1H NMR metabolomics study of metastatic melanoma in C57BL/6J mouse spleen

Wang, Xuan; Hu, Mary Y.; Feng, Ju; Liu, Maili; Hu, Jian Z.

doi:10.1007/s11306-014-0652-z

¹H NMR metabolomics study of metastatic melanoma in C57BL/6J mouse spleen

Journal Article · Thu Apr 03 00:00:00 EDT 2014 · Metabolomics

DOI:https://doi.org/10.1007/s11306-014-0652-z· OSTI ID:1176848

Wang, Xuan ^[1]; Hu, Mary Y. ^[2]; Feng, Ju ^[2]; Liu, Maili ^[3]; Hu, Jian Z. ^[2]

Pacific Northwest National Lab. (PNNL), Richland, WA (United States); The Chinese Academy of Sciences, Wuhan (People's Republic of China)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
The Chinese Academy of Sciences, Wuhan (People's Republic of China)

Melanoma is a malignant tumor of melanocytes. Although extensive investigations have been done to study metabolic changes in primary melanoma in vivo and in vitro, little effort has been devoted to metabolic profiling of metastatic tumors in organs other than lymph nodes. As such, in this work, NMR-based metabolomics combined with multivariate data analysis is used to study metastatic B16-F10 melanoma in C57BL/6J mouse spleen. Principal Component Analysis (PCA), an unsupervised multivariate data analysis method, is used to detect possible outliers, while Orthogonal Projection to Latent Structure (OPLS), a supervised multivariate data analysis method, is employed to find important metabolites responsible for discriminating the control and the melanoma groups. Two different strategies, i.e., spectral binning and spectral deconvolution, are used to reduce the original spectral data before statistical analysis. Spectral deconvolution is found to be superior for identifying a set of discriminatory metabolites between the control and the melanoma groups, especially when the sample size is small. OPLS results show that the melanoma group can be well separated from its control group. It is found that taurine, glutamate, aspartate, O-Phosphoethanolamine, niacinamide ,ATP, lipids and glycerol derivatives are decreased statistically and significantly while alanine, malate, xanthine, histamine, dCTP, GTP, thymidine, 2'-Deoxyguanosine are statistically and significantly elevated. Furthermore, these significantly changed metabolites are associated with multiple biological pathways and may be potential biomarkers for metastatic melanoma in spleen.

View Accepted Manuscript (DOE)

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL)

Sponsoring Organization:: National Institutes of Health (NIH), National Institute of Environmental Health Sciences (NIEHS); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 1176848

Report Number(s):: PNNL-SA--101943; 47841; 400412000

Journal Information:: Metabolomics, Journal Name: Metabolomics Journal Issue: 6 Vol. 10; ISSN 1573-3882

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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Related Subjects

1H NMR metabolomics
59 BASIC BIOLOGICAL SCIENCES
B16-F10 melanoma
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MULTIVARIATE ANALYSIS
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SPLEEN
metabolomics
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spleen