MALDI-Mass Spectrometric Imaging Revealing Hypoxia-Driven Lipids and Proteins in a Breast Tumor Model

Jiang, Lu; Chughtai, Kamila; Purvine, Samuel O.; Bhujwalla, Zaver M.; Raman, Venu; Paša-Tolić, Ljiljana; Heeren, Ron M.  A.; Glunde, Kristine

doi:10.1021/ac504503x

MALDI-Mass Spectrometric Imaging Revealing Hypoxia-Driven Lipids and Proteins in a Breast Tumor Model

Journal Article · Wed May 20 00:00:00 EDT 2015 · Analytical Chemistry

DOI:https://doi.org/10.1021/ac504503x· OSTI ID:1203872

Jiang, Lu ^[1]; Chughtai, Kamila ^[2]; Purvine, Samuel O. ^[3]; Bhujwalla, Zaver M. ^[4]; Raman, Venu ^[4]; Paša-Tolić, Ljiljana ^[3]; Heeren, Ron M. A. ^[5]; Glunde, Kristine ^[4]

Johns Hopkins Univ., Baltimore, MD (United States). Division of Cancer Imaging Research, Russell H. Morgan Dept. of Radiology and Radiological Science
Fundamental Research on Matter (FOM) AMOLF, Amsterdam (The Netherlands)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
Johns Hopkins Univ., Baltimore, MD (United States). Division of Cancer Imaging Research, Russell H. Morgan Dept. of Radiology and Radiological Science; Johns Hopkins Univ., Baltimore, MD (United States). School of Medicine, Sidney Kimmel Comprehensive Cancer Center
Fundamental Research on Matter (FOM) AMOLF, Amsterdam (The Netherlands); Maastricht MultiModal Molecular Imaging Institute (M4I), Maastricht (The Netherlands)

Hypoxic areas are a common feature of rapidly growing malignant tumors and their metastases, and are typically spatially heterogeneous. Hypoxia has a strong impact on tumor cell biology and contributes to tumor progression in multiple ways. To date, only a few molecular key players in tumor hypoxia, such as for example hypoxia-inducible factor-1 (HIF-1), have been discovered. The distribution of biomolecules is frequently heterogeneous in the tumor volume, and may be driven by hypoxia and HIF-1α. Understanding the spatially heterogeneous hypoxic response of tumors is critical. Mass spectrometric imaging (MSI) provides a unique way of imaging biomolecular distributions in tissue sections with high spectral and spatial resolution. In this paper, breast tumor xenografts grown from MDA-MB-231-HRE-tdTomato cells, with a red fluorescent tdTomato protein construct under the control of a hypoxia response element (HRE)-containing promoter driven by HIF-1α, were used to detect the spatial distribution of hypoxic regions. We elucidated the 3D spatial relationship between hypoxic regions and the localization of small molecules, metabolites, lipids, and proteins by using principal component analysis – linear discriminant analysis (PCA-LDA) on 3D rendered MSI volume data from MDA-MB-231-HRE-tdTomato breast tumor xenografts. In this study we identified hypoxia-regulated proteins active in several distinct pathways such as glucose metabolism, regulation of actin cytoskeleton, protein folding, translation/ribosome, splicesome, the PI3K-Akt signaling pathway, hemoglobin chaperone, protein processing in endoplasmic reticulum, detoxification of reactive oxygen species, aurora B signaling/apoptotic execution phase, the RAS signaling pathway, the FAS signaling pathway/caspase cascade in apoptosis and telomere stress induced senescence. In parallel we also identified co-localization of hypoxic regions and various lipid species such as PC(16:0/18:1), PC(16:0/18:2), PC(18:0/18:1), PC(18:1/18:1), PC(18:1/18:2), PC(16:1/18:4), PC(18:0/20:3), PC(16:0/22:1), among others. Lastly, our findings shed light on the biomolecular composition of hypoxic tumor regions, which may be responsible for a given tumor’s resistance to radiation or chemotherapy.

View Accepted Manuscript (DOE)

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 1203872

Report Number(s):: PNNL-SA--110776; 40892; KP1704020

Journal Information:: Analytical Chemistry, Journal Name: Analytical Chemistry Journal Issue: 12 Vol. 87; ISSN 0003-2700

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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Additional file 1 of Immune and stromal scoring system associated with tumor microenvironment and prognosis: a gene-based multi-cancer analysis Zeng, Zihang; Li, Jiali; Zhang, Jianguo figshare https://doi.org/10.6084/m9.figshare.15103062.v1	image	January 2021
Additional file 2 of Immune and stromal scoring system associated with tumor microenvironment and prognosis: a gene-based multi-cancer analysis Zeng, Zihang; Li, Jiali; Zhang, Jianguo figshare https://doi.org/10.6084/m9.figshare.15103065.v1	image	January 2021
Additional file 3 of Immune and stromal scoring system associated with tumor microenvironment and prognosis: a gene-based multi-cancer analysis Zeng, Zihang; Li, Jiali; Zhang, Jianguo figshare https://doi.org/10.6084/m9.figshare.15103068.v1	image	January 2021
Additional file 4 of Immune and stromal scoring system associated with tumor microenvironment and prognosis: a gene-based multi-cancer analysis Zeng, Zihang; Li, Jiali; Zhang, Jianguo figshare https://doi.org/10.6084/m9.figshare.15103071	image	January 2021
Additional file 5 of Immune and stromal scoring system associated with tumor microenvironment and prognosis: a gene-based multi-cancer analysis Zeng, Zihang; Li, Jiali; Zhang, Jianguo figshare https://doi.org/10.6084/m9.figshare.15103074	image	January 2021
Additional file 6 of Immune and stromal scoring system associated with tumor microenvironment and prognosis: a gene-based multi-cancer analysis Zeng, Zihang; Li, Jiali; Zhang, Jianguo figshare https://doi.org/10.6084/m9.figshare.15103077.v1	image	January 2021
Additional file 7 of Immune and stromal scoring system associated with tumor microenvironment and prognosis: a gene-based multi-cancer analysis Zeng, Zihang; Li, Jiali; Zhang, Jianguo figshare https://doi.org/10.6084/m9.figshare.15103080	image	January 2021
Additional file 8 of Immune and stromal scoring system associated with tumor microenvironment and prognosis: a gene-based multi-cancer analysis Zeng, Zihang; Li, Jiali; Zhang, Jianguo figshare https://doi.org/10.6084/m9.figshare.15103083.v1	image	January 2021
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60 APPLIED LIFE SCIENCES
Environmental Molecular Sciences Laboratory
breast cancer
hypoxia
phosphatidylcholine
lipid
peptide
mass spectrometric imaging
protein

MALDI-Mass Spectrometric Imaging Revealing Hypoxia-Driven Lipids and Proteins in a Breast Tumor Model

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