A high-transparency, micro-patternable chip for X-ray diffraction analysis of microcrystals under native growth conditions

Murray, Thomas D.; Lyubimov, Artem Y.; Ogata, Craig M.; Vo, Huy; Uervirojnangkoorn, Monarin; Brunger, Axel T.; Berger, James M.

doi:10.1107/S1399004715015011

A high-transparency, micro-patternable chip for X-ray diffraction analysis of microcrystals under native growth conditions

Journal Article · Tue Aug 11 00:00:00 EDT 2015 · Acta Crystallographica. Section D: Biological Crystallography (Online)

DOI:https://doi.org/10.1107/S1399004715015011· OSTI ID:1224900

Murray, Thomas D. ^[1]; Lyubimov, Artem Y. ^[2]; Ogata, Craig M. ^[3]; Vo, Huy ^[4]; Uervirojnangkoorn, Monarin ^[2]; Brunger, Axel T. ^[2]; Berger, James M. ^[5]

Univ. of California, Berkeley, CA (United States); John Hopkins Univ. School of Medicine, Baltimore, MD (United States)
Stanford Univ., Stanford, CA (United States)
Argonne National Lab. (ANL), Argonne, IL (United States)
John Hopkins Univ., Baltimore, MD (United States)
John Hopkins Univ. School of Medicine, Baltimore, MD (United States)

Microcrystals present a significant impediment to the determination of macromolecular structures by X-ray diffraction methods. Although microfocus synchrotron beamlines and X-ray free-electron lasers (XFELs) can enable the collection of interpretable diffraction data from microcrystals, there is a need for efficient methods of harvesting small volumes (<2 µl) of microcrystals grown under common laboratory formats and delivering them to an X-ray beam source under native growth conditions. One approach that shows promise in overcoming the challenges intrinsic to microcrystal analysis is to pair so-called `fixed-target' sample-delivery devices with microbeam-based X-ray diffraction methods. However, to record weak diffraction patterns it is necessary to fabricate devices from X-ray-transparent materials that minimize background scattering. Presented here is the design of a new micro-diffraction device consisting of three layers fabricated from silicon nitride, photoresist and polyimide film. The chip features low X-ray scattering and X-ray absorption properties, and uses a customizable blend of hydrophobic and hydrophilic surface patterns to help localize microcrystals to defined regions. Microcrystals in their native growth conditions can be loaded into the chips with a standard pipette, allowing data collection at room temperature. Diffraction data collected from hen egg-white lysozyme microcrystals (10–15 µm) loaded into the chips yielded a complete, high-resolution (<1.6 Å) data set sufficient to determine a high-quality structure by molecular replacement. In addition, the features of the chip allow the rapid and user-friendly analysis of microcrystals grown under virtually any laboratory format at microfocus synchrotron beamlines and XFELs.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States); SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: AC02-06CH11357; AC03-76SF00515

OSTI ID:: 1224900

Alternate ID(s):: OSTI ID: 1224914
OSTI ID: 22420137
OSTI ID: 1237945
OSTI ID: 1257456

Journal Information:: Acta Crystallographica. Section D: Biological Crystallography (Online), Journal Name: Acta Crystallographica. Section D: Biological Crystallography (Online) Journal Issue: 10 Vol. 71; ISSN 1399-0047; ISSN ABCRE6

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

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High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source Martin-Garcia, Jose M.; Zhu, Lan; Mendez, Derek International Union of Crystallography (IUCr) https://doi.org/10.1107/s205225251900263x/ti5014sup1.pdf	text	January 2019
On-chip crystallization for serial crystallography experiments and on-chip ligand-binding studies Lieske, Julia; Cerv, Maximilian; Kreida, Stefan IUCrJ, Vol. 6, Issue 4 https://doi.org/10.1107/s2052252519007395	journal	June 2019
On-chip crystallization for serial crystallography experiments and on-chip ligand-binding studies Lieske, Julia; Cerv, Maximilian; Kreida, Stefan International Union of Crystallography (IUCr) https://doi.org/10.1107/s2052252519007395/mf5034sup1.pdf	text	January 2019
Applications of thin-film sandwich crystallization platforms Axford, Danny; Aller, Pierre; Sanchez-Weatherby, Juan Acta Crystallographica Section F Structural Biology Communications, Vol. 72, Issue 4 https://doi.org/10.1107/s2053230x16004386	journal	March 2016
Fixed target combined with spectral mapping: approaching 100% hit rates for serial crystallography Oghbaey, Saeed; Sarracini, Antoine; Ginn, Helen M. Acta Crystallographica Section D Structural Biology, Vol. 72, Issue 8, p. 944-955 https://doi.org/10.1107/s2059798316010834	journal	July 2016
Low-dose fixed-target serial synchrotron crystallography Owen, Robin L.; Axford, Danny; Sherrell, Darren A. Acta Crystallographica Section D Structural Biology, Vol. 73, Issue 4, p. 373-378 https://doi.org/10.1107/s2059798317002996	journal	March 2017
Crystallography on a chip – without the chip: sheet-on-sheet sandwich Doak, R. Bruce; Nass Kovacs, Gabriela; Gorel, Alexander Acta Crystallographica Section D Structural Biology, Vol. 74, Issue 10 https://doi.org/10.1107/s2059798318011634	journal	October 2018
Sample delivery for serial crystallography at free-electron lasers and synchrotrons Grünbein, Marie Luise; Nass Kovacs, Gabriela Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 2 https://doi.org/10.1107/s205979831801567x	journal	January 2019
ZOO : an automatic data-collection system for high-throughput structure analysis in protein microcrystallography Hirata, Kunio; Yamashita, Keitaro; Ueno, Go Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 2 https://doi.org/10.1107/s2059798318017795	journal	January 2019
Strategies for sample delivery for femtosecond crystallography Martiel, Isabelle; Müller-Werkmeister, Henrike M.; Cohen, Aina E. Acta Crystallographica Section D Structural Biology, Vol. 75, Issue 2 https://doi.org/10.1107/s2059798318017953	journal	February 2019
Strategies for sample delivery for femtosecond crystallography Martiel, Isabelle; Müller-Werkmeister, Henrike M.; Cohen, Aina E. International Union of Crystallography (IUCr) https://doi.org/10.1107/s2059798318017953/ba5296sup1.pdf	text	January 2019
In situ crystallography as an emerging method for structure solution of membrane proteins: the case of CCR2A Cheng, Robert; Huang, Chia‐Ying; Hennig, Michael The FEBS Journal, Vol. 287, Issue 5 https://doi.org/10.1111/febs.15098	journal	November 2019
A guide to sample delivery systems for serial crystallography Zhao, Feng‐Zhu; Zhang, Bin; Yan, Er‐Kai The FEBS Journal, Vol. 286, Issue 22 https://doi.org/10.1111/febs.15099	journal	August 2019
Room-Temperature Macromolecular Crystallography Using a Micro-Patterned Silicon Chip with Minimal Background Scattering Roedig, Philip; Duman, Ramona; Sanchez-Weatherby, Juan Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2016-02690	text	January 2016
On-chip crystallization for serial crystallography experiments and on-chip ligand-binding studies Lieske, Julia; Cerv, Maximilian; Kreida, Stefan Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2019-02620	text	January 2019
A fixed-target platform for serial femtosecond crystallography in a hydrated environment Shelby, M. L.; Gilbile, D.; Grant, T. D. Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2019-05316	text	January 2020
Advances in X-ray free electron laser (XFEL) diffraction data processing applied to the crystal structure of the synaptotagmin-1 / SNARE complex Lyubimov, Artem Y.; Uervirojnangkoorn, Monarin; Zeldin, Oliver B. eLife, Vol. 5 https://doi.org/10.7554/elife.18740	journal	October 2016

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A high-transparency, micro-patternable chip for X-ray diffraction analysis of microcrystals under native growth conditions

Journal Article · Sat Sep 26 00:00:00 EDT 2015 · Acta Crystallographica. Section D: Biological Crystallography · OSTI ID:22420137

A high-transparency, micro-patternable chip for X-ray diffraction analysis of microcrystals under native growth conditions

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A high-transparency, micro-patternable chip for X-ray diffraction analysis of microcrystals under native growth conditions

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