Strains for the production of flavonoids from glucose

Title: Strains for the production of flavonoids from glucose

Abstract

The invention relates to the production of flavonoids and flavonoid precursors in cells through recombinant expression of tyrosine ammonia lyase (TAL), 4-coumarate:CoA ligase (4CL), chalcone synthase (CHS), and chalcone isomerase (CHI).

Inventors:: Stephanopoulos, Gregory; Santos, Christine; Koffas, Mattheos

Publication Date:: 2015-11-13

Research Org.:: Massachusetts Institute of Technology, Cambridge, MA, (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1225786

Patent Number(s):: 9,181,539

Application Number:: 13/101,462

Assignee:: Massachusetts Institute of Technology

DOE Contract Number:: FC36-07GO17058

Resource Type:: Patent

Resource Relation:: Patent File Date: 2011 May 05

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Stephanopoulos, Gregory, Santos, Christine, and Koffas, Mattheos. Strains for the production of flavonoids from glucose.  United States: N. p., 2015. 
        Web.

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                    Stephanopoulos, Gregory, Santos, Christine, & Koffas, Mattheos. Strains for the production of flavonoids from glucose.  United States.

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                    Stephanopoulos, Gregory, Santos, Christine, and Koffas, Mattheos. Fri .  
        "Strains for the production of flavonoids from glucose".  United States.  https://www.osti.gov/servlets/purl/1225786.

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@article{osti_1225786,

  title        = {Strains for the production of flavonoids from glucose},

  author       = {Stephanopoulos, Gregory and Santos, Christine and Koffas, Mattheos},

  abstractNote = {The invention relates to the production of flavonoids and flavonoid precursors in cells through recombinant expression of tyrosine ammonia lyase (TAL), 4-coumarate:CoA ligase (4CL), chalcone synthase (CHS), and chalcone isomerase (CHI).},

  doi          = {},

  url          = {https://www.osti.gov/biblio/1225786},
  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2015},

  month        = {11}

}

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Similar Records

Title: Strains for the production of flavonoids from glucose

Abstract

Citation Formats

Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids journal, November 1998

Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design journal, August 2005

Practical limits of function prediction journal, January 2000

A "Silent" Polymorphism in the MDR1 Gene Changes Substrate Specificity journal, January 2007

Polypeptide Release Factors in Prokaryotes and Eukaryotes journal, January 2002

Human Catechol-O-Methyltransferase Haplotypes Modulate Protein Expression by Altering mRNA Secondary Structure journal, December 2006

Silent Polymorphisms Speak: How They Affect Pharmacogenomics and the Treatment of Cancer journal, October 2007

Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different journal, April 2001

Developments in Directed Evolution for Improving Enzyme Functions journal, August 2007

Prediction of protein function from protein sequence and structure journal, January 1999

A Single Mutation Converts a Novel Phosphotyrosine Binding Domain into a Dual-specificity Phosphatase journal, November 1995

Conversion of a β-Ketoacyl Synthase to a Malonyl Decarboxylase by Replacement of the Active-Site Cysteine with Glutamine journal, September 1999

Tuning genetic control through promoter engineering journal, August 2005

Metabolic engineering and applications of flavonoids journal, April 2001

Biosynthesis and biotechnological production of flavanones: current state and perspectives journal, May 2009

Increased Malonyl Coenzyme A Biosynthesis by Tuning the Escherichia coli Metabolic Network and Its Application to Flavanone Production journal, July 2009

Advances in flavonoid research since 1992 journal, November 2000

GeMS: an advanced software package for designing synthetic genes journal, May 2005

Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli journal, January 1995

Heterologous production of flavanones in Escherichia coli : potential for combinatorial biosynthesis of flavonoids in bacteria journal, August 2003

Mutagenesis of the Bacterial RNA Polymerase Alpha Subunit for Improvement of Complex Phenotypes journal, February 2009

Flavonoid intake and coronary mortality in Finland: a cohort study journal, February 1996

Total synthesis of long DNA sequences: Synthesis of a contiguous 32-kb polyketide synthase gene cluster journal, October 2004

Engineering Central Metabolic Pathways for High-Level Flavonoid Production in Escherichia coli journal, April 2007

Strain Improvement of Recombinant Escherichia coli for Efficient Production of Plant Flavonoids journal, January 2008

Efficient production of (2S)-flavanones by Escherichia coli containing an artificial biosynthetic gene cluster journal, March 2005

Engineering and characterization of a superfolder green fluorescent protein journal, December 2005

Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes journal, July 2006

Optimization of a heterologous pathway for the production of flavonoids from glucose journal, July 2011

Development of a Combined Biological and Chemical Process for Production of Industrial Aromatics from Renewable Resources journal, October 2007

Contributions of conserved serine and tyrosine residues to catalysis, ligand binding, and cofactor processing in the active site of tyrosine ammonia lyase journal, May 2008

Changes in the Intracellular Concentration of Acetyl-CoA and Malonyl-CoA in Relation to the Carbon and Energy Metabolism of Escherichia coli K12 journal, August 1988

Production of p-hydroxycinnamic acid from glucose in Saccharomyces cerevisiae and Escherichia coli by expression of heterologous genes from plants and fungi journal, March 2007

Discovery of a Substrate Selectivity Switch in Tyrosine Ammonia-Lyase, a Member of the Aromatic Amino Acid Lyase Family journal, December 2006

Exploring Recombinant Flavonoid Biosynthesis in Metabolically Engineered Escherichia coli journal, April 2004

Identification, characterization and functional expression of a tyrosine ammonia-lyase and its mutants from the photosynthetic bacterium Rhodobacter sphaeroides journal, June 2007

Improved production of p-hydroxycinnamic acid from tyrosine using a novel thermostable phenylalanine/tyrosine ammonia lyase enzyme journal, December 2007

Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids
journal, November 1998

Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design
journal, August 2005

Practical limits of function prediction
journal, January 2000

A "Silent" Polymorphism in the MDR1 Gene Changes Substrate Specificity
journal, January 2007

Polypeptide Release Factors in Prokaryotes and Eukaryotes
journal, January 2002

Human Catechol-O-Methyltransferase Haplotypes Modulate Protein Expression by Altering mRNA Secondary Structure
journal, December 2006

Silent Polymorphisms Speak: How They Affect Pharmacogenomics and the Treatment of Cancer
journal, October 2007

Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different
journal, April 2001

Developments in Directed Evolution for Improving Enzyme Functions
journal, August 2007

Prediction of protein function from protein sequence and structure
journal, January 1999

A Single Mutation Converts a Novel Phosphotyrosine Binding Domain into a Dual-specificity Phosphatase
journal, November 1995

Conversion of a β-Ketoacyl Synthase to a Malonyl Decarboxylase by Replacement of the Active-Site Cysteine with Glutamine
journal, September 1999

Tuning genetic control through promoter engineering
journal, August 2005

Metabolic engineering and applications of flavonoids
journal, April 2001

Biosynthesis and biotechnological production of flavanones: current state and perspectives
journal, May 2009

Increased Malonyl Coenzyme A Biosynthesis by Tuning the Escherichia coli Metabolic Network and Its Application to Flavanone Production
journal, July 2009

Advances in flavonoid research since 1992
journal, November 2000

GeMS: an advanced software package for designing synthetic genes
journal, May 2005

Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli
journal, January 1995

Heterologous production of flavanones in Escherichia coli : potential for combinatorial biosynthesis of flavonoids in bacteria
journal, August 2003

Mutagenesis of the Bacterial RNA Polymerase Alpha Subunit for Improvement of Complex Phenotypes
journal, February 2009

Flavonoid intake and coronary mortality in Finland: a cohort study
journal, February 1996

Total synthesis of long DNA sequences: Synthesis of a contiguous 32-kb polyketide synthase gene cluster
journal, October 2004

Engineering Central Metabolic Pathways for High-Level Flavonoid Production in Escherichia coli
journal, April 2007

Strain Improvement of Recombinant Escherichia coli for Efficient Production of Plant Flavonoids
journal, January 2008

Efficient production of (2S)-flavanones by Escherichia coli containing an artificial biosynthetic gene cluster
journal, March 2005

Engineering and characterization of a superfolder green fluorescent protein
journal, December 2005

Combinatorial engineering of intergenic regions in operons tunes expression of multiple genes
journal, July 2006

Optimization of a heterologous pathway for the production of flavonoids from glucose
journal, July 2011

Development of a Combined Biological and Chemical Process for Production of Industrial Aromatics from Renewable Resources
journal, October 2007

Contributions of conserved serine and tyrosine residues to catalysis, ligand binding, and cofactor processing in the active site of tyrosine ammonia lyase
journal, May 2008

Changes in the Intracellular Concentration of Acetyl-CoA and Malonyl-CoA in Relation to the Carbon and Energy Metabolism of Escherichia coli K12
journal, August 1988

Production of p-hydroxycinnamic acid from glucose in Saccharomyces cerevisiae and Escherichia coli by expression of heterologous genes from plants and fungi
journal, March 2007

Discovery of a Substrate Selectivity Switch in Tyrosine Ammonia-Lyase, a Member of the Aromatic Amino Acid Lyase Family
journal, December 2006

Exploring Recombinant Flavonoid Biosynthesis in Metabolically Engineered Escherichia coli
journal, April 2004

Identification, characterization and functional expression of a tyrosine ammonia-lyase and its mutants from the photosynthetic bacterium Rhodobacter sphaeroides
journal, June 2007

Improved production of p-hydroxycinnamic acid from tyrosine using a novel thermostable phenylalanine/tyrosine ammonia lyase enzyme
journal, December 2007