Tn-seq of Caulobacter crescentus under uranium stress reveals genes essential for detoxification and stress tolerance

Yung, Mimi C.; Park, Dan M.; Overton, K. Wesley; Blow, Matthew J.; Hoover, Cindi A.; Smit, John R.; Murray, Sean R.; Ricci, Dante P.; Christen, Beat; Bowman, Grant R.; Jiao, Yongqin

doi:10.1128/JB.00382-15

Tn-seq of Caulobacter crescentus under uranium stress reveals genes essential for detoxification and stress tolerance

Journal Article · Mon Jul 20 00:00:00 EDT 2015 · Journal of Bacteriology

DOI:https://doi.org/10.1128/JB.00382-15· OSTI ID:1234593

Yung, Mimi C. ^[1]; Park, Dan M. ^[1]; Overton, K. Wesley ^[1]; Blow, Matthew J. ^[2]; Hoover, Cindi A. ^[2]; Smit, John R. ^[3]; Murray, Sean R. ^[4]; Ricci, Dante P. ^[5]; Christen, Beat ^[6]; Bowman, Grant R. ^[7]; Jiao, Yongqin ^[1]

Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
DOE Joint Genome Inst., Walnut Creek, CA (United States)
Univ. of British Columbia, Vancouver, BC (Canada)
California State Univ., Northridge, CA (United States)
Stanford Univ. School of Medicine, Stanford, CA (United States)
ETH Zurich (Switzerland)
Univ. of Wyoming, Laramie, WY (United States)

Ubiquitous aquatic bacterium Caulobacter crescentus is highly resistant to uranium (U) and facilitates U biomineralization and thus holds promise as an agent of U bioremediation. In order to gain an understanding of how C. crescentus tolerates U, we employed transposon (Tn) mutagenesis paired with deep sequencing (Tn-seq) in a global screen for genomic elements required for U resistance. Of the 3,879 annotated genes in the C. crescentus genome, 37 were found to be specifically associated with fitness under U stress, 15 of which were subsequently tested through mutational analysis. Systematic deletion analysis revealed that mutants lacking outer membrane transporters (rsaFa and rsaFb), a stress-responsive transcription factor (cztR), or a ppGpp synthetase/hydrolase (spoT) exhibited a significantly lower survival rate under U stress. RsaFa and RsaFb, which are homologues of TolC in Escherichia coli, have previously been shown to mediate S-layer export. Transcriptional analysis revealed upregulation of rsaF_a and rsaF_b by 4- and 10-fold, respectively, in the presence of U. We additionally show that rsaFa mutants accumulated higher levels of U than the wild type, with no significant increase in oxidative stress levels. These results suggest a function for RsaF_a and RsaF_b in U efflux and/or maintenance of membrane integrity during U stress. In addition, we present data implicating CztR and SpoT in resistance to U stress. Together, our findings reveal novel gene targets that are key to understanding the molecular mechanisms of U resistance in C. crescentus.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC52-07NA27344

OSTI ID:: 1234593

Report Number(s):: LLNL-JRNL--670205

Journal Information:: Journal of Bacteriology, Journal Name: Journal of Bacteriology Journal Issue: 19 Vol. 197; ISSN 0021-9193

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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