Morphological transformations in the magnetite biomineralizing protein Mms6 in iron solutions: A small-angle x-ray scattering study

Zhang, Honghu; Liu, Xunpei; Feng, Shuren; Wang, Wenjie; Schmidt-Rohr, Klaus; Akinc, Mufit; Nilsen-Hamilton, Marit; Vaknin, David; Mallapragada, Surya

doi:10.1021/la5044377

Morphological transformations in the magnetite biomineralizing protein Mms6 in iron solutions: A small-angle x-ray scattering study

Journal Article · Tue Feb 10 00:00:00 EST 2015 · Langmuir

DOI:https://doi.org/10.1021/la5044377· OSTI ID:1227436

Zhang, Honghu ^[1]; Liu, Xunpei ^[1]; Feng, Shuren ^[1]; Wang, Wenjie ^[2]; Schmidt-Rohr, Klaus ^[1]; Akinc, Mufit ^[1]; Nilsen-Hamilton, Marit ^[1]; Vaknin, David ^[1]; Mallapragada, Surya ^[1]

Ames Lab., Ames, IA (United States); Iowa State Univ., Ames, IA (United States)
Ames Lab., Ames, IA (United States)

In this study, magnetotactic bacteria that produce magnetic nanocrystals of uniform size and well-defined morphologies have inspired the use of biomineralization protein Mms6 to promote formation of uniform magnetic nanocrystals in vitro. Small angle X-ray scattering (SAXS) studies in physiological solutions reveal that Mms6 forms compact globular three-dimensional (3D) micelles (approximately 10 nm in diameter) that are, to a large extent, independent of concentration. In the presence of iron ions in the solutions, the general micellar morphology is preserved, however, with associations among micelles that are induced by iron ions. Compared with Mms6, the m2Mms6 mutant (with the sequence of hydroxyl/carboxyl containing residues in the C-terminal domain shuffled) exhibits subtle morphological changes in the presence of iron ions in solutions. The analysis of the SAXS data is consistent with a hierarchical core–corona micellar structure similar to that found in amphiphilic polymers. The addition of ferric and ferrous iron ions to the protein solution induces morphological changes in the micellar structure by transforming the 3D micelles into objects of reduced dimensionality of 2, with fractal-like characteristics (including Gaussian-chain-like) or, alternatively, platelet-like structures.

View Accepted Manuscript (DOE)

Research Organization:: Ames Laboratory (AMES), Ames, IA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: AC02-07CH11358

OSTI ID:: 1227436

Alternate ID(s):: OSTI ID: 1172423

Report Number(s):: IS-J--8577

Journal Information:: Langmuir, Journal Name: Langmuir Journal Issue: 9 Vol. 31; ISSN 0743-7463

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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