Application of an Efficient Gene Targeting System Linking Secondary Metabolites to their Biosynthetic Genes in Aspergillus terreus
Journal Article
·
· Organic Letters, 15(14):3562-3565
Nonribosomal peptides (NRPs) are natural products biosynthesized by NRP synthetases. A kusA-, pyrG- mutant strain of Aspergillusterreus NIH 2624 was developed that greatly facilitated the gene targeting efficiency in this organism. Application of this tool allowed us to link four major types of NRP related secondary metabolites to their responsible genes in A. terreus. In addition, an NRP related melanin synthetase was also identified in this species.
- Research Organization:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (US)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 1177332
- Report Number(s):
- PNNL-SA-95505; BM0101020
- Journal Information:
- Organic Letters, 15(14):3562-3565, Journal Name: Organic Letters, 15(14):3562-3565
- Country of Publication:
- United States
- Language:
- English
Similar Records
Molecular genetic analysis reveals that a nonribosomal peptide synthetase-like (NRPS-like) gene in Aspergillus nidulans is responsible for microperfuranone biosynthesis
Biosynthetic Pathway for the Epipolythiodioxopiperazine Acetylaranotin in Aspergillus terreus Revealed by Genome-based Deletion Analysis
Novel soil bacteria possess diverse genes for secondary metabolite biosynthesis
Journal Article
·
Tue Apr 10 00:00:00 EDT 2012
· Applied Microbiology and Biotechnology, 96(3):739-748
·
OSTI ID:1054474
Biosynthetic Pathway for the Epipolythiodioxopiperazine Acetylaranotin in Aspergillus terreus Revealed by Genome-based Deletion Analysis
Journal Article
·
Mon Apr 15 00:00:00 EDT 2013
· Journal of the American Chemical Society, 135(19):7205-7213
·
OSTI ID:1087806
Novel soil bacteria possess diverse genes for secondary metabolite biosynthesis
Journal Article
·
Wed Jun 13 00:00:00 EDT 2018
· Nature (London)
·
OSTI ID:1477308