Structure of a Rhamnogalacturonan Fragment from Apple Pectin: Implications for Pectin Architecture

Wu, Xiangmei; Mort, Andrew

doi:10.1155/2014/347381

Structure of a Rhamnogalacturonan Fragment from Apple Pectin: Implications for Pectin Architecture

Journal Article · Sat Dec 13 23:00:00 EST 2014 · International Journal of Carbohydrate Chemistry

DOI:https://doi.org/10.1155/2014/347381· OSTI ID:1227787

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Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078-3035, USA

A commercial apple pectin was sequentially digested with the cloned enzymes endopolygalacturonase, galactanase, arabinofuranosidase, xylogalacturonase, and rhamnogalacturonan hydrolase. The rhamnogalacturonan hydrolase-generated oligosaccharides were separated by ultrafiltration, anion exchange, and size-exclusion chromatography. Fractions from the ion exchange chromatography were pooled, lyophilized, and screened by MALDI-TOF MS. An oligosaccharide (RGP14P3) was identified and its structure, α -D-Gal p A- $(1 \to 2)$ - α -L-Rha p - $(1 \to 4)$ - α -D-Gal p A- $(1 \to 2)$ - α -L-Rha p - $(1 \to 4)$ - α -D-Gal p A, determined by 1D and 2D NMR spectrometry. This oligosaccharide probably represents a direct connection between homogalacturonan and rhamnogalacturonan in pectin. Alternatively, it could indicate that the nonreducing end of rhamnogalacturonan starts with a galacturonic acid residue.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: FG02-96ER20215

OSTI ID:: 1227787

Journal Information:: International Journal of Carbohydrate Chemistry, Journal Name: International Journal of Carbohydrate Chemistry Vol. 2014; ISSN 1687-9341

Publisher:: Hindawi Publishing CorporationCopyright Statement

Country of Publication:: Country unknown/Code not available

Language:: English

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