Genome sequencing of the Trichoderma reesei QM9136 mutant identifies a truncation of the transcriptional regulator XYR1 as the cause for its cellulase-negative phenotype

Lichius, Alexander; Bidard, Frédérique; Buchholz, Franziska; Le Crom, Stéphane; Martin, Joel; Schackwitz, Wendy; Austerlitz, Tina; Grigoriev, Igor V; Baker, Scott E; Margeot, Antoine; Seiboth, Bernhard; Kubicek, Christian P

doi:10.1186/s12864-015-1526-0

Genome sequencing of the Trichoderma reesei QM9136 mutant identifies a truncation of the transcriptional regulator XYR1 as the cause for its cellulase-negative phenotype

Journal Article · Mon Apr 20 00:00:00 EDT 2015 · BMC Genomics

DOI:https://doi.org/10.1186/s12864-015-1526-0· OSTI ID:1185053

Lichius, Alexander ^[1]; Bidard, Frédérique ^[2]; Buchholz, Franziska ^[1]; Le Crom, Stéphane ^[3]; Martin, Joel ^[4]; Schackwitz, Wendy ^[4]; Austerlitz, Tina ^[1]; Grigoriev, Igor V ^[4]; Baker, Scott E ^[5]; Margeot, Antoine ^[2]; Seiboth, Bernhard ^[1]; Kubicek, Christian P ^[1]

Vienna Univ. of Technology, Vienna (Austria)
IFP Energies Nouvelles, Rueil-Malmaison (France)
Sorbonne Univ., Paris (France)
Joint Genome Inst., Walnut Creek, CA (United States). US Dept. of Energy.
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Trichoderma reesei is the main industrial source of cellulases and hemicellulases required for the hydrolysis of biomass to simple sugars, which can then be used in the production of biofuels and biorefineries. The highly productive strains in use today were generated by classical mutagenesis. As byproducts of this procedure, mutants were generated that turned out to be unable to produce cellulases. In order to identify the mutations responsible for this inability, we sequenced the genome of one of these strains, QM9136, and compared it to that of its progenitor T. reesei QM6a.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). Genomics Div.; Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: Austrian Science Fund; USDOE; USDOE Office of Science (SC). Joint Genome Institute

Grant/Contract Number:: AC02-05CH11231; AC05-76RL01830

OSTI ID:: 1185053

Alternate ID(s):: OSTI ID: 1208746
OSTI ID: 1242698

Report Number(s):: LBNL--177776; PNNL-SA--110701; PII: 1526

Journal Information:: BMC Genomics, Journal Name: BMC Genomics Journal Issue: 1 Vol. 16; ISSN 1471-2164

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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Genome sequencing of the Trichoderma reesei QM9136 mutant identifies a truncation of the transcriptional regulator XYR1 as the cause for its cellulase-negative phenotype

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