[{sup 3}H]-2,3,7,8-TCDD uptake and elimination kinetics of medaka (Oryzias latipes)

Journal Article · Sun Oct 01 04:00:00 EDT 1995 · Environmental Toxicology and Chemistry

DOI:https://doi.org/10.1897/1552-8618(1995)14[1735:HUAEKO]2.0.CO;2· OSTI ID:118644

Schmieder, P; Lothenbach, D; Tietge, J; Erickson, R; Johnson, R ^[1]

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1. Environmental Protection Agency, Duluth, MI (United States). Environmental Research Lab.

Uptake and elimination rate constants for [{sup 3}H]-2,3,7,8-tetrachlorodibenzo-p-dioxin ([{sup 3}H]TCDD) were estimated by exposing medaka (Oryzias latipes) to [{sup 3}H]TCDD in flowing water with no solvent carriers. Uptake was determined from body-burdens measured on exposure days 0, 2, 4, 6, 10, and 12. Elimination was quantified for whole fish after 28, 90, and 175 d in uncontaminated water. Medaka accumulated [{sup 3}H]TCDD at a rapid rate, achieving residues 24,000 times the water concentration after 12 d, with no indication of approach to steady state. After 6 months in uncontaminated water, the pg TCDD/g decreased by 69%, much of the decrease due to growth dilution as evidenced by only a 47% decrease in the pg TCDD/fish. Uptake and elimination rate constant (2,300 ml/g/d and 0.0045/d, respectively) were estimated by fitting a one-compartment, linear, mass-balance model to the data, adjusting for growth rate. The experimental design, including solvent-free delivery of [{sup 3}H]TCDD, exposure at concentrations below maximum water solubility, and measurement of fish growth and lipid content during a 6-month elimination phase, resulted in a predicted steady-state bioconcentration factor (BCF) for medaka of 510,000, a number considerably higher than previously reported for dioxin BCFs. Kinetic parameters were used to successfully predict TCDD BCF in medaka exposed independently.

• Sponsoring Organization: USDOE
• OSTI ID: 118644
• Journal Information: Environmental Toxicology and Chemistry, Journal Name: Environmental Toxicology and Chemistry Journal Issue: 10 Vol. 14; ISSN ETOCDK; ISSN 0730-7268
• Country of Publication: United States
• Language: English

Related Subjects

56 BIOLOGY AND MEDICINE
APPLIED STUDIES
ANIMAL GROWTH
BODY BURDEN
DIOXIN
FISHES
FORECASTING
MATHEMATICAL MODELS
METABOLISM
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UPTAKE