Strains for the production of flavonoids from glucose

Stephanopoulos, Gregory; Santos, Christine; Koffas, Mattheos

Strains for the production of flavonoids from glucose

Patent · Fri Nov 13 04:00:00 EST 2015

OSTI ID:1225786

Stephanopoulos, Gregory; Santos, Christine; Koffas, Mattheos

The invention relates to the production of flavonoids and flavonoid precursors in cells through recombinant expression of tyrosine ammonia lyase (TAL), 4-coumarate:CoA ligase (4CL), chalcone synthase (CHS), and chalcone isomerase (CHI).

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Research Organization:: Massachusetts Institute of Technology, Cambridge, MA, (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FC36-07GO17058

Assignee:: Massachusetts Institute of Technology

Patent Number(s):: 9,181,539

Application Number:: 13/101,462

OSTI ID:: 1225786

Country of Publication:: United States

Language:: English

References (38)

Improved production of p-hydroxycinnamic acid from tyrosine using a novel thermostable phenylalanine/tyrosine ammonia lyase enzyme Xue, Zhixiong; McCluskey, Michael; Cantera, Keith Enzyme and Microbial Technology, Vol. 42, Issue 1, p. 58-64 https://doi.org/10.1016/j.enzmictec.2007.07.025	journal	December 2007
Exploring Recombinant Flavonoid Biosynthesis in Metabolically Engineered Escherichia coli Watts, Kevin T.; Lee, Pyung Cheon; Schmidt-Dannert, Claudia ChemBioChem, Vol. 5, Issue 4, p. 500-507 https://doi.org/10.1002/cbic.200300783	journal	April 2004
Identification, characterization and functional expression of a tyrosine ammonia-lyase and its mutants from the photosynthetic bacterium Rhodobacter sphaeroides Xue, Zhixiong; McCluskey, Michael; Cantera, Keith Journal of Industrial Microbiology & Biotechnology, Vol. 34, Issue 9 https://doi.org/10.1007/s10295-007-0229-1	journal	June 2007
GeMS: an advanced software package for designing synthetic genes Jayaraj, S. Nucleic Acids Research, Vol. 33, Issue 9 https://doi.org/10.1093/nar/gki614	journal	May 2005
Conversion of a β-Ketoacyl Synthase to a Malonyl Decarboxylase by Replacement of the Active-Site Cysteine with Glutamine Witkowski, Andrzej; Joshi, Anil K.; Lindqvist, Ylva Biochemistry, Vol. 38, Issue 36, p. 11643-11650 https://doi.org/10.1021/bi990993h	journal	September 1999
Metabolic engineering and applications of flavonoids Forkmann, Gert; Martens, Stefan Current Opinion in Biotechnology, Vol. 12, Issue 2, p. 155-160 https://doi.org/10.1016/S0958-1669(00)00192-0	journal	April 2001
Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids Broun, Pierre; Shanklin, John; Whittle, Ed Science, Vol. 282, Issue 5392, p. 1315-1317 https://doi.org/10.1126/science.282.5392.1315	journal	November 1998
Development of a Combined Biological and Chemical Process for Production of Industrial Aromatics from Renewable Resources Sariaslani, F. Sima Annual Review of Microbiology, Vol. 61, Issue 1 https://doi.org/10.1146/annurev.micro.61.080706.093248	journal	October 2007
Optimization of a heterologous pathway for the production of flavonoids from glucose Santos, Christine Nicole S.; Koffas, Mattheos; Stephanopoulos, Gregory Metabolic Engineering, Vol. 13, Issue 4, p. 392-400 https://doi.org/10.1016/j.ymben.2011.02.002	journal	July 2011
Prediction of protein function from protein sequence and structure Whisstock, James C.; Lesk, Arthur M. Quarterly Reviews of Biophysics, Vol. 36, Issue 3, p. 307-340 https://doi.org/10.1017/S0033583503003901	journal	January 1999
Efficient production of (2S)-flavanones by Escherichia coli containing an artificial biosynthetic gene cluster Miyahisa, Ikuo; Kaneko, Masafumi; Funa, Nobutaka Applied Microbiology and Biotechnology, Vol. 68, Issue 4 https://doi.org/10.1007/s00253-005-1916-3	journal	March 2005
Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different Seffernick, J. L.; de Souza, M. L.; Sadowsky, M. J. Journal of Bacteriology, Vol. 183, Issue 8, p. 2405-2410 https://doi.org/10.1128/JB.183.8.2405-2410.2001	journal	April 2001
Heterologous production of flavanones in Escherichia coli : potential for combinatorial biosynthesis of flavonoids in bacteria Kaneko, Masafumi; Hwang, Eui Il; Ohnishi, Yasuo Journal of Industrial Microbiology and Biotechnology, Vol. 30, Issue 8 https://doi.org/10.1007/s10295-003-0061-1	journal	August 2003
Discovery of a Substrate Selectivity Switch in Tyrosine Ammonia-Lyase, a Member of the Aromatic Amino Acid Lyase Family Watts, Kevin T.; Mijts, Benjamin N.; Lee, Pyung Cheon Chemistry & Biology, Vol. 13, Issue 12, p. 1317-1326 https://doi.org/10.1016/j.chembiol.2006.10.008	journal	December 2006
Production of p-hydroxycinnamic acid from glucose in Saccharomyces cerevisiae and Escherichia coli by expression of heterologous genes from plants and fungi Vannelli, Todd; Wei Qi, Wei; Sweigard, James Metabolic Engineering, Vol. 9, Issue 2, p. 142-151 https://doi.org/10.1016/j.ymben.2006.11.001	journal	March 2007
Strain Improvement of Recombinant Escherichia coli for Efficient Production of Plant Flavonoids Leonard, Effendi; Yan, Yajun; Fowler, Zachary L. Molecular Pharmaceutics, Vol. 5, Issue 2 https://doi.org/10.1021/mp7001472	journal	January 2008
Contributions of conserved serine and tyrosine residues to catalysis, ligand binding, and cofactor processing in the active site of tyrosine ammonia lyase Schroeder, Amy C.; Kumaran, Sangaralingam; Hicks, Leslie M. Phytochemistry, Vol. 69, Issue 7, p. 1496-1506 https://doi.org/10.1016/j.phytochem.2008.02.007	journal	May 2008
Developments in Directed Evolution for Improving Enzyme Functions Sen, S.; Venkata Dasu, V.; Mandal, B. Applied Biochemistry and Biotechnology, Vol. 143, Issue 3, p. 212-223 https://doi.org/10.1007/s12010-007-8003-4	journal	August 2007
Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design Chica, Roberto A.; Doucet, Nicolas; Pelletier, Joelle N. Current Opinion in Biotechnology, Vol. 16, Issue 4, p. 378-384 https://doi.org/10.1016/j.copbio.2005.06.004	journal	August 2005
Changes in the Intracellular Concentration of Acetyl-CoA and Malonyl-CoA in Relation to the Carbon and Energy Metabolism of Escherichia coli K12 Takamura, Y.; Nomura, G. Microbiology, Vol. 134, Issue 8 https://doi.org/10.1099/00221287-134-8-2249	journal	August 1988
Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli Kane, James F. Current Opinion in Biotechnology, Vol. 6, Issue 5, p. 494-500 https://doi.org/10.1016/0958-1669(95)80082-4	journal	January 1995
A "Silent" Polymorphism in the MDR1 Gene Changes Substrate Specificity Kimchi-Sarfaty, C.; Oh, J. M.; Kim, I.-W. Science, Vol. 315, Issue 5811, p. 525-528 https://doi.org/10.1126/science.1135308	journal	January 2007
Human Catechol-O-Methyltransferase Haplotypes Modulate Protein Expression by Altering mRNA Secondary Structure Nackley, A. G.; Shabalina, S. A.; Tchivileva, I. E. Science, Vol. 314, Issue 5807, p. 1930-1933 https://doi.org/10.1126/science.1131262	journal	December 2006
Polypeptide Release Factors in Prokaryotes and Eukaryotes Kisselev, Lev Structure, Vol. 10, Issue 1, p. 8-9 https://doi.org/10.1016/S0969-2126(01)00703-1	journal	January 2002
Increased Malonyl Coenzyme A Biosynthesis by Tuning the Escherichia coli Metabolic Network and Its Application to Flavanone Production Fowler, Z. L.; Gikandi, W. W.; Koffas, M. A. G. Applied and Environmental Microbiology, Vol. 75, Issue 18 https://doi.org/10.1128/AEM.00270-09	journal	July 2009
Total synthesis of long DNA sequences: Synthesis of a contiguous 32-kb polyketide synthase gene cluster Kodumal, S. J.; Patel, K. G.; Reid, R. Proceedings of the National Academy of Sciences, Vol. 101, Issue 44, p. 15573-15578 https://doi.org/10.1073/pnas.0406911101	journal	October 2004
Bioavailability and Health Effects of Dietary Flavonols in Man Hollman, Peter C. H.; Katan, Martijn B. Archives of Toxicology https://doi.org/10.1007/978-3-642-46856-8_21	book	January 1998
Engineering Central Metabolic Pathways for High-Level Flavonoid Production in Escherichia coli Leonard, E.; Lim, K. -H.; Saw, P. -N. Applied and Environmental Microbiology, Vol. 73, Issue 12 https://doi.org/10.1128/AEM.00200-07	journal	April 2007
Engineering and characterization of a superfolder green fluorescent protein Pédelacq, Jean-Denis; Cabantous, Stéphanie; Tran, Timothy Nature Biotechnology, Vol. 24, Issue 1, p. 79-88 https://doi.org/10.1038/nbt1172	journal	December 2005
Tuning genetic control through promoter engineering Alper, H.; Fischer, C.; Nevoigt, E. Proceedings of the National Academy of Sciences, Vol. 102, Issue 36, p. 12678-12683 https://doi.org/10.1073/pnas.0504604102	journal	August 2005
Silent Polymorphisms Speak: How They Affect Pharmacogenomics and the Treatment of Cancer Sauna, Z. E.; Kimchi-Sarfaty, C.; Ambudkar, S. V. Cancer Research, Vol. 67, Issue 20, p. 9609-9612 https://doi.org/10.1158/0008-5472.CAN-07-2377	journal	October 2007
Mutagenesis of the Bacterial RNA Polymerase Alpha Subunit for Improvement of Complex Phenotypes Klein-Marcuschamer, D.; Santos, C. N. S.; Yu, H. Applied and Environmental Microbiology, Vol. 75, Issue 9 https://doi.org/10.1128/AEM.01888-08	journal	February 2009
Advances in flavonoid research since 1992 Harborne, Jeffrey B.; Williams, Christine A. Phytochemistry, Vol. 55, Issue 6, p. 481-504 https://doi.org/10.1016/S0031-9422(00)00235-1	journal	November 2000
Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes Pfleger, Brian F.; Pitera, Douglas J.; Smolke, Christina D. Nature Biotechnology, Vol. 24, Issue 8 https://doi.org/10.1038/nbt1226	journal	July 2006
A Single Mutation Converts a Novel Phosphotyrosine Binding Domain into a Dual-specificity Phosphatase Wishart, Matthew J.; Denu, John M.; Williams, John A. Journal of Biological Chemistry, Vol. 270, Issue 45, p. 26782-26785 https://doi.org/10.1074/jbc.270.45.26782	journal	November 1995
Biosynthesis and biotechnological production of flavanones: current state and perspectives Fowler, Zachary L.; Koffas, Mattheos A. G. Applied Microbiology and Biotechnology, Vol. 83, Issue 5 https://doi.org/10.1007/s00253-009-2039-z	journal	May 2009
Flavonoid intake and coronary mortality in Finland: a cohort study Knekt, P.; Jarvinen, R.; Reunanen, A. BMJ, Vol. 312, Issue 7029 https://doi.org/10.1136/bmj.312.7029.478	journal	February 1996
Practical limits of function prediction Devos, Damien; Valencia, Alfonso Proteins: Structure, Function, and Genetics, Vol. 41, Issue 1, p. 98-107 https://doi.org/10.1002/1097-0134(20001001)41:1<98::AID-PROT120>3.0.CO;2-S	journal	January 2000

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