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Title: Metal binding proteins, recombinant host cells and methods

Abstract

The present disclosure provides artificial heavy metal binding proteins termed chelons by the inventors. These chelons bind cadmium and/or mercuric ions with relatively high affinity. Also disclosed are coding sequences, recombinant DNA molecules and recombinant host cells comprising those recombinant DNA molecules for expression of the chelon proteins. In the recombinant host cells or transgenic plants, the chelons can be used to bind heavy metals taken up from contaminated soil, groundwater or irrigation water and to concentrate and sequester those ions. Recombinant enteric bacteria can be used within the gastrointestinal tracts of animals or humans exposed to toxic metal ions such as mercury and/or cadmium, where the chelon recombinantly expressed in chosen in accordance with the ion to be rededicated. Alternatively, the chelons can be immobilized to solid supports to bind and concentrate heavy metals from a contaminated aqueous medium including biological fluids.

Inventors:
;
Publication Date:
Research Org.:
University of Georgia Research Foundation, Inc., Athens, GA (Unites States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1174899
Patent Number(s):
6,750,042
Application Number:
09/977,137
Assignee:
University of Georgia Research Foundation, Inc. (Athens, GA) OSTI
DOE Contract Number:
FG02-99ER62865
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Summers, Anne O., and Caguiat, Jonathan J.. Metal binding proteins, recombinant host cells and methods. United States: N. p., 2004. Web.
Summers, Anne O., & Caguiat, Jonathan J.. Metal binding proteins, recombinant host cells and methods. United States.
Summers, Anne O., and Caguiat, Jonathan J.. Tue . "Metal binding proteins, recombinant host cells and methods". United States. doi:. https://www.osti.gov/servlets/purl/1174899.
@article{osti_1174899,
title = {Metal binding proteins, recombinant host cells and methods},
author = {Summers, Anne O. and Caguiat, Jonathan J.},
abstractNote = {The present disclosure provides artificial heavy metal binding proteins termed chelons by the inventors. These chelons bind cadmium and/or mercuric ions with relatively high affinity. Also disclosed are coding sequences, recombinant DNA molecules and recombinant host cells comprising those recombinant DNA molecules for expression of the chelon proteins. In the recombinant host cells or transgenic plants, the chelons can be used to bind heavy metals taken up from contaminated soil, groundwater or irrigation water and to concentrate and sequester those ions. Recombinant enteric bacteria can be used within the gastrointestinal tracts of animals or humans exposed to toxic metal ions such as mercury and/or cadmium, where the chelon recombinantly expressed in chosen in accordance with the ion to be rededicated. Alternatively, the chelons can be immobilized to solid supports to bind and concentrate heavy metals from a contaminated aqueous medium including biological fluids.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jun 15 00:00:00 EDT 2004},
month = {Tue Jun 15 00:00:00 EDT 2004}
}

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