Structures of Escherichia coli DNA adenine methyltransferase (Dam) in complex with a non-GATC sequence: Potential implications for methylation-independent transcriptional repression

Horton, John R.; Zhang, Xing; Blumenthal, Robert M.; Cheng, Xiaodong

doi:10.1093/nar/gkv251

Structures of Escherichia coli DNA adenine methyltransferase (Dam) in complex with a non-GATC sequence: Potential implications for methylation-independent transcriptional repression

Journal Article · Mon Apr 06 04:00:00 EDT 2015 · Nucleic Acids Research

DOI:https://doi.org/10.1093/nar/gkv251· OSTI ID:1223724

Horton, John R. ^[1]; Zhang, Xing ^[1]; Blumenthal, Robert M. ^[2]; Cheng, Xiaodong ^[1]

Emory Univ. School of Medicine, Atlanta, GA (United States)
The Univ. of Toledo College of Medicine and Life Sciences, Toledo, OH (United States)

DNA adenine methyltransferase (Dam) is widespread and conserved among the γ-proteobacteria. Methylation of the Ade in GATC sequences regulates diverse bacterial cell functions, including gene expression, mismatch repair and chromosome replication. Dam also controls virulence in many pathogenic Gram-negative bacteria. An unexplained and perplexing observation about Escherichia coli Dam (EcoDam) is that there is no obvious relationship between the genes that are transcriptionally responsive to Dam and the promoter-proximal presence of GATC sequences. Here, we demonstrate that EcoDam interacts with a 5-base pair non-cognate sequence distinct from GATC. The crystal structure of a non-cognate complex allowed us to identify a DNA binding element, GTYTA/TARAC (where Y = C/T and R = A/G). This element immediately flanks GATC sites in some Dam-regulated promoters, including the Pap operon which specifies pyelonephritis-associated pili. In addition, Dam interacts with near-cognate GATC sequences (i.e. 3/4-site ATC and GAT). All together, these results imply that Dam, in addition to being responsible for GATC methylation, could also function as a methylation-independent transcriptional repressor.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: AC02-06CH11357; W-31109-ENG-38

OSTI ID:: 1223724

Alternate ID(s):: OSTI ID: 1247332

Journal Information:: Nucleic Acids Research, Journal Name: Nucleic Acids Research Journal Issue: 8 Vol. 43; ISSN 0305-1048

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

References (72)

Transition from EcoDam to T4Dam DNA Recognition Mechanism without Loss of Activity and Specificity Elsawy, Hany; Podobinschi, Sveatoslav; Chahar, Sanjay ChemBioChem, Vol. 10, Issue 15 https://doi.org/10.1002/cbic.200900441	journal	October 2009
Complementary specificity of restriction endonucleases of Diplococcus pneumoniae with respect to DNA methylation Lacks, Sanford; Greenberg, Bill Journal of Molecular Biology, Vol. 114, Issue 1 https://doi.org/10.1016/0022-2836(77)90289-3	journal	July 1977
Sequence specificity of the P1 modification methylase (M·Eco P1) and the DNA methylase (M·Eco dam) controlled by the Escherichia coli dam gene Hattman, Stanley; Brooks, Joan E.; Masurekar, Malthi Journal of Molecular Biology, Vol. 126, Issue 3 https://doi.org/10.1016/0022-2836(78)90046-3	journal	December 1978
The rate of Dam-mediated DNA adenine methylation in Escherichia coli Campbell, Joseph L.; Kleckner, Nancy Gene, Vol. 74, Issue 1 https://doi.org/10.1016/0378-1119(88)90283-1	journal	December 1988
Nature/Nurture and Creativity Vartanian, O. Encyclopedia of Creativity https://doi.org/10.1016/b978-0-12-375038-9.00158-8	book	January 2011
Transition from Nonspecific to Specific DNA Interactions along the Substrate-Recognition Pathway of Dam Methyltransferase Horton, John R.; Liebert, Kirsten; Hattman, Stanley Cell, Vol. 121, Issue 3 https://doi.org/10.1016/j.cell.2005.02.021	journal	May 2005
Structure and Substrate Recognition of the Escherichia coli DNA Adenine Methyltransferase Horton, John R.; Liebert, Kirsten; Bekes, Miklos Journal of Molecular Biology, Vol. 358, Issue 2 https://doi.org/10.1016/j.jmb.2006.02.028	journal	April 2006
Competitive Lrp and Dam Assembly at the pap Regulatory Region: Implications for Mechanisms of Epigenetic Regulation Peterson, Stacey N.; Reich, Norbert O. Journal of Molecular Biology, Vol. 383, Issue 1 https://doi.org/10.1016/j.jmb.2008.07.086	journal	October 2008
DNA Looping Provides for “Intersegmental Hopping” by Proteins: A Mechanism for Long-Range Site Localization Pollak, Adam J.; Chin, Aaron T.; Brown, Frank L. H. Journal of Molecular Biology, Vol. 426, Issue 21 https://doi.org/10.1016/j.jmb.2014.08.002	journal	October 2014
Dam methylation: coordinating cellular processes Løbner-Olesen, Anders; Skovgaard, Ole; Marinus, Martin G. Current Opinion in Microbiology, Vol. 8, Issue 2 https://doi.org/10.1016/j.mib.2005.02.009	journal	April 2005
Dam methylation regulates the expression of SPI-5-encoded sopB gene in Salmonella enterica serovar Typhimurium Giacomodonato, Mónica N.; Llana, Mariángeles Noto; Castañeda, María del Rosario Aya Microbes and Infection, Vol. 16, Issue 8 https://doi.org/10.1016/j.micinf.2014.03.009	journal	August 2014
Protein production by auto-induction in high-density shaking cultures Studier, F. William Protein Expression and Purification, Vol. 41, Issue 1, p. 207-234 https://doi.org/10.1016/j.pep.2005.01.016	journal	May 2005
Timing and targeting: The biological functions of Dam methylation in E. coli Messer, Walter; Noyer-Weidner, Mario Cell, Vol. 54, Issue 6 https://doi.org/10.1016/s0092-8674(88)90911-7	journal	September 1988
Effects of global regulatory proteins and environmental conditionson fimbrial gene expression of F1651 and F1652 producedby Escherichia coli causing septicaemia in pigs Daigle, France; Forget, Céline; Martin, Christine Research in Microbiology, Vol. 151, Issue 7 https://doi.org/10.1016/s0923-2508(00)00226-6	journal	September 2000
Structure and Specificity of the Vertebrate Anti-Mutator Uracil-DNA Glycosylase SMUG1 Wibley, Jane E. A.; Waters, Timothy R.; Haushalter, Karl Molecular Cell, Vol. 11, Issue 6 https://doi.org/10.1016/s1097-2765(03)00235-1	journal	June 2003
The Mechanism by which DNA Adenine Methylase and PapI Activate the Pap Epigenetic Switch Hernday, Aaron D.; Braaten, Bruce A.; Low, David A. Molecular Cell, Vol. 12, Issue 4 https://doi.org/10.1016/s1097-2765(03)00383-6	journal	October 2003
Structure of a DNA base-excision product resembling a cisplatin inter-strand adduct Barrett, Tracey E.; Savva, Renos; Barlow, Tom Nature Structural & Molecular Biology, Vol. 5, Issue 8 https://doi.org/10.1038/1394	journal	August 1998
Structure of the bacteriophage T4 DNA adenine methyltransferase Yang, Zhe; Horton, John R.; Zhou, Lan Nature Structural & Molecular Biology, Vol. 10, Issue 10 https://doi.org/10.1038/nsb973	journal	August 2003
Role of SeqA and Dam in Escherichia coli gene expression: A global/microarray analysis Lobner-Olesen, A.; Marinus, M. G.; Hansen, F. G. Proceedings of the National Academy of Sciences, Vol. 100, Issue 8 https://doi.org/10.1073/pnas.0538053100	journal	April 2003
Increased subtlety of transcription factor binding increases complexity of genome regulation von Hippel, Peter H. Proceedings of the National Academy of Sciences, Vol. 111, Issue 49 https://doi.org/10.1073/pnas.1418978111	journal	December 2014
Functional Characterization of Escherichia coli DNA Adenine Methyltransferase, a Novel Target for Antibiotics Mashhoon, Neda; Carroll, Michael; Pruss, Cynthia Journal of Biological Chemistry, Vol. 279, Issue 50 https://doi.org/10.1074/jbc.m408182200	journal	September 2004
The M.EcoRV DNA-(Adenine N ⁶ )-methyltransferase Uses DNA Bending for Recognition of an Expanded EcoDam Recognition Site Jurkowski, Tomasz P.; Anspach, Nils; Kulishova, Liliya Journal of Biological Chemistry, Vol. 282, Issue 51 https://doi.org/10.1074/jbc.m706933200	journal	October 2007
Modulation of Escherichia coli DNA Methyltransferase Activity by Biologically Derived GATC-flanking Sequences Coffin, Stephanie R.; Reich, Norbert O. Journal of Biological Chemistry, Vol. 283, Issue 29 https://doi.org/10.1074/jbc.m802502200	journal	May 2008
The Isolation and Characterization of the Esherichia Coli DNA adenine methylase (dam) gene Brooks, Joan E.; Blumenthal, Robert M.; Gingeras, Thomas R. Nucleic Acids Research, Vol. 11, Issue 3 https://doi.org/10.1093/nar/11.3.837	journal	January 1983
M Hha l binds tightly to substrates containing mismatches at the target base Klimašauskas, Saulius; Roberts, Richard J. Nucleic Acids Research, Vol. 23, Issue 8 https://doi.org/10.1093/nar/23.8.1388	journal	January 1995
Features and development of Coot Emsley, P.; Lohkamp, B.; Scott, W. G. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4 https://doi.org/10.1107/s0907444910007493	journal	March 2010
Crystallography & NMR System: A New Software Suite for Macromolecular Structure Determination Brünger, A. T.; Adams, P. D.; Clore, G. M. Acta Crystallographica Section D Biological Crystallography, Vol. 54, Issue 5 https://doi.org/10.1107/s0907444998003254	journal	September 1998
Leucine-responsive regulatory protein and deoxyadenosine methylase control the phase variation and expression of the sfa and daa pili operons in Escherichia coli Woude, M. W.; Low, D. A. Molecular Microbiology, Vol. 11, Issue 4 https://doi.org/10.1111/j.1365-2958.1994.tb00340.x	journal	February 1994
Roles of DNA adenine methylation in host–pathogen interactions: mismatch repair, transcriptional regulation, and more Marinus, Martin G.; Casadesus, Josep FEMS Microbiology Reviews, Vol. 33, Issue 3 https://doi.org/10.1111/j.1574-6976.2008.00159.x	journal	May 2009
The SOS Regulatory Network Simmons, Lyle A.; Foti, James J.; Cohen, Susan E. EcoSal Plus, Vol. 3, Issue 1 https://doi.org/10.1128/ecosal.5.4.3	journal	July 2008
Replication of Vibrio cholerae Chromosome I in Escherichia coli: Dependence on Dam Methylation Koch, Birgit; Ma, Xiaofang; Løbner-Olesen, Anders Journal of Bacteriology, Vol. 192, Issue 15 https://doi.org/10.1128/jb.00311-10	journal	May 2010
Leucine-Responsive Regulatory Protein-Mediated Repression of clp (Encoding CS31A) Expression by l-Leucine and l-Alanine in Escherichia coli Crost, Cécile; Garrivier, Annie; Harel, Josée Journal of Bacteriology, Vol. 185, Issue 6 https://doi.org/10.1128/jb.185.6.1886-1894.2003	journal	March 2003
Analysis of Global Gene Expression and Double-Strand-Break Formation in DNA Adenine Methyltransferase- and Mismatch Repair-Deficient Escherichia coli Robbins-Manke, Jennifer L.; Zdraveski, Zoran Z.; Marinus, Martin Journal of Bacteriology, Vol. 187, Issue 20 https://doi.org/10.1128/jb.187.20.7027-7037.2005	journal	October 2005
Mismatch Repair in Replication Fidelity, Genetic Recombination, and Cancer Biology Modrich, Paul; Lahue, Robert Annual Review of Biochemistry, Vol. 65, Issue 1 https://doi.org/10.1146/annurev.bi.65.070196.000533	journal	June 1996
Base Flipping Roberts, Richard J.; Cheng, Xiaodong Annual Review of Biochemistry, Vol. 67, Issue 1 https://doi.org/10.1146/annurev.biochem.67.1.181	journal	June 1998
An Assessment of the Role of DNA Adenine Methyltransferase on Gene Expression Regulation in E coli Seshasayee, Aswin Sai Narain PLoS ONE, Vol. 2, Issue 3 https://doi.org/10.1371/journal.pone.0000273	journal	March 2007
PHENIX: a comprehensive Python-based system for macromolecular structure solution. Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Apollo - University of Cambridge Repository https://doi.org/10.17863/cam.45787	text	January 2010
Dam methylase accessibility as an instrument for analysis of mammalian chromatin structure Bulanenkova, Svetlana S.; Kozlova, Alena A.; Kotova, Elena S. Epigenetics, Vol. 6, Issue 9 https://doi.org/10.4161/epi.6.9.16476	journal	September 2011
Structure-guided Analysis Reveals Nine Sequence Motifs Conserved among DNA Amino-methyl-transferases, and Suggests a Catalytic Mechanism for these Enzymes Malone, Thomas; Blumenthal, Robert M.; Cheng, Xiaodong Journal of Molecular Biology, Vol. 253, Issue 4 https://doi.org/10.1006/jmbi.1995.0577	journal	November 1995
Viability of Escherichia coli K-12 DNA adenine methylase (dam) mutants requires increased expression of specific genes in the SOS regulon Peterson, Kenneth R.; Wertman, Kenneth F.; Mount, David W. Molecular and General Genetics MGG, Vol. 201, Issue 1 https://doi.org/10.1007/BF00397979	journal	September 1985
Preferential Site-specific Hemimethylation of GATC Sites in pBR322 DNA by Dam Methyltransferase from Escherichia coli Bergerat, A.; Kriebardis, A.; Guschlbauer, W. Journal of Biological Chemistry, Vol. 264, Issue 7 https://doi.org/10.1016/S0021-9258(19)84962-1	journal	March 1989
Rotation function calculations with GLRF program Tong, Liang; Rossmann, Michael G. Methods in Enzymology https://doi.org/10.1016/S0076-6879(97)76080-4	book	January 1997
Bacteriophage T2Dam and T4Dam DNA-[N6-adenine]-methyltransferases Hattman, Stanley; Malygin, Ernst G. Progress in Nucleic Acid Research and Molecular Biology Volume 77 https://doi.org/10.1016/S0079-6603(04)77003-8	book	January 2004
Timing and targeting: The biological functions of Dam methylation in E. coli Messer, Walter; Noyer-Weidner, Mario Cell, Vol. 54, Issue 6 https://doi.org/10.1016/S0092-8674(88)90911-7	journal	September 1988
Effects of global regulatory proteins and environmental conditionson fimbrial gene expression of F1651 and F1652 producedby Escherichia coli causing septicaemia in pigs Daigle, France; Forget, Céline; Martin, Christine Research in Microbiology, Vol. 151, Issue 7 https://doi.org/10.1016/S0923-2508(00)00226-6	journal	September 2000
Structure and Specificity of the Vertebrate Anti-Mutator Uracil-DNA Glycosylase SMUG1 Wibley, Jane E. A.; Waters, Timothy R.; Haushalter, Karl Molecular Cell, Vol. 11, Issue 6 https://doi.org/10.1016/S1097-2765(03)00235-1	journal	June 2003
The Mechanism by which DNA Adenine Methylase and PapI Activate the Pap Epigenetic Switch Hernday, Aaron D.; Braaten, Bruce A.; Low, David A. Molecular Cell, Vol. 12, Issue 4 https://doi.org/10.1016/S1097-2765(03)00383-6	journal	October 2003
Stopped-flow and Mutational Analysis of Base Flipping by the Escherichia coli Dam DNA-(adenine-N6)-methyltransferase Liebert, Kirsten; Hermann, Andrea; Schlickenrieder, Martina Journal of Molecular Biology, Vol. 341, Issue 2 https://doi.org/10.1016/j.jmb.2004.05.033	journal	August 2004
The Design Involved in PapI and Lrp Regulation of the pap Operon Kawamura, Tetsuya; Vartanian, Armand S.; Zhou, Hongjun Journal of Molecular Biology, Vol. 409, Issue 3 https://doi.org/10.1016/j.jmb.2011.01.058	journal	June 2011
Structure of the E. coli DNA Glycosylase AlkA Bound to the Ends of Duplex DNA: A System for the Structure Determination of Lesion-Containing DNA Bowman, Brian R.; Lee, Seongmin; Wang, Shuyu Structure, Vol. 16, Issue 8 https://doi.org/10.1016/j.str.2008.04.012	journal	August 2008
Chromatin profiling using targeted DNA adenine methyltransferase van Steensel, Bas; Delrow, Jeffrey; Henikoff, Steven Nature Genetics, Vol. 27, Issue 3 https://doi.org/10.1038/85871	journal	March 2001
The SRA domain of UHRF1 flips 5-methylcytosine out of the DNA helix Hashimoto, Hideharu; Horton, John R.; Zhang, Xing Nature, Vol. 455, Issue 7214 https://doi.org/10.1038/nature07280	journal	September 2008
Genome-wide mapping of methylated adenine residues in pathogenic Escherichia coli using single-molecule real-time sequencing Fang, Gang; Munera, Diana; Friedman, David I. Nature Biotechnology, Vol. 30, Issue 12 https://doi.org/10.1038/nbt.2432	journal	November 2012
Competitive interaction of the OxyR DNA-binding protein and the Dam methylase at the antigen 43 gene regulatory region in Escherichia coli: The antigen 43 phase switch in E. coli Waldron, Denise E.; Owen, Peter; Dorman, Charles J. Molecular Microbiology, Vol. 44, Issue 2 https://doi.org/10.1046/j.1365-2958.2002.02905.x	journal	April 2002
Genome-wide analysis of deoxyadenosine methyltransferase-mediated control of gene expression in Escherichia coli: Global expression analysis in dam mutant Oshima, Taku; Wada, Chieko; Kawagoe, Yuya Molecular Microbiology, Vol. 45, Issue 3 https://doi.org/10.1046/j.1365-2958.2002.03037.x	journal	July 2002
Protein−DNA binding in the absence of specific base-pair recognition Afek, Ariel; Schipper, Joshua L.; Horton, John Proceedings of the National Academy of Sciences, Vol. 111, Issue 48 https://doi.org/10.1073/pnas.1410569111	journal	October 2014
Proximal Recognition Sites Facilitate Intrasite Hopping by DNA Adenine Methyltransferase: MECHANISTIC EXPLORATION OF EPIGENETIC GENE REGULATION Pollak, Adam J.; Reich, Norbert O. Journal of Biological Chemistry, Vol. 287, Issue 27 https://doi.org/10.1074/jbc.M111.332502	journal	May 2012
Functional Characterization of Escherichia coli DNA Adenine Methyltransferase, a Novel Target for Antibiotics Mashhoon, Neda; Carroll, Michael; Pruss, Cynthia Journal of Biological Chemistry, Vol. 279, Issue 50 https://doi.org/10.1074/jbc.M408182200	journal	September 2004
Two Alternative Conformations of S -Adenosyl-L-homocysteine Bound to Escherichia coli DNA Adenine Methyltransferase and the Implication of Conformational Changes in Regulating the Catalytic Cycle Liebert, Kirsten; Horton, John R.; Chahar, Sanjay Journal of Biological Chemistry, Vol. 282, Issue 31 https://doi.org/10.1074/jbc.M700926200	journal	May 2007
The M.EcoRV DNA-(Adenine N ⁶ )-methyltransferase Uses DNA Bending for Recognition of an Expanded EcoDam Recognition Site Jurkowski, Tomasz P.; Anspach, Nils; Kulishova, Liliya Journal of Biological Chemistry, Vol. 282, Issue 51 https://doi.org/10.1074/jbc.M706933200	journal	October 2007
Modulation of Escherichia coli DNA Methyltransferase Activity by Biologically Derived GATC-flanking Sequences Coffin, Stephanie R.; Reich, Norbert O. Journal of Biological Chemistry, Vol. 283, Issue 29 https://doi.org/10.1074/jbc.M802502200	journal	May 2008
Carbon-Oxygen Hydrogen Bonding in Biological Structure and Function Horowitz, Scott; Trievel, Raymond C. Journal of Biological Chemistry, Vol. 287, Issue 50 https://doi.org/10.1074/jbc.R112.418574	journal	October 2012
Improved methods for building protein models in electron density maps and the location of errors in these models Jones, T. A.; Zou, J. Y.; Cowan, S. W. Acta Crystallographica Section A Foundations of Crystallography, Vol. 47, Issue 2, p. 110-119 https://doi.org/10.1107/S0108767390010224	journal	March 1991
Coot model-building tools for molecular graphics Emsley, Paul; Cowtan, Kevin Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132 https://doi.org/10.1107/S0907444904019158	journal	November 2004
PHENIX: a comprehensive Python-based system for macromolecular structure solution Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221 https://doi.org/10.1107/S0907444909052925	journal	January 2010
Features and development of Coot Emsley, P.; Lohkamp, B.; Scott, W. G. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4 https://doi.org/10.1107/S0907444910007493	journal	March 2010
Crystallography & NMR System: A New Software Suite for Macromolecular Structure Determination Brünger, A. T.; Adams, P. D.; Clore, G. M. Acta Crystallographica Section D Biological Crystallography, Vol. 54, Issue 5 https://doi.org/10.1107/S0907444998003254	journal	September 1998
Negative control of fae (K88) expression by the'global'regulator Lrp is modulated by the'local'regulator FaeA and affected by DNA methylation Huisman, Tako T.; Graaf, Frits K. Molecular Microbiology, Vol. 16, Issue 5 https://doi.org/10.1111/j.1365-2958.1995.tb02320.x	journal	June 1995
Fuse or die: how to survive the loss of Dam in V ibrio cholerae : Natural fusion of Val, Marie-Eve; Kennedy, Sean P.; Soler-Bistué, Alfonso J. Molecular Microbiology, Vol. 91, Issue 4 https://doi.org/10.1111/mmi.12483	journal	January 2014
Replication of Vibrio cholerae Chromosome I in Escherichia coli: Dependence on Dam Methylation Koch, Birgit; Ma, Xiaofang; Løbner-Olesen, Anders Journal of Bacteriology, Vol. 192, Issue 15 https://doi.org/10.1128/JB.00311-10	journal	May 2010
Leucine-Responsive Regulatory Protein-Mediated Repression of clp (Encoding CS31A) Expression by l-Leucine and l-Alanine in Escherichia coli Crost, Cécile; Garrivier, Annie; Harel, Josée Journal of Bacteriology, Vol. 185, Issue 6 https://doi.org/10.1128/JB.185.6.1886-1894.2003	journal	March 2003
Analysis of Global Gene Expression and Double-Strand-Break Formation in DNA Adenine Methyltransferase- and Mismatch Repair-Deficient Escherichia coli Robbins-Manke, Jennifer L.; Zdraveski, Zoran Z.; Marinus, Martin Journal of Bacteriology, Vol. 187, Issue 20 https://doi.org/10.1128/JB.187.20.7027-7037.2005	journal	October 2005

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