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Title: Rapid detection of Ebola virus with a reagent-free, point-of-care biosensor

Abstract

Surface acoustic wave (SAW) sensors can rapidly detect Ebola antigens at the point-of-care without the need for added reagents, sample processing, or specialized personnel. This preliminary study demonstrates SAW biosensor detection of the Ebola virus in a concentration-dependent manner. The detection limit with this methodology is below the average level of viremia detected on the first day of symptoms by PCR. We observe a log-linear sensor response for highly fragmented Ebola viral particles, with a detection limit corresponding to 1.9 × 10⁴ PFU/mL prior to virus inactivation. We predict greatly improved sensitivity for intact, infectious Ebola virus. This point-of-care methodology has the potential to detect Ebola viremia prior to symptom onset, greatly enabling infection control and rapid treatment. This biosensor platform is powered by disposable AA batteries and can be rapidly adapted to detect other emerging diseases in austere conditions.

Authors:
 [1];  [1];  [1];  [2];  [1]
  1. Univ. of New Mexico School of Medicine, Albuquerque, NM (United States)
  2. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Publication Date:
Research Org.:
Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1213407
Grant/Contract Number:  
AC04-94AL85000
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Sensors
Additional Journal Information:
Journal Volume: 15; Journal Issue: 4; Journal ID: ISSN 1424-8220
Publisher:
MDPI AG
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; pathogens; infectious diseases; viruses; piezoelectric; biosensors; diagnosis; SAW; surface acoustic wave

Citation Formats

Baca, Justin T., Severns, Virginia, Lovato, Debbie, Branch, Darren W., and Larson, Richard S. Rapid detection of Ebola virus with a reagent-free, point-of-care biosensor. United States: N. p., 2015. Web. doi:10.3390/s150408605.
Baca, Justin T., Severns, Virginia, Lovato, Debbie, Branch, Darren W., & Larson, Richard S. Rapid detection of Ebola virus with a reagent-free, point-of-care biosensor. United States. doi:10.3390/s150408605.
Baca, Justin T., Severns, Virginia, Lovato, Debbie, Branch, Darren W., and Larson, Richard S. Tue . "Rapid detection of Ebola virus with a reagent-free, point-of-care biosensor". United States. doi:10.3390/s150408605. https://www.osti.gov/servlets/purl/1213407.
@article{osti_1213407,
title = {Rapid detection of Ebola virus with a reagent-free, point-of-care biosensor},
author = {Baca, Justin T. and Severns, Virginia and Lovato, Debbie and Branch, Darren W. and Larson, Richard S.},
abstractNote = {Surface acoustic wave (SAW) sensors can rapidly detect Ebola antigens at the point-of-care without the need for added reagents, sample processing, or specialized personnel. This preliminary study demonstrates SAW biosensor detection of the Ebola virus in a concentration-dependent manner. The detection limit with this methodology is below the average level of viremia detected on the first day of symptoms by PCR. We observe a log-linear sensor response for highly fragmented Ebola viral particles, with a detection limit corresponding to 1.9 × 10⁴ PFU/mL prior to virus inactivation. We predict greatly improved sensitivity for intact, infectious Ebola virus. This point-of-care methodology has the potential to detect Ebola viremia prior to symptom onset, greatly enabling infection control and rapid treatment. This biosensor platform is powered by disposable AA batteries and can be rapidly adapted to detect other emerging diseases in austere conditions.},
doi = {10.3390/s150408605},
journal = {Sensors},
issn = {1424-8220},
number = 4,
volume = 15,
place = {United States},
year = {2015},
month = {4}
}

Journal Article:
Free Publicly Available Full Text
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