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Atomic-resolution 3D structure of amyloid β fibrils: The Osaka mutation

Journal Article · · Angewandte Chemie (International Edition)
 [1];  [2];  [3];  [1];  [3];  [1];  [4];  [5];  [3];  [1]
  1. ETH Zurich, Wolfgang-Pauli-Strasse. Zurich (Switzerland)
  2. Brookhaven National Lab. (BNL), Upton, NY (United States)
  3. ETH Zurich, Zurich (Switzerland)
  4. Goethe Univ., Frankfurt (Germany)
  5. Univ. de Lyon, Lyon (France)
+ Show Author Affiliations

Despite its central importance for understanding the molecular basis of Alzheimer's disease (AD), high-resolution structural information on amyloid β-peptide (Aβ) fibrils, which are intimately linked with AD, is scarce. We report an atomic-resolution fibril structure of the Aβ 1-40 peptide with the Osaka mutation (E22Δ), associated with early-onset AD. The structure, which differs substantially from all previously proposed models, is based on a large number of unambiguous intra- and intermolecular solid-state NMR distance restraints

Research Organization:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI ID:
1183283
Report Number(s):
BNL--107758-2015-JA; KP1501010
Journal Information:
Angewandte Chemie (International Edition), Journal Name: Angewandte Chemie (International Edition) Journal Issue: 1 Vol. 54; ISSN 1433-7851
Publisher:
WileyCopyright Statement
Country of Publication:
United States
Language:
English

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Structure of amyloid-β (20-34) with Alzheimer’s-associated isomerization at Asp23 reveals a distinct protofilament interface journal July 2019
Cryo-EM structure and polymorphism of Aβ amyloid fibrils purified from Alzheimer’s brain tissue journal October 2019
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High-resolution NMR characterization of low abundance oligomers of amyloid-β without purification journal July 2015
Fibrils of Truncated Pyroglutamyl-Modified Aβ Peptide Exhibit a Similar Structure as Wildtype Mature Aβ Fibrils journal September 2016
Structural heterogeneity and intersubject variability of Aβ in familial and sporadic Alzheimer’s disease journal January 2018
Out-of-Register Aβ42 Assemblies as Models for Neurotoxic Oligomers and Fibrils journal September 2017
Conformational Dynamics and Stability of U-Shaped and S-Shaped Amyloid β Assemblies journal February 2018
APP Osaka Mutation in Familial Alzheimer’s Disease—Its Discovery, Phenotypes, and Mechanism of Recessive Inheritance journal February 2020
Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy journal September 2017
Alternative salt bridge formation in Aβ—a hallmark of early-onset Alzheimer's disease? text January 2015
Towards the design of anti-amyloid short peptide helices journal January 2018
A long‐lived Aβ oligomer resistant to fibrillization journal January 2018
Rational Structure‐Based Design of Fluorescent Probes for Amyloid Folds journal February 2019
Distal amyloid β-protein fragments template amyloid assembly: Distal Amyloid Β-Protein Fragments Template Amyloid Assembly journal February 2018
Stability of Aβ‐fibril fragments in the presence of fatty acids journal September 2019
Combined automated NOE assignment and structure calculation with CYANA journal March 2015
Direct amide 15N to 13C transfers for solid-state assignment experiments in deuterated proteins journal September 2018
CONFINE-MAS: a magic-angle spinning NMR probe that confines the sample in case of a rotor explosion journal December 2018
Structural variation in amyloid-β fibrils from Alzheimer's disease clinical subtypes journal January 2017
The chaperone αB-crystallin uses different interfaces to capture an amorphous and an amyloid client journal October 2015
Solid-state NMR structure of a pathogenic fibril of full-length human α-synuclein journal March 2016
The structure of a β2-microglobulin fibril suggests a molecular basis for its amyloid polymorphism journal October 2018
A new era for understanding amyloid structures and disease journal September 2018
A near atomic-scale view at the composition of amyloid-beta fibrils by atom probe tomography journal December 2018
Conservation of the Amyloid Interactome Across Diverse Fibrillar Structures journal March 2019
Amyloid-β adopts a conserved, partially folded structure upon binding to zwitterionic lipid bilayers prior to amyloid formation journal January 2016
Secondary nucleation in amyloid formation journal January 2018
Total chemical synthesis and biophysical properties of a designed soluble 24 kDa amyloid analogue journal January 2018
An amplification label of core–shell CdSe@CdS QD sensitized GO for a signal-on photoelectrochemical immunosensor for amyloid β-protein journal January 2019
Conversion between parallel and antiparallel β-sheets in wild-type and Iowa mutant Aβ40 fibrils journal January 2018
Peptide dimer structure in an Aβ(1–42) fibril visualized with cryo-EM journal September 2015
Molecular structure of monomorphic peptide fibrils within a kinetically trapped hydrogel network journal July 2015
Cryo-EM reveals the steric zipper structure of a light chain-derived amyloid fibril journal May 2016
Atomic-resolution structure of a disease-relevant Aβ(1–42) amyloid fibril journal July 2016
Combining DNP NMR with segmental and specific labeling to study a yeast prion protein strain that is not parallel in-register journal March 2017
Truncated Amyloid-β (11–40/42) from Alzheimer Disease Binds Cu 2+ with a Femtomolar Affinity and Influences Fiber Assembly journal September 2015
Mechanical and thermodynamic properties of Aβ42 , Aβ40 and α-synuclein fibrils: A coarse-grained method to complement experimental studies posted_content January 2019
Stability of Aβ-fibril fragments in the presence of fatty acids journal April 2019
Two new polymorphic structures of alpha-synuclein solved by cryo-electron microscopy posted_content November 2019
Significantly different contact patterns between Aβ40 and Aβ42 monomers involving the N‐terminal region journal August 2019
Amyloid fibril polymorphism: a challenge for molecular imaging and therapy journal February 2018
Fibril structure of amyloid-β(1–42) by cryo–electron microscopy journal September 2017
New Mechanism of Amyloid Fibril Formation journal May 2019
The Role of Structural Polymorphism in Driving the Mechanical Performance of the Alzheimer's Beta Amyloid Fibrils journal April 2019
Different Synergy in Amyloids and Biologically Active Forms of Proteins journal September 2019
Alternative Structures of α-Synuclein journal January 2020
Mechanical and thermodynamic properties of Aβ 42 , Aβ 40 , and α-synuclein fibrils: a coarse-grained method to complement experimental studies journal January 2019
Direct amide N-15 to C-13 transfers for solid-state assignment experiments in deuterated proteins text January 2018
Two new polymorphic structures of human full-length alpha-synuclein fibrils solved by cryo-electron microscopy text January 2019
Two new polymorphic structures of human full-length alpha-synuclein fibrils solved by cryo-electron microscopy journal December 2019

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