Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation

Schaff, Ulrich Y; Koh, Chung-Yan; Sommer, Gregory J

Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation

Patent · 24 February 2015

OSTI ID:1171088

Schaff, Ulrich Y; Koh, Chung-Yan; Sommer, Gregory J

Embodiments of the present invention are directed toward devices, systems, and method for conducting assays using sedimentation. In one example, a method includes layering a mixture on a density medium, subjecting sedimentation particles in the mixture to sedimentation forces to cause the sedimentation particles to move to a detection area through a density medium, and detecting a target analyte in a detection region of the sedimentation channel. In some examples, the sedimentation particles and labeling agent may have like charges to reduce non-specific binding of labeling agent and sedimentation particles. In some examples, the density medium is provided with a separation layer for stabilizing the assay during storage and operation. In some examples, the sedimentation channel may be provided with a generally flat sedimentation chamber for dispersing the particle pellet over a larger surface area.

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Research Organization:: Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC04-94AL85000

Assignee:: Sandia Corporation (Albuquerque, NM)

Patent Number(s):: 8,962,346

Application Number:: 13/423,050

OSTI ID:: 1171088

Country of Publication:: United States

Language:: English

References (54)

Microfabrication of a tapered channel for isoelectric focusing with thermally generated pH gradient Huang, Tiemin; Pawliszyn, Janusz ELECTROPHORESIS, Vol. 23, Issue 20, p. 3504-3510 https://doi.org/10.1002/1522-2683(200210)23:20<3504::AID-ELPS3504>3.0.CO;2-X	journal	October 2002
Whole Blood Immunoassay Based on Centrifugal Bead Sedimentation Schaff, U. Y.; Sommer, G. J. Clinical Chemistry, Vol. 57, Issue 5, p. 753-761 https://doi.org/10.1373/clinchem.2011.162206	journal	March 2011
Plasma DNA concentration as a predictor of mortality and sepsis in critically ill patients Rhodes, Andrew; Wort, Stephen J.; Thomas, Helen Critical Care, Vol. 10, Issue 2, Article No. R60 https://doi.org/10.1186/cc4894	journal	April 2006
Shiga toxin 1 acting on DNA in vitro is a heat-stable enzyme not requiring proteolytic activation Brigotti, Maurizio; Carnicelli, Domenica; Vara, Antonio González Biochimie, Vol. 86, Issue 4-5, p. 305-309 https://doi.org/10.1016/j.biochi.2004.03.009	journal	April 2004
Infrared controlled waxes for liquid handling and storage on a CD-microfluidic platform Abi-Samra, Kameel; Hanson, Ryan; Madou, Marc Lab on a Chip, Vol. 11, Issue 4, p. 723-726 https://doi.org/10.1039/C0LC00160K	journal	January 2011
A Method for Global Analysis of Complex Proteomes Using Sample Prefractionation by Solution Isoelectrofocusing Prior to Two-Dimensional Electrophoresis Zuo, Xun; Speicher, David W. Analytical Biochemistry, Vol. 284, Issue 2 https://doi.org/10.1006/abio.2000.4714	journal	September 2000
A rapid direct fluorescent assay for cell-free DNA quantification in biological fluids Goldshtein, H.; Hausmann, M. J; Douvdevani, A. Annals of Clinical Biochemistry, Vol. 46, Issue 6, p. 488-494 https://doi.org/10.1258/acb.2009.009002	journal	September 2009
The Alpher, Bethe, Gamow of isoelectric focusing, the alpha-Centaury of electrokinetic methodologies. Part I Righetti, Pier Giorgio ELECTROPHORESIS, Vol. 27, Issue 5-6, p. 923-938 https://doi.org/10.1002/elps.200500525	journal	March 2006
Integration of nanoporous membranes for sample filtration/preconcentration in microchip electrophoresis Long, Zhicheng; Liu, Dayu; Ye, Nannan ELECTROPHORESIS, Vol. 27, Issue 24, p. 4927-4934 https://doi.org/10.1002/elps.200600252	journal	December 2006
Detecting Single Stranded DNA with a Solid State Nanopore Fologea, Daniel; Gershow, Marc; Ledden, Bradley Nano Letters, Vol. 5, Issue 10, p. 1905-1909 https://doi.org/10.1021/nl051199m	journal	October 2005
Light scattering immunoassay Price, Christopher; Newman, David Principles and Practice of Immunoassay https://doi.org/10.1007/978-1-349-11234-0_16	book	January 1991
Bioinformatic processing to identify single nucleotide polymorphism that potentially affect Ape1 function Yu, Eizadora T.; Hadi, Masood Z. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Vol. 722, Issue 2, p. 140-146 https://doi.org/10.1016/j.mrgentox.2010.06.015	journal	June 2011
Bead-based microfluidic immunoassays: The next generation Lim, C. T.; Zhang, Y. Biosensors and Bioelectronics, Vol. 22, Issue 7, p. 1197-1204 https://doi.org/10.1016/j.bios.2006.06.005	journal	February 2007
A molecular approach to bioseparations: Protein–protein and protein–salt interactions Curtis, R. A.; Lue, L. Chemical Engineering Science, Vol. 61, Issue 3, p. 907-923 https://doi.org/10.1016/j.ces.2005.04.007	journal	February 2006
How Hofmeister ion interactions affect protein stability Baldwin, R. L. Biophysical Journal, Vol. 71, Issue 4, p. 2056-2063 https://doi.org/10.1016/S0006-3495(96)79404-3	journal	October 1996
A B Cell-Based Sensor for Rapid Identification of Pathogens Rider, Todd H.; Petrovick, Martha S.; Nargi, Frances E. Science, Vol. 301, Issue 5630, p. 213-215 https://doi.org/10.1126/science.1084920	journal	July 2003
Fully integrated lab-on-a-disc for simultaneous analysis of biochemistry and immunoassay from whole blood Lee, Beom Seok; Lee, Yang Ui; Kim, Han-Sang Lab on a Chip, Vol. 11, Issue 1, p. 70-78 https://doi.org/10.1039/C0LC00205D	journal	January 2011
Parallel processing in the isoelectric focusing chip Zilberstein, Gleb V.; Baskin, Emmanuil M.; Bukshpan, Shmuel ELECTROPHORESIS, Vol. 24, Issue 21, p. 3735-3744 https://doi.org/10.1002/elps.200305663	journal	November 2003
Lab On A CD Madou, Marc; Zoval, Jim; Jia, Guangyao Annual Review of Biomedical Engineering, Vol. 8, Issue 1, p. 601-628 https://doi.org/10.1146/annurev.bioeng.8.061505.095758	journal	August 2006
Microfluidic immunoassays as rapid saliva-based clinical diagnostics Herr, A. E.; Hatch, A. V.; Throckmorton, D. J. Proceedings of the National Academy of Sciences, Vol. 104, Issue 13, p. 5268-5273 https://doi.org/10.1073/pnas.0607254104	journal	March 2007
One-step purification of nucleic acid for gene expression analysis via Immiscible Filtration Assisted by Surface Tension (IFAST) Berry, Scott M.; Alarid, Elaine T.; Beebe, David J. Lab on a Chip, Vol. 11, Issue 10, p. 1747-1753 https://doi.org/10.1039/C1LC00004G	journal	January 2011
Rapid isoelectric trapping in a micropreparative-scale multicompartment electrolyzer Lim, Peniel; North, Robert; Vigh, Gyula ELECTROPHORESIS, Vol. 28, Issue 12, p. 1851-1859 https://doi.org/10.1002/elps.200600846	journal	June 2007
Multistage Isoelectric Focusing in a Polymeric Microfluidic Chip Cui, Huanchun; Horiuchi, Keisuke; Dutta, Prashanta Analytical Chemistry, Vol. 77, Issue 24, p. 7878-7886 https://doi.org/10.1021/ac050781s	journal	December 2005
On-Chip Coupling of Isoelectric Focusing and Free Solution Electrophoresis for Multidimensional Separations Herr, Amy E.; Molho, Joshua I.; Drouvalakis, Katerina A. Analytical Chemistry, Vol. 75, Issue 5, p. 1180-1187 https://doi.org/10.1021/ac026239a	journal	March 2003
Cell-Free DNA—A Marker to Predict Ischemic Brain Damage in a Rat Stroke Experimental Model Boyko, Matthew; Ohayon, Sharon; Goldsmith, Tomer Journal of Neurosurgical Anesthesiology, Vol. 23, Issue 3, p. 222-228 https://doi.org/10.1097/ANA.0b013e31821b536a	journal	January 2011
The Alpher, Bethe and Gamow of IEF, the alpha-Centaury of electrokinetic methodologies. Part II: Immobilized pH gradients Righetti, Pier Giorgio ELECTROPHORESIS, Vol. 28, Issue 4, p. 545-555 https://doi.org/10.1002/elps.200600256	journal	February 2007
Ricin A-Chain Activity on Stem−Loop and Unstructured DNA Substrates Amukele, Tim K.; Roday, Setu; Schramm, Vern L. Biochemistry, Vol. 44, Issue 11, p. 4416-4425 https://doi.org/10.1021/bi0474362	journal	March 2005
On-Chip Isoelectric Focusing Using Photopolymerized Immobilized pH Gradients Sommer, Greg J.; Singh, Anup K.; Hatch, Anson V. Analytical Chemistry, Vol. 80, Issue 9, p. 3327-3333 https://doi.org/10.1021/ac702523g	journal	May 2008
Centrifugal microfluidics for biomedical applications Gorkin, Robert; Park, Jiwoon; Siegrist, Jonathan Lab on a Chip, Vol. 10, Issue 14, p. 1758-1773 https://doi.org/10.1039/B924109D	journal	January 2010
Parallel Nanoliter Microfluidic Analysis System Andersson, Per; Jesson, Gerald; Kylberg, Gunnar Analytical Chemistry, Vol. 79, Issue 11, p. 4022-4030 https://doi.org/10.1021/ac061692y	journal	June 2007
Staphylococcal Enterotoxin B as a Biological Weapon: Recognition, Management, and Surveillance of Staphylococcal Enterotoxin Ahanotu, Ejem; Alvelo-Ceron, Damaris; Ravita, Timothy Applied Biosafety, Vol. 11, Issue 3, p. 120-126 https://doi.org/10.1177/153567600601100303	journal	September 2006
Cell-Free Plasma DNA as a Predictor of Outcome in Severe Sepsis and Septic Shock Saukkonen, K.; Lakkisto, P.; Pettila, V. Clinical Chemistry, Vol. 54, Issue 6, p. 1000-1007 https://doi.org/10.1373/clinchem.2007.101030	journal	April 2008
Functional integration of DNA purification and concentration into a real time micro-PCR chip Min, Junhong; Kim, Joon-Ho; Lee, Youngsun Lab on a Chip, Vol. 11, Issue 2, p. 259-265 https://doi.org/10.1039/C0LC00320D	journal	January 2011
Measurement of Circulating Cell-Free DNA Levels by a New Simple Fluorescent Test in Patients With Primary Colorectal Cancer Czeiger, David; Shaked, Gad; Eini, Hadar American Journal of Clinical Pathology, Vol. 135, Issue 2, p. 264-270 https://doi.org/10.1309/AJCP4RK2IHVKTTZV	journal	February 2011
A new biodosimetric method: branched DNA-based quantitative detection of B1 DNA in mouse plasma Zhang, L.; Zhang, M.; Yang, S. The British Journal of Radiology, Vol. 83, Issue 992, p. 694-701 https://doi.org/10.1259/bjr/49886569	journal	August 2010
Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometry Holmes, David; Pettigrew, David; Reccius, Christian H. Lab on a Chip, Vol. 9, Issue 20, p. 2881-2889 https://doi.org/10.1039/B910053A	journal	January 2009
Circulating DNA: a new diagnostic gold mine? Ziegler, Annemarie; Zangemeister-Wittke, Uwe; Stahel, Rolf A. Cancer Treatment Reviews, Vol. 28, Issue 5, p. 255-271 https://doi.org/10.1016/S0305-7372(02)00077-4	journal	October 2002
Formation of Natural pH Gradients in a Microfluidic Device under Flow Conditions: Model and Experimental Validation Cabrera, Catherine R.; Finlayson, Bruce; Yager, Paul Analytical Chemistry, Vol. 73, Issue 3, p. 658-666 https://doi.org/10.1021/ac000495a	journal	February 2001
Miniaturized capillary isoelectric focusing in plastic microfluidic devices Tan, Woei; Fan, Z. Hugh; Qiu, Charmaine X. ELECTROPHORESIS, Vol. 23, Issue 20, p. 3638-3645 https://doi.org/10.1002/1522-2683(200210)23:20<3638::AID-ELPS3638>3.0.CO;2-Y	journal	October 2002
Depurination of A4256 in 28 S rRNA by the Ribosome-inactivating Proteins from Barley and Ricin Results in Different Ribosome Conformations Holmberg, Lovisa; Nygård, Odd Journal of Molecular Biology, Vol. 259, Issue 1, p. 81-94 https://doi.org/10.1006/jmbi.1996.0303	journal	May 1996
Effects of separation length and voltage on isoelectric focusing in a plastic microfluidic device Das, Champak; Fan, Z. Hugh ELECTROPHORESIS, Vol. 27, Issue 18, p. 3619-3626 https://doi.org/10.1002/elps.200600013	journal	September 2006
Integrated Preconcentration SDS−PAGE of Proteins in Microchips Using Photopatterned Cross-Linked Polyacrylamide Gels Hatch, Anson V.; Herr, Amy E.; Throckmorton, Daniel J. Analytical Chemistry, Vol. 78, Issue 14, p. 4976-4984 https://doi.org/10.1021/ac0600454	journal	July 2006
Characterization of EBV-genome negative “null” and “T” cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma Schneider, Ulrich; Schwenk, Hans-Ulrich; Bornkamm, Georg International Journal of Cancer, Vol. 19, Issue 5, p. 621-626 https://doi.org/10.1002/ijc.2910190505	journal	May 1977
Read-out concepts for multiplexed bead-based fluorescence immunoassays on centrifugal microfluidic platforms Riegger, L.; Grumann, M.; Nann, T. Sensors and Actuators A: Physical, Vol. 126, Issue 2, p. 455-462 https://doi.org/10.1016/j.sna.2005.11.006	journal	February 2006
Preparative-scale isoelectric trapping separations using a modified Gradiflow unit Ogle, David; Ho, Agnes; Gibson, Toby Journal of Chromatography A, Vol. 979, Issue 1-2, p. 155-161 https://doi.org/10.1016/S0021-9673(02)01601-1	journal	December 2002
Recent developments in two-dimensional gel electrophoresis with immobilized pH gradients: Wide pH gradients up to pH 12, longer separation distances and simplified procedures Görg, Angelika; Obermaier, Christian; Boguth, Günther Electrophoresis, Vol. 20, Issue 4-5, p. 712-717 https://doi.org/10.1002/(SICI)1522-2683(19990101)20:4/5<712::AID-ELPS712>3.0.CO;2-Y	journal	January 1999
Parallel isoelectric focusing II Zilberstein, Gleb V.; Baskin, Emmanuil M.; Bukshpan, Shmuel ELECTROPHORESIS, Vol. 25, Issue 21-22, p. 3643-3651 https://doi.org/10.1002/elps.200406117	journal	November 2004
Parallel isoelectric focusing chip Zilberstein, Gleb; Korol, Leonid; Bukshpan, Shmuel PROTEOMICS, Vol. 4, Issue 9, p. 2533-2540 https://doi.org/10.1002/pmic.200300794	journal	September 2004
Design Optimization of Spirally Grooved Thrust Air Bearings for Polygon Mirror Laser Scanners Satomi, Tadaatsu; Lin, Gang JSME international journal. Ser. C, Dynamics, control, robotics, design and manufacturing, Vol. 36, Issue 3 https://doi.org/10.1299/jsmec1993.36.393	journal	January 1993
Comparison of sample fixation and the use of LDS-751 or anti-CD45 for leukocyte identification in mouse whole blood for flow cytometry Maes, Melissa L.; Davidson, Lisa B.; McDonagh, Paul F. Journal of Immunological Methods, Vol. 319, Issue 1-2, p. 79-86 https://doi.org/10.1016/j.jim.2006.10.016	journal	January 2007
The current state of two-dimensional electrophoresis with immobilized pH gradients Görg, Angelika; Obermaier, Christian; Boguth, Günther Electrophoresis, Vol. 21, Issue 6, p. 1037-1053 https://doi.org/10.1002/(SICI)1522-2683(20000401)21:6<1037::AID-ELPS1037>3.0.CO;2-V	journal	April 2000
A fully automated immunoassay from whole blood on a disc Lee, Beom Seok; Lee, Jung-Nam; Park, Jong-Myeon Lab on a Chip, Vol. 9, Issue 11, p. 1548-1555 https://doi.org/10.1039/B820321K	journal	January 2009
Photopolymerized diffusion-defined polyacrylamide gradient gels for on-chip protein sizing Lo, Catherine T.; Throckmorton, Daniel J.; Singh, Anup K. Lab on a Chip, Vol. 8, Issue 8, p. 1273-1279 https://doi.org/10.1039/B804485F	journal	January 2008
Micro freef-low IEF enhanced by active cooling and functionalized gels Albrecht, Jacob W.; Jensen, Klavs F. ELECTROPHORESIS, Vol. 27, Issue 24, p. 4960-4969 https://doi.org/10.1002/elps.200600436	journal	December 2006

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