Enhancing Bottom-up and Top-down Proteomic Measurements with Ion Mobility Separations

Baker, Erin Shammel; Burnum-Johnson, Kristin E.; Ibrahim, Yehia M.; Orton, Daniel J.; Monroe, Matthew E.; Kelly, Ryan T.; Moore, Ronald J.; Zhang, Xing; Theberge, Roger; Costello, Catherine E.; Smith, Richard D.

doi:10.1002/pmic.201500048

Enhancing Bottom-up and Top-down Proteomic Measurements with Ion Mobility Separations

Journal Article · Fri Jul 03 00:00:00 EDT 2015 · Proteomics

DOI:https://doi.org/10.1002/pmic.201500048· OSTI ID:1212228

Baker, Erin Shammel ^[1]; Burnum-Johnson, Kristin E. ^[1]; Ibrahim, Yehia M. ^[1]; Orton, Daniel J. ^[1]; Monroe, Matthew E. ^[1]; Kelly, Ryan T. ^[1]; Moore, Ronald J. ^[1]; Zhang, Xing ^[1]; Theberge, Roger ^[2]; Costello, Catherine E. ^[2]; Smith, Richard D. ^[1]

Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Boston Univ. of Medicine, MA (United States)

Proteomic measurements with greater throughput, sensitivity and additional structural information enhance the in-depth characterization of complex mixtures and targeted studies with additional information and higher confidence. While liquid chromatography separation coupled with mass spectrometry (LC-MS) measurements have provided information on thousands of proteins in different sample types, the additional of another rapid separation stage providing structural information has many benefits for analyses. Technical advances in ion funnels and multiplexing have enabled ion mobility separations to be easily and effectively coupled with LC-MS proteomics to enhance the information content of measurements. Finally, herein, we report on applications illustrating increased sensitivity, throughput, and structural information by utilizing IMS-MS and LC-IMS-MS measurements for both bottom-up and top-down proteomics measurements.

View Accepted Manuscript (DOE)

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States), Environmental Molecular Sciences Laboratory (EMSL)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 1212228

Report Number(s):: PNNL-SA--107785; 48135; 48203; 400412000

Journal Information:: Proteomics, Journal Name: Proteomics Journal Issue: 16 Vol. 15; ISSN 1615-9853

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
Environmental Molecular Sciences Laboratory
Ion mobility separations
Ion mobility spectrometry
Mass spectrometry
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