified bacteria stimulated by mucin and/or glucosamine by combining Raman microspectroscopy and optical tweezer-based sorting.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1235155

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 2 Vol. 112; ISSN 0027-8424

Publisher:: Proceedings of the National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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