An ancient protein-DNA interaction underlying metazoan sex determination

Murphy, Mark W.; Lee, John K.; Rojo, Sandra; Gearhart, Micah D.; Kurahashi, Kayo; Banerjee, Surajit; Loeuille, Guy-André; Bashamboo, Anu; McElreavey, Kenneth; Zarkower, David; Aihara, Hideki; Bardwell, Vivian J.

doi:10.1038/nsmb.3032

Title: An ancient protein-DNA interaction underlying metazoan sex determination

Journal Article · Mon May 25 00:00:00 EDT 2015 · Nature Structural & Molecular Biology

DOI:https://doi.org/10.1038/nsmb.3032· OSTI ID:1184236

Murphy, Mark W. ^[1]; Lee, John K. ^[2]; Rojo, Sandra ^[3]; Gearhart, Micah D. ^[1]; Kurahashi, Kayo ^[2]; Banerjee, Surajit ^[4]; Loeuille, Guy-André ^[5]; Bashamboo, Anu ^[3]; McElreavey, Kenneth ^[3]; Zarkower, David ^[6]; Aihara, Hideki ^[7]; Bardwell, Vivian J. ^[6]

Univ. of Minnesota, Minneapolis, MN (United States). Dept. of Genetics, Cell Biology, and Development
Univ. of Minnesota, Minneapolis, MN (United States). Dept. of Biochemistry, Molecular Biology and Biophysics
Pasteur Inst., Paris (France). Unit of Human Developmental Genetics
Cornell Univ., Argonne, IL (United States). Northeastern Collaborative Access Team
Hospital Center of Dunkirk (France). Pediatric Ward
Univ. of Minnesota, Minneapolis, MN (United States). Dept. of Genetics, Cell Biology, and Development. Masonic Cancer Center. Developmental Biology Center
Univ. of Minnesota, Minneapolis, MN (United States). Dept. of Biochemistry, Molecular Biology and Biophysics. Masonic Cancer Center

DMRT transcription factors are deeply conserved regulators of metazoan sexual development. They share the DM DNA-binding domain, a unique intertwined double zinc-binding module followed by a C-terminal recognition helix, which binds a pseudopalindromic target DNA. In this paper, we show that DMRT proteins use a unique binding interaction, inserting two adjacent antiparallel recognition helices into a widened DNA major groove to make base-specific contacts. Versatility in how specific base contacts are made allows human DMRT1 to use multiple DNA binding modes (tetramer, trimer and dimer). Chromatin immunoprecipitation with exonuclease treatment (ChIP-exo) indicates that multiple DNA binding modes also are used in vivo. We show that mutations affecting residues crucial for DNA recognition are associated with an intersex phenotype in flies and with male-to-female sex reversal in humans. Finally, our results illuminate an ancient molecular interaction underlying much of metazoan sexual development.

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Research Organization:: Univ. of Minnesota, Minneapolis, MN (United States)

Sponsoring Organization:: USDOE Office of Science (SC); National Inst. of Health (NIH) (United States); European Cooperation in Science and Technology (COST) (Belgium)

Contributing Organization:: Pasteur Inst., Paris (France); Cornell Univ., Argonne, IL (United States); Hospital Center of Dunkirk (France)

Grant/Contract Number:: AC02-06CH11357; P41 GM103403; GM59152; GM50399; AI087098; GM095558; BM1303

OSTI ID:: 1184236

Journal Information:: Nature Structural & Molecular Biology, Vol. 22, Issue 6; ISSN 1545-9993

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 74 works

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59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
DMRT protein
DM domain
sex determination
DMRT1
DNA-binding proteins
transcription factors

Title: An ancient protein-DNA interaction underlying metazoan sex determination

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References (55)

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