Crystallization of lysozyme with (R)-, (S)- and (RS)-2-methyl-2,4-pentanediol

Stauber, Mark; Jakoncic, Jean; Berger, Jacob; Karp, Jerome M.; Axelbaum, Ariel; Sastow, Dahniel; Buldyrev, Sergey V.; Hrnjez, Bruce J.; Asherie, Neer

doi:10.1107/S1399004714025061

Title: Crystallization of lysozyme with (R)-, (S)- and (RS)-2-methyl-2,4-pentanediol

Journal Article · Sun Mar 01 00:00:00 EST 2015 · Acta Crystallographica. Section D: Biological Crystallography (Online)

DOI:https://doi.org/10.1107/S1399004714025061· OSTI ID:1193215

Stauber, Mark ^[1]; Jakoncic, Jean ^[2]; Berger, Jacob ^[3]; Karp, Jerome M. ^[4]; Axelbaum, Ariel ^[5]; Sastow, Dahniel ^[5]; Buldyrev, Sergey V. ^[5]; Hrnjez, Bruce J. ^[6]; Asherie, Neer ^[5]

Yeshiva Univ., New York, NY (United States); Stanford Univ., Stanford, CA (United States)
Brookhaven National Lab. (BNL), Upton, NY (United States)
Yeshiva Univ., New York, NY (United States); Univ. of Pennsylvania, Philadelphia, PA (United States)
Yeshiva Univ., New York, NY (United States); Yeshiva Univ., Bronx, NY (United States)
Yeshiva Univ., New York, NY (United States)
Collegiate School, New York, NY (United States)

Chiral control of crystallization has ample precedent in the small-molecule world, but relatively little is known about the role of chirality in protein crystallization. In this study, lysozyme was crystallized in the presence of the chiral additive 2-methyl-2,4-pentanediol (MPD) separately using the R and S enantiomers as well as with a racemic RS mixture. Crystals grown with (R)-MPD had the most order and produced the highest resolution protein structures. This result is consistent with the observation that in the crystals grown with (R)-MPD and (RS)-MPD the crystal contacts are made by (R)-MPD, demonstrating that there is preferential interaction between lysozyme and this enantiomer. These findings suggest that chiral interactions are important in protein crystallization.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: SC00112704

OSTI ID:: 1193215

Report Number(s):: BNL-108006-2015-JA; ABCRE6; R&D Project: LS001

Journal Information:: Acta Crystallographica. Section D: Biological Crystallography (Online), Vol. 71, Issue 3; ISSN 1399-0047

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 4 works

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