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Title: Karyotyping human chromosomes by optical and x-ray ptychography methods

Abstract

Sorting and identifying chromosomes, a process known as karyotyping, is widely used to detect changes in chromosome shapes and gene positions. In a karyotype the chromosomes are identified by their size and therefore this process can be performed by measuring macroscopic structural variables. Chromosomes contain a specific number of basepairs that linearly correlate with their size; therefore, it is possible to perform a karyotype on chromosomes using their mass as an identifying factor. Here, we obtain the first images, to our knowledge, of chromosomes using the novel imaging method of ptychography. We can use the images to measure the mass of chromosomes and perform a partial karyotype from the results. Lastly, we also obtain high spatial resolution using this technique with synchrotron source x-rays.

Authors:
 [1];  [1];  [1];  [1];  [2];  [3];  [1];  [1];  [1];  [1]
  1. Univ. College London, (United Kingdom); Rutherford Appleton Lab., Didcot (United Kingdom)
  2. Univ. College London, (United Kingdom)
  3. Argonne National Lab. (ANL), Argonne, IL (United States)
Publication Date:
Research Org.:
Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source.; Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1233919
Alternate Identifier(s):
OSTI ID: 1196335; OSTI ID: 1249549
Grant/Contract Number:  
AC02-06CH11357
Resource Type:
Journal Article: Published Article
Journal Name:
Biophysical Journal
Additional Journal Information:
Journal Volume: 108; Journal Issue: 3; Journal ID: ISSN 0006-3495
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Shemilt, Laura, Verbanis, Ephanielle, Schwenke, Joerg, Estandarte, Ana  K., Xiong, Gang, Harder, Ross, Parmar, Neha, Yusuf, Mohammed, Zhang, Fucai, and Robinson, Ian  K. Karyotyping human chromosomes by optical and x-ray ptychography methods. United States: N. p., 2015. Web. doi:10.1016/j.bpj.2014.11.3456.
Shemilt, Laura, Verbanis, Ephanielle, Schwenke, Joerg, Estandarte, Ana  K., Xiong, Gang, Harder, Ross, Parmar, Neha, Yusuf, Mohammed, Zhang, Fucai, & Robinson, Ian  K. Karyotyping human chromosomes by optical and x-ray ptychography methods. United States. doi:10.1016/j.bpj.2014.11.3456.
Shemilt, Laura, Verbanis, Ephanielle, Schwenke, Joerg, Estandarte, Ana  K., Xiong, Gang, Harder, Ross, Parmar, Neha, Yusuf, Mohammed, Zhang, Fucai, and Robinson, Ian  K. Sun . "Karyotyping human chromosomes by optical and x-ray ptychography methods". United States. doi:10.1016/j.bpj.2014.11.3456.
@article{osti_1233919,
title = {Karyotyping human chromosomes by optical and x-ray ptychography methods},
author = {Shemilt, Laura and Verbanis, Ephanielle and Schwenke, Joerg and Estandarte, Ana  K. and Xiong, Gang and Harder, Ross and Parmar, Neha and Yusuf, Mohammed and Zhang, Fucai and Robinson, Ian  K.},
abstractNote = {Sorting and identifying chromosomes, a process known as karyotyping, is widely used to detect changes in chromosome shapes and gene positions. In a karyotype the chromosomes are identified by their size and therefore this process can be performed by measuring macroscopic structural variables. Chromosomes contain a specific number of basepairs that linearly correlate with their size; therefore, it is possible to perform a karyotype on chromosomes using their mass as an identifying factor. Here, we obtain the first images, to our knowledge, of chromosomes using the novel imaging method of ptychography. We can use the images to measure the mass of chromosomes and perform a partial karyotype from the results. Lastly, we also obtain high spatial resolution using this technique with synchrotron source x-rays.},
doi = {10.1016/j.bpj.2014.11.3456},
journal = {Biophysical Journal},
number = 3,
volume = 108,
place = {United States},
year = {Sun Feb 01 00:00:00 EST 2015},
month = {Sun Feb 01 00:00:00 EST 2015}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.1016/j.bpj.2014.11.3456

Citation Metrics:
Cited by: 10 works
Citation information provided by
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