E-cadherin junction formation involves an active kinetic nucleation process

Biswas, Kabir H.; Hartman, Kevin L.; Yu, Cheng -han; Harrison, Oliver J.; Song, Hang; Smith, Adam W.; Huang, William Y. C.; Lin, Wan -Chen; Guo, Zhenhuan; Padmanabhan, Anup; Troyanovsky, Sergey M.; Dustin, Michael L.; Shapiro, Lawrence; Honig, Barry; Zaidel-Bar, Ronen; Groves, Jay T.

doi:10.1073/pnas.1513775112

Title: E-cadherin junction formation involves an active kinetic nucleation process

Journal Article · Wed Aug 19 00:00:00 EDT 2015 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.1513775112· OSTI ID:1221818

Biswas, Kabir H. ^[1]; Hartman, Kevin L. ^[2]; Yu, Cheng -han ^[1]; Harrison, Oliver J. ^[3]; Song, Hang ^[3]; Smith, Adam W. ^[4]; Huang, William Y. C. ^[4]; Lin, Wan -Chen ^[4]; Guo, Zhenhuan ^[1]; Padmanabhan, Anup ^[1]; Troyanovsky, Sergey M. ^[5]; Dustin, Michael L. ^[6]; Shapiro, Lawrence ^[7]; Honig, Barry ^[7]; Zaidel-Bar, Ronen ^[1]; Groves, Jay T. ^[8]

National Univ. of Singapore (Singapore)
National Univ. of Singapore (Singapore); Univ. of California, Berkeley, CA (United States)
Columiba Univ., New York, NY (United States)
Univ. of California, Berkeley, CA (United States)
Northwestern Univ., Chicago, IL (United States)
Univ. of Oxford, Headington (United Kingdom)
Columbia Univ., New York, NY (United States)
National Univ. of Singapore (Singapore); Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab., Berkeley, CA (United States)

Epithelial (E)-cadherin-mediated cell–cell junctions play important roles in the development and maintenance of tissue structure in multicellular organisms. E-cadherin adhesion is thus a key element of the cellular microenvironment that provides both mechanical and biochemical signaling inputs. Here, we report in vitro reconstitution of junction-like structures between native E-cadherin in living cells and the extracellular domain of E-cadherin in a supported membrane. Junction formation in this hybrid live cell-supported membrane configuration requires both active processes within the living cell and a supported membrane with low E-cad-ECD mobility. The hybrid junctions recruit α-catenin and exhibit remodeled cortical actin. Observations suggest that the initial stages of junction formation in this hybrid system depend on the trans but not the cis interactions between E-cadherin molecules, and proceed via a nucleation process in which protrusion and retraction of filopodia play a key role.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-05CH11231; MCB-1412472; CRP001-084; NRF-RF2009-RF001-074; 100262/Z/12/Z; PRF #100262/Z/12/Z; R01 GM062270; AR44016

OSTI ID:: 1221818

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, Issue 35; ISSN 0027-8424

Publisher:: National Academy of Sciences, Washington, DC (United States)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 65 works

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Title: E-cadherin junction formation involves an active kinetic nucleation process

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