Structure of a Rhamnogalacturonan Fragment from Apple Pectin: Implications for Pectin Architecture
- Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078-3035, USA
A commercial apple pectin was sequentially digested with the cloned enzymes endopolygalacturonase, galactanase, arabinofuranosidase, xylogalacturonase, and rhamnogalacturonan hydrolase. The rhamnogalacturonan hydrolase-generated oligosaccharides were separated by ultrafiltration, anion exchange, and size-exclusion chromatography. Fractions from the ion exchange chromatography were pooled, lyophilized, and screened by MALDI-TOF MS. An oligosaccharide (RGP14P3) was identified and its structure, α -D-Gal p A- - α -L-Rha p - - α -D-Gal p A- - α -L-Rha p - - α -D-Gal p A, determined by 1D and 2D NMR spectrometry. This oligosaccharide probably represents a direct connection between homogalacturonan and rhamnogalacturonan in pectin. Alternatively, it could indicate that the nonreducing end of rhamnogalacturonan starts with a galacturonic acid residue.
- Sponsoring Organization:
- USDOE
- Grant/Contract Number:
- FG02-96ER20215
- OSTI ID:
- 1227787
- Journal Information:
- International Journal of Carbohydrate Chemistry, Journal Name: International Journal of Carbohydrate Chemistry Vol. 2014; ISSN 1687-9341
- Publisher:
- Hindawi Publishing CorporationCopyright Statement
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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