Activity-Based Protein Profiling of Microbes

Journal Article · Sun Feb 01 04:00:00 EST 2015 · Current Opinion in Chemical Biology, 24:139-144

DOI:https://doi.org/10.1016/j.cbpa.2014.10.022· OSTI ID:1184951

Sadler, Natalie C.; Wright, Aaron T.

Activity-Based Protein Profiling (ABPP) in conjunction with multimodal characterization techniques has yielded impactful findings in microbiology, particularly in pathogen, bioenergy, drug discovery, and environmental research. Using small molecule chemical probes that react irreversibly with specific proteins or protein families in complex systems has provided insights in enzyme functions in central metabolic pathways, drug-protein interactions, and regulatory protein redox, for systems ranging from photoautotrophic cyanobacteria to mycobacteria, and combining live cell or cell extract ABPP with proteomics, molecular biology, modeling, and other techniques has greatly expanded our understanding of these systems. New opportunities for application of ABPP to microbial systems include: enhancing protein annotation, characterizing protein activities in myriad environments, and reveal signal transduction and regulatory mechanisms in microbial systems.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 1184951
• Report Number(s): PNNL-SA-105009; 47418; 35199; KP1601010
• Journal Information: Current Opinion in Chemical Biology, 24:139-144, Journal Name: Current Opinion in Chemical Biology, 24:139-144
• Country of Publication: United States
• Language: English

Related Subjects

Environmental Molecular Sciences Laboratory