Fluorescence dye tagging scheme for mercury quantification and speciation
Patent
·
OSTI ID:1221386
A fluorescent dye or fluorophore capable of forming complexes with mercury comprises 6,8-difluoro-7-hydroxy-2-oxo-2H-chromene-3-carboxylate amide, wherein the amide is formed by reacting the succinimidyl ester (Pacific Blue.TM.) with an amino acid containing a thiol group, such as cysteine or glutathione. Mercury complexes of the fluorophore fluoresce when excited by a UV or violet laser diode, and the detected intensity can be calibrated to quantify the concentration of mercury in a sample reacted with the fluorophore.
- Research Organization:
- Los Gatos Research, Mountain View, CA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- SC0004430
- Assignee:
- Los Gatos Research (Mountain View, CA)
- Patent Number(s):
- 9,139,550
- Application Number:
- 13/570,548
- OSTI ID:
- 1221386
- Country of Publication:
- United States
- Language:
- English
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