Evaluation of Novel Design Strategies for Developing Zinc Finger Nucleases Tools for Treating Human Diseases

Bach, Christian; Sherman, William; Pallis, Jani; Patra, Prabir; Bajwa, Hassan

doi:10.1155/2014/970595

Title: Evaluation of Novel Design Strategies for Developing Zinc Finger Nucleases Tools for Treating Human Diseases

Journal Article · Sun Apr 06 00:00:00 EDT 2014 · Biotechnology Research International

DOI:https://doi.org/10.1155/2014/970595· OSTI ID:1209764

Bach, Christian ^[1]; Sherman, William ^[2]; Pallis, Jani ^[3];

^[1]; Bajwa, Hassan ^[4]

University of Bridgeport, Biomedical Engineering, 221 University Avenue, Bridgeport, CT 06604, USA
Physics Faculty, BHSEC Queens, 30-20 Thomson Avenue, Long Island City, NY 11101, USA
University of Bridgeport, Mechanical Engineering, 221 University Avenue, Bridgeport, CT 06604, USA
University of Bridgeport, Electrical Engineering, 221 University Avenue, Bridgeport, CT 06604, USA

Zinc finger nucleases (ZFNs) are associated with cell death and apoptosis by binding at countless undesired locations. This cytotoxicity is associated with the binding ability of engineered zinc finger domains to bind dissimilar DNA sequences with high affinity. In general, binding preferences of transcription factors are associated with significant degenerated diversity and complexity which convolutes the design and engineering of precise DNA binding domains. Evolutionary success of natural zinc finger proteins, however, evinces that nature created specific evolutionary traits and strategies, such as modularity and rank-specific recognition to cope with binding complexity that are critical for creating clinical viable tools to precisely modify the human genome. Our findings indicate preservation of general modularity and significant alteration of the rank-specific binding preferences of the three-finger binding domain of transcription factor SP1 when exchanging amino acids in the 2nd finger.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-98CH10886

OSTI ID:: 1209764

Alternate ID(s):: OSTI ID: 1629932

Journal Information:: Biotechnology Research International, Journal Name: Biotechnology Research International Vol. 2014; ISSN 2090-3138

Publisher:: Hindawi Publishing CorporationCopyright Statement

Country of Publication:: Egypt

Language:: English

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