Detection of protease and protease activity using a single nanoscrescent SERS probe

Liu, Gang L.; Ellman, Jonathan A.; Lee, Luke P.; Chen, Fanqing Frank

Title: Detection of protease and protease activity using a single nanoscrescent SERS probe

Patent · Tue Jan 29 00:00:00 EST 2013

OSTI ID:1176495

Liu, Gang L.; Ellman, Jonathan A.; Lee, Luke P.; Chen, Fanqing Frank

This invention pertains to the in vitro detection of proteases using a single peptide-conjugate nanocrescent surface enhanced Raman scattering (SERS) probes with at least nanomolar sensitivity. The probe enables detection of proteolytic activity in extremely small volume and at low concentration. In certain embodiments the probes comprise an indicator for the detection of an active protease, where the indicator comprises a nanocrescent attached to a peptide, where said peptide comprises a recognition site for the protease and a Raman tag attached to the peptide.

View Patent

Cite

Export

Save

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC02-05CH11231

Assignee:: The Regents of the University of California (Oakland, CA)

Patent Number(s):: 8,361,932

Application Number:: 12/299,284

OSTI ID:: 1176495

Resource Relation:: Patent File Date: 2007 May 02

Country of Publication:: United States

Language:: English

References (30)

Human Kallikrein 2 (hK2) and Prostate-Specific Antigen (PSA): Two Closely Related, but Distinct, Kallikreins in the Prostate Rittenhouse, Harry G.; Finlay, Judith A.; Mikolajczyk, Stephen D. Critical Reviews in Clinical Laboratory Sciences, Vol. 35, Issue 4 https://doi.org/10.1080/10408369891234219	journal	January 1998
Pro PSA: a more cancer specific form of prostate specific antigen for the early detection of prostate cancer. Mikolajczyk, Stephen D.; Rittenhouse, Harry G. The Keio Journal of Medicine, Vol. 52, Issue 2 https://doi.org/10.2302/kjm.52.86	journal	January 2003
Nanophotonic Crescent Moon Structures with Sharp Edge for Ultrasensitive Biomolecular Detection by Local Electromagnetic Field Enhancement Effect Lu, Yu; Liu, Gang L.; Kim, Jaeyoun Nano Letters, Vol. 5, Issue 1, p. 119-124 https://doi.org/10.1021/nl048232+	journal	December 2004
Surface-enhanced Raman scattering on tunable plasmonic nanoparticle substrates Jackson, J. B.; Halas, N. J. Proceedings of the National Academy of Sciences, Vol. 101, Issue 52, p. 17930-17935 https://doi.org/10.1073/pnas.0408319102	journal	December 2004
Probing Single Molecules and Single Nanoparticles by Surface-Enhanced Raman Scattering Nie, Shuming; Emory, Steven R. Science, Vol. 275, Issue 5303, p. 1102-1106 https://doi.org/10.1126/science.275.5303.1102	journal	February 1997
A Change of Wave-length in Light Scattering Raman, C. V. Nature, Vol. 121, Issue 3051 https://doi.org/10.1038/121619b0	journal	April 1928
Prostate cancer epidemiology Grönberg, Henrik The Lancet, Vol. 361, Issue 9360 https://doi.org/10.1016/S0140-6736(03)12713-4	journal	March 2003
Prostate-Specific Antigen-Activated Thapsigargin Prodrug as Targeted Therapy for Prostate Cancer Denmeade, S. R.; Jakobsen, C. M.; Janssen, S. JNCI Journal of the National Cancer Institute, Vol. 95, Issue 13 https://doi.org/10.1093/jnci/95.13.990	journal	July 2003
Detection of a Biomarker for Alzheimer's Disease from Synthetic and Clinical Samples Using a Nanoscale Optical Biosensor Haes, Amanda J.; Chang, Lei; Klein, William L. Journal of the American Chemical Society, Vol. 127, Issue 7 https://doi.org/10.1021/ja044087q	journal	February 2005
Design of new and sensitive fluorogenic substrates for human kallikrein hK3 (prostate-specific antigen) derived from semenogelin sequences Réhault, Sophie; Brillard-Bourdet, Michèle; Bourgeois, Luc Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol. 1596, Issue 1 https://doi.org/10.1016/S0167-4838(02)00204-2	journal	April 2002
Characterization of Prostate-Specific Antigen Proteolytic Activity on its Major Physiological Substrate, the Sperm Motility Inhibitor Precursor/Semenogelin I ^† Robert, Martin; Gibbs, Bernard F.; Jacobson, Elka Biochemistry, Vol. 36, Issue 13 https://doi.org/10.1021/bi9626158	journal	April 1997
Amidolytic activity of prostatic acid phosphatase on human semenogelins and semenogelin-derived synthetic substrates: Amidolytic activity of prostatic acid phosphatase Brillard-Bourdet, Michèle; Réhault, Sophie; Juliano, Luiz European Journal of Biochemistry, Vol. 269, Issue 1 https://doi.org/10.1046/j.0014-2956.2001.02667.x	journal	January 2002
Optofluidic control using photothermal nanoparticles Liu, Gang L.; Kim, Jaeyoun; Lu, Yu Nature Materials, Vol. 5, Issue 1 https://doi.org/10.1038/nmat1528	journal	December 2005
Two-dimensional electrophoresis of prostate-specific antigen in sera of men with prostate cancer or benign prostate hyperplasia Charrier, Jean-Philippe; Tournel, Carole; Michel, Sandrine Electrophoresis, Vol. 20, Issue 4-5, p. 1075-1081 https://doi.org/10.1002/(SICI)1522-2683(19990101)20:4/5<1075::AID-ELPS1075>3.0.CO;2-K	journal	January 1999
Epidemiology of prostate cancer: molecular and environmental clues Pienta, Kenneth J.; Goodson, Jo Ann; Esper, Peggy S. Urology, Vol. 48, Issue 5, p. 676-683 https://doi.org/10.1016/S0090-4295(96)00219-1	journal	November 1996
Separation of enzymatically active and inactive prostate-specific antigen (PSA) by peptide affinity chromatography: PSA-Specific Peptides Wu, Ping; Stenman, Ulf-Håkan; Pakkala, Miikka The Prostate, Vol. 58, Issue 4 https://doi.org/10.1002/pros.10337	journal	November 2003
Raman-based system for DNA sequencing-mapping and other separations Vo-Dinh, Tuan https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5306403 US Patent Document 5,306,403	patent	April 1994
Time-resolved fluorescence imaging (TRFI) for direct immunofluorescence of PSA and alpha-1-antichymotrypsin in prostatic tissue sections Bjartell, A.; Siivola, P.; Hulkko, S. Prostate Cancer and Prostatic Diseases, Vol. 2, Issue 3 https://doi.org/10.1038/sj.pcan.4500307	journal	May 1999
Epidemiology of prostate cancer Crawford, E. David Urology, Vol. 62, Issue 6, p. 3-12 https://doi.org/10.1016/j.urology.2003.10.013	journal	December 2003
Are multiple markers the future of prostate cancer diagnostics? Mikolajczyk, Stephen D.; Song, Yong; Wong, James R. Clinical Biochemistry, Vol. 37, Issue 7, p. 519-528 https://doi.org/10.1016/j.clinbiochem.2004.05.016	journal	July 2004
High-Density Silver Nanoparticle Film with Temperature-Controllable Interparticle Spacing for a Tunable Surface Enhanced Raman Scattering Substrate Lu, Yu; Liu, Gang L.; Lee, Luke P. Nano Letters, Vol. 5, Issue 1 https://doi.org/10.1021/nl048965u	journal	January 2005
Immunopeptidometric Assay for Enzymatically Active Prostate-Specific Antigen Wu, P. Clinical Chemistry, Vol. 50, Issue 1 https://doi.org/10.1373/clinchem.2003.026146	journal	January 2004
The New Human Tissue Kallikrein Gene Family: Structure, Function, and Association to Disease Yousef, G. M. Endocrine Reviews, Vol. 22, Issue 2 https://doi.org/10.1210/er.22.2.184	journal	April 2001
Development and validation of a quantitative ELISA for the measurement of PSA concentration Acevedo, Boris; Perera, Yasser; Ruiz, Miriam Clinica Chimica Acta, Vol. 317, Issue 1-2, p. 55-63 https://doi.org/10.1016/S0009-8981(01)00749-5	journal	March 2002
Peptide-Nanoparticle Hybrid SERS Probe for Dynamic Detection of Active Cancer Biomarker Enzymes Liu, Gang L.; Chen, F. Frank; Ellman, Jonathan A. Conference Proceedings. Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 2006 International Conference of the IEEE Engineering in Medicine and Biology Society https://doi.org/10.1109/IEMBS.2006.260259	conference	August 2006
Enzymatic action of prostate-specific antigen (PSA or hK3): Substrate specificity and regulation by Zn2+, a tight-binding inhibitor Malm, Johan; Hellman, Jukka; Hogg, Phil The Prostate, Vol. 45, Issue 2, p. 132-139 https://doi.org/10.1002/1097-0045(20001001)45:2<132::AID-PROS7>3.0.CO;2-3	journal	January 2000
Proenzyme Forms of Prostate-Specific Antigen in Serum Improve the Detection of Prostate Cancer Mikolajczyk, S. D. Clinical Chemistry, Vol. 50, Issue 6 https://doi.org/10.1373/clinchem.2003.026823	journal	June 2004
A history of prostate cancer treatment Denmeade, Samuel R.; Isaacs, John T. Nature Reviews Cancer, Vol. 2, Issue 5 https://doi.org/10.1038/nrc801	journal	May 2002
Magnetic Nanocrescents as Controllable Surface-Enhanced Raman Scattering Nanoprobes for Biomolecular Imaging Liu, G. L.; Lu, Y.; Kim, J. Advanced Materials, Vol. 17, Issue 22, p. 2683-2688 https://doi.org/10.1002/adma.200501064	journal	November 2005
Nanowell surface enhanced Raman scattering arrays fabricated by soft-lithography for label-free biomolecular detections in integrated microfluidics Liu, Gang L.; Lee, Luke P. Applied Physics Letters, Vol. 87, Issue 7 https://doi.org/10.1063/1.2031935	journal	August 2005

Similar Records

Detection of protease and protease activity using a single nanocrescent SERS probe

Patent · Tue Sep 29 00:00:00 EDT 2015 · OSTI ID:1176495

Liu, Gang L.; Ellman, Jonathan A.; Lee, Luke P.; +1 more

Crystal Structure of the VP4 Protease from Infectious Pancreatic Necrosis Virus Reveals the acyl-enzyme Complex for an Intermolecular Self-Cleavage Reaction

Journal Article · Mon Jan 01 00:00:00 EST 2007 · Journal of Biological Chemistry · OSTI ID:1176495

Lee, J; Feldman, A; Delmas, B; +1 more

Purification and characterization of two functional forms of intracellular protease PfpI from the hyperthermophilic archaeon Pyrococcus furiosus

Journal Article · Wed Jan 01 00:00:00 EST 1997 · Applied and Environmental Microbiology · OSTI ID:1176495

Halio, S B; Bauer, M W; Kelley, R M

Related Subjects

60 APPLIED LIFE SCIENCES
59 BASIC BIOLOGICAL SCIENCES
47 OTHER INSTRUMENTATION

Title: Detection of protease and protease activity using a single nanoscrescent SERS probe

Citation Formats

References (30)

Similar Records

Related Subjects