Radiation induction of the receptor tyrosine kinase gene Ptk-3 in normal rat astrocytes
- Univ. of Texas, Houston, TX (United States); and others
Radiation-induced gene expression was examined in rat astrocyte cultures using differential display of mRNA via reverse transcriptase-polymerase chain reaction. A 0.3-kb cDNA that was consistently observed in irradiated cultures but not in unirradiated cultures was cloned and sequenced. It was found to be identical to Ptk-3, a receptor tyrosine kinase gene identified recently. The protein encoded by Ptk-3 is a member of a novel class of receptor tyrosine kinases whose extracellular domain contains regions of homology with coagulation factors V and VIII and complement component C1. Northern blot analysis revealed that the expression of Ptk-3 was increased in rat astrocytes by 0.5 h after exposure to 10 Gy and remained at the same elevated level for at least 24 h. The maximum increase occurred after 5 Gy cloning studies indicated the presence of at least two Ptk-3 mRNA transcripts, which are probable the result of an alternative splicing mechanism. The short isoform lacks a 37 amino acid sequence in the glycine/proline-rich juxtamembrane region. The splicing pattern of the Ptk-3 gene was not altered by radiation. However, the ratios of the longer to the shorter mRNA transcripts differed between adult cortex, neonatal cortex and in vitro astrocyte cultures. 36 refs., 5 figs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 121701
- Journal Information:
- Radiation Research, Journal Name: Radiation Research Journal Issue: 1 Vol. 143; ISSN 0033-7587; ISSN RAREAE
- Country of Publication:
- United States
- Language:
- English
Similar Records
Mapping of the receptor protein-tyrosine kinase 10 to human chromosome 1q21-q23 and mouse chromosome 1H1-5 by fluorescence in situ hybridization
cDNA cloning, molecular characterization, and chromosomal localization of NET(EPHT2), a human EPH-related receptor protein-tyrosine kinase gene preferentially expressed in brain
Related Subjects
BASIC STUDIES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
AMINO ACID SEQUENCE
BIOLOGICAL RADIATION EFFECTS
CENTRAL NERVOUS SYSTEM
CLONING
DNA
GENE REPRESSORS
IONIZING RADIATIONS
MESSENGER-RNA
POLYMERASE CHAIN REACTION
PROLINE
RADIOINDUCTION
RATS
RNA POLYMERASES
TRANSCRIPTION
TYROSINE