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Title: Metabolomic profiling of the nectars of Aquilegia pubescens and A. Canadensis

Journal Article · · PLoS ONE
 [1];  [2];  [3];  [4];  [5];  [6]
  1. Cold Spring Harbor Lab., Cold Spring Harbor, NY (United States)
  2. Iowa State Univ., Ames, IA (United States). W.M. Keck Metabolomics Research Laboratory.
  3. Iowa State Univ., Ames, IA (United States). W.M. Keck Metabolomics Research Laboratory, and Dept. of Biochemistry, Biophysics, and Molecular Biology.
  4. Argonne National Lab. (ANL), Argonne, IL (United States). Mathematics and Computer Science Div.; The Univ. of Chicago, Chicago, IL (United States). Computation Institute.
  5. Cold Spring Harbor Lab., Cold Spring Harbor, NY (United States); USDA-ARS-NAA, Ithaca, NY (United States). Robert W. Holley Center.
  6. National Research Council of Italy (Italy)
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To date, variation in nectar chemistry of flowering plants has not been studied in detail. Such variation exerts considerable influence on pollinator–plant interactions, as well as on flower traits that play important roles in the selection of a plant for visitation by specific pollinators. Over the past 60 years the Aquilegia genus has been used as a key model for speciation studies. In this study, we defined the metabolomic profiles of flower samples of two Aquilegia species, A. Canadensis and A. pubescens. We identified a total of 75 metabolites that were classified into six main categories: organic acids, fatty acids, amino acids, esters, sugars, and unknowns. The mean abundances of 25 of these metabolites were significantly different between the two species, providing insights into interspecies variation in floral chemistry. Using the PlantSEED biochemistry database, we found that the majority of these metabolites are involved in biosynthetic pathways. Finally, we explored the annotated genome of A. coerulea, using the PlantSEED pipeline and reconstructed the metabolic network of Aquilegia. As a result, this network, which contains the metabolic pathways involved in generating the observed chemical variation, is now publicly available from the DOE Systems Biology Knowledge Base (KBase; http://kbase.us).

Research Organization:
Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Organization:
National Science Foundation (NSF); USDOE Office of Science (SC), Biological and Environmental Research (BER)
Grant/Contract Number:
AC02-06CH11357; AC02-98CH10886
OSTI ID:
1212415
Alternate ID(s):
OSTI ID: 1225046
Journal Information:
PLoS ONE, Vol. 10, Issue 5; ISSN 1932-6203
Publisher:
Public Library of ScienceCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 16 works
Citation information provided by
Web of Science

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Cited By (6)

Short versus prolonged freezing differentially impacts freeze – thaw injury in spinach leaves: mechanistic insights through metabolite profiling journal November 2019
Nectar sugars and amino acids in day- and night-flowering Nicotiana species are more strongly shaped by pollinators’ preferences than organic acids and inorganic ions journal May 2017
Metabolic Analysis of Medicinal Dendrobium officinale and Dendrobium huoshanense during Different Growth Years journal January 2016
Linden (Tilia cordata) associated bumble bee mortality: Metabolomic analysis of nectar and bee muscle journal July 2019
Isolation, Characterization, and Complete Genome Sequence of a Bradyrhizobium Strain Lb8 From Nodules of Peanut Utilizing Crack Entry Infection journal February 2020
Toward Mass Spectrometry Imaging in the Metabolomics Scale: Increasing Metabolic Coverage Through Multiple On-Tissue Chemical Modifications journal July 2019

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
metabolites
metabolic pathways
metabolic networks
organic acids
flowering plants
genomic databases
glucose metabolism
sucrose
esters