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Office of Scientific and Technical Information
 

Non-focusing optics spectrophotometer, and methods of use

Patent ·
OSTI ID:1175116
;
In one aspect, the present invention provides kinetic spectrophotometers that each comprise: (a) a light source; and (b) a compound parabolic concentrator disposed to receive light from the light source and configured to (1) intensify and diffuse the light received from the light source, and (2) direct the intensified and diffused light onto a sample. In other aspects, the present invention provides methods for measuring a photosynthetic parameter, the methods comprising the steps of: (a) illuminating a plant leaf until steady-state photosynthesis is achieved; (b) subjecting the illuminated plant leaf to a period of darkness; (c) using a kinetic spectrophotometer of the invention to collect spectral data from the plant leaf treated in accordance with steps (a) and (b); and (d) determining a value for a photosynthetic parameter from the spectral data.
Research Organization:
Washington State University Research Foundation, Pullman, WA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG03-98ER20299;
Assignee:
Washington State University Research Foundation
Patent Number(s):
6,813,024
Application Number:
09/785,041
OSTI ID:
1175116
Country of Publication:
United States
Language:
English

References (10)

Control and Measurement of Photosynthetic Electron Transport in Vivo book
H+/E- Ratios for Photosynthetic Electron Transfer in Intact Leaves in the Steady State. book January 1998
A portable multi-flash kinetic fluorimeter for measurement of donor and acceptor reactions of Photosystem 2 in leaves of intact plants under field conditions journal December 1990
The relationship between non-photochemical quenching of chlorophyll fluorescence and the rate of photosystem 2 photochemistry in leaves journal September 1990
Electron transfer between the two photosystems. I. Flash excitation under oxidizing conditions journal May 1984
Activation of the chloroplast ATPase measured by the electrochromic change in leaves of intact plants journal August 1989
A diffused-optics flash kinetic spectrophotometer (DOFS) for measurements of absorbance changes in intact plants in the steady-state journal April 1998
The proton to electron stoichiometry of steady-state photosynthesis in living plants: A proton-pumping Q cycle is continuously engaged journal December 2000
Dissipation of the Proton Electrochemical Potential in Intact and Lysed Chloroplasts: I. The Electrical Potential journal February 1991
Compound parabolic concentrator probe for efficient light collection in spectroscopy of biological tissue journal January 1996

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