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Title: Effect of Thiols, Zinc, and Redox Conditions on Hg Uptake in Shewanella oneidensis

Journal Article · · Environmental Science and Technology
 [1];  [1];  [1]
  1. Department of Geosciences, Princeton University, Princeton, New Jersey 08544, United States
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Mercury uptake in bacteria represents a key first step in the production and accumulation Of methylmercury in biota. Previous experiments with mercury methylating bacteria have shown that Hg uptake is enhanced by some thiols, in particular cysteine, and that it is an energy-dependent process through heavy Metal TA transporters. In this study, we examine Hg uptake in the nonmethylating facultative aerobe, Shewanella oneidensis, under both anaerobic and aerobic conditions. Our results demonstrate similar characteristics of the Hg uptake system to those of the Hg methylating strains: uptake is enhanced in the presence of some thiols but not others; uptake is energy dependent as evidenced by inhibition by a protonophore; and uptake is inhibited by high Zn(II) concentrations. Initial cellular uptake rates in S. oneidensis were remarkably similar under aerobic and fumarate-reducing conditions. In conclusion, these data support a similar Hg(II) uptake mechanism within the proteobacteria of accidental Hg(II) transport through heavy metal transporters with similar rates of uptake but differences in the ability to take up Hg bound to different thiols.

Research Organization:
Rutgers Univ., New Brunswick, NJ (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
Grant/Contract Number:
SC0006849
OSTI ID:
1209997
Alternate ID(s):
OSTI ID: 1441261
Journal Information:
Environmental Science and Technology, Journal Name: Environmental Science and Technology Vol. 49 Journal Issue: 12; ISSN 0013-936X
Publisher:
American Chemical SocietyCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 35 works
Citation information provided by
Web of Science

References (26)

Differential protein expression in the metal-reducing bacteriumGeobacter sulfurreducens strain PCA grown with fumarate or ferric citrate journal January 2006
Mercury Methylation by Dissimilatory Iron-Reducing Bacteria journal October 2006
Global Transcriptome Analysis of Shewanella oneidensis MR-1 Exposed to Different Terminal Electron Acceptors journal September 2005
Effect of Inorganic and Organic Ligands on the Bioavailability of Methylmercury as Determined by Using a mer-lux Bioreporter journal August 2012
The impact of ionic mercury on antioxidant defenses in two mercury-sensitive anaerobic bacteria journal October 2013
Effect of Divalent Metals on Hg(II) Uptake and Methylation by Bacteria journal February 2014
A thermophilic bacterial origin and subsequent constraints by redox, light and salinity on the evolution of the microbial mercuric reductase: The natural history of the mercuric reductase journal November 2010
Evidence for facilitated uptake of Hg(II) by Vibrio anguillarum and Escherichia coli under anaerobic and aerobic conditions journal July 2002
High methylation rates of mercury bound to cysteine by Geobacter sulfurreducens journal January 2009
Sequestration of Hg 2+ by Some Biologically Important Thiols journal December 2011
Syntrophs Dominate Sequences Associated with the Mercury Methylation-Related Gene hgcA in the Water Conservation Areas of the Florida Everglades journal August 2014
Mercury Methylation by the Methanogen Methanospirillum hungatei journal August 2013
Unexpected Effects of Gene Deletion on Interactions of Mercury with the Methylation-Deficient Mutant Δ hgcAB journal April 2014
Analysis of the Microbial Community Structure by Monitoring an Hg Methylation Gene (hgcA) in Paddy Soils along an Hg Gradient journal February 2014
Mercury Methylation by Novel Microorganisms from New Environments journal September 2013
Bacterial mercury resistance from atoms to ecosystems journal June 2003
Effect of pH on Intracellular Accumulation of Trace Concentrations of Hg(II) in Escherichia coli under Anaerobic Conditions, as Measured Using a mer-lux Bioreporter journal February 2008
Sulfate-Reducing Bacterium Desulfovibrio desulfuricans ND132 as a Model for Understanding Bacterial Mercury Methylation journal April 2011
Hg( ii ) bacterial biouptake: the role of anthropogenic and biogenic ligands present in solution and spectroscopic evidence of ligand exchange reactions at the cell surface journal January 2014
Detection of a key Hg methylation gene, hgcA , in wetland soils : Detection of the Hg methylation gene, hgcA, in soils journal January 2014
Active transport, substrate specificity, and methylation of Hg(II) in anaerobic bacteria journal May 2011
Bacterial Manganese Reduction and Growth with Manganese Oxide as the Sole Electron Acceptor journal June 1988
Determination of volatile mercury species at the picogram level by low-temperature gas chromatography with cold-vapour atomic fluorescence detection journal January 1988
The Genetic Basis for Bacterial Mercury Methylation journal February 2013
Metal Chelates of Some Sulfur-containing Amino Acids * journal June 1964
Nuclear magnetic resonance studies of the solution chemistry of metal complexes. 25. Hg(thiol)3 complexes and HG(II)-thiol ligand exchange kinetics journal September 1988

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