Systems-Level Synthetic Biology for Advanced Biofuel Production

Technical Report · Sun Mar 01 04:00:00 EST 2015

DOI:https://doi.org/10.2172/1177597· OSTI ID:1177597

Ruffing, Anne ^[1]; Jensen, Travis J. ^[1]; Strickland, Lucas Marshall ^[1]; Meserole, Stephen ^[1]; Tallant, David ^[1]

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1. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

Cyanobacteria have been shown to be capable of producing a variety of advanced biofuels; however, product yields remain well below those necessary for large scale production. New genetic tools and high throughput metabolic engineering techniques are needed to optimize cyanobacterial metabolisms for enhanced biofuel production. Towards this goal, this project advances the development of a multiple promoter replacement technique for systems-level optimization of gene expression in a model cyanobacterial host: Synechococcus sp. PCC 7002. To realize this multiple-target approach, key capabilities were developed, including a high throughput detection method for advanced biofuels, enhanced transformation efficiency, and genetic tools for Synechococcus sp. PCC 7002. Moreover, several additional obstacles were identified for realization of this multiple promoter replacement technique. The techniques and tools developed in this project will help to enable future efforts in the advancement of cyanobacterial biofuels.

• Research Organization: Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States)
• Sponsoring Organization: USDOE National Nuclear Security Administration (NNSA)
• DOE Contract Number: AC04-94AL85000
• OSTI ID: 1177597
• Report Number(s): SAND--2015-2319; 579481
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES