Crystal structures of translocator protein (TSPO) and mutant mimic of a human polymorphism

Li, Fei; Liu, Jian; Zheng, Yi; Garavito, R. Michael; Ferguson-Miller, Shelagh

doi:10.1126/science.1260590

Title: Crystal structures of translocator protein (TSPO) and mutant mimic of a human polymorphism

Journal Article · Fri Jul 17 00:00:00 EDT 2015 · Science

DOI:https://doi.org/10.1126/science.1260590· OSTI ID:1169354

Li, Fei; Liu, Jian; Zheng, Yi; Garavito, R. Michael; Ferguson-Miller, Shelagh ^[1]

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: UNIVERSITYNIH

OSTI ID:: 1169354

Journal Information:: Science, Vol. 347, Issue 01, 2015

Country of Publication:: United States

Language:: ENGLISH

Citation Metrics:

Cited by: 120 works

Citation information provided by
Web of Science

References (38)

Structure of the Mitochondrial Translocator Protein in Complex with a Diagnostic Ligand Jaremko, ukasz; Jaremko, M.; Giller, K. Science, Vol. 343, Issue 6177 https://doi.org/10.1126/science.1248725	journal	March 2014
Identification of conserved lipid/detergent-binding sites in a high-resolution structure of the membrane protein cytochrome c oxidase Qin, L.; Hiser, C.; Mulichak, A. Proceedings of the National Academy of Sciences, Vol. 103, Issue 44 https://doi.org/10.1073/pnas.0606149103	journal	October 2006
In Vivo and in Vitro Peripheral-Type Benzodiazepine Receptor Polymerization: Functional Significance in Drug Ligand and Cholesterol Binding ^† Delavoie, Franck; Li, Hua; Hardwick, Matthew Biochemistry, Vol. 42, Issue 15 https://doi.org/10.1021/bi0267487	journal	April 2003
An 18-kDa Translocator Protein (TSPO) Polymorphism Explains Differences in Binding Affinity of the PET Radioligand PBR28 Owen, David R.; Yeo, Astrid J.; Gunn, Roger N. Journal of Cerebral Blood Flow & Metabolism, Vol. 32, Issue 1 https://doi.org/10.1038/jcbfm.2011.147	journal	October 2011
Translocation Mechanism of Long Sugar Chains across the Maltoporin Membrane Channel Dutzler, Raimund; Schirmer, Tilman; Karplus, Martin Structure, Vol. 10, Issue 9 https://doi.org/10.1016/S0969-2126(02)00811-0	journal	September 2002
Ala147Thr substitution in translocator protein is associated with adult separation anxiety in patients with depression Costa, Barbara; Pini, Stefano; Martini, Claudia Psychiatric Genetics, Vol. 19, Issue 2 https://doi.org/10.1097/YPG.0b013e32832080f6	journal	January 2009
A Novel Mechanism for the Regulation of Photosynthesis Gene Expression by the TspO Outer Membrane Protein of Rhodobacter sphaeroides 2.4.1 Yeliseev, Alexei A.; Kaplan, Samuel Journal of Biological Chemistry, Vol. 274, Issue 30 https://doi.org/10.1074/jbc.274.30.21234	journal	July 1999
Protein-Protein Interactions in the Membrane: Sequence, Structural, and Biological Motifs Moore, David T.; Berger, Bryan W.; DeGrado, William F. Structure, Vol. 16, Issue 7 https://doi.org/10.1016/j.str.2008.05.007	journal	July 2008
Characterization and Modeling of the Oligomeric State and Ligand Binding Behavior of Purified Translocator Protein 18 kDa from Rhodobacter sphaeroides Li, Fei; Xia, Yan; Meiler, Jens Biochemistry, Vol. 52, Issue 34 https://doi.org/10.1021/bi400431t	journal	August 2013
Peripheral-type benzodiazepine receptor: structure and function of a cholesterol-binding protein in steroid and bile acid biosynthesis Lacapère, Jean-Jacques; Papadopoulos, Vassilios Steroids, Vol. 68, Issue 7-8 https://doi.org/10.1016/S0039-128X(03)00101-6	journal	September 2003
Secondary and tertiary structures of the transmembrane domains of the translocator protein TSPO determined by NMR. Stabilization of the TSPO tertiary fold upon ligand binding Murail, Samuel; Robert, Jean-Claude; Coïc, Yves-Marie Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1778, Issue 6 https://doi.org/10.1016/j.bbamem.2008.03.012	journal	June 2008
Evolutionary Origin of the Mitochondrial Cholesterol Transport Machinery Reveals a Universal Mechanism of Steroid Hormone Biosynthesis in Animals Fan, Jinjiang; Papadopoulos, Vassilios PLoS ONE, Vol. 8, Issue 10 https://doi.org/10.1371/journal.pone.0076701	journal	October 2013
Translocator protein (18 kDa) (TSPO) as a therapeutic target for neurological and psychiatric disorders Rupprecht, Rainer; Papadopoulos, Vassilios; Rammes, Gerhard Nature Reviews Drug Discovery, Vol. 9, Issue 12 https://doi.org/10.1038/nrd3295	journal	December 2010
Bipolar Disorder is associated with the rs6971 polymorphism in the gene encoding 18kDa Translocator Protein (TSPO) Colasanti, Alessandro; Owen, David R.; Grozeva, Detelina Psychoneuroendocrinology, Vol. 38, Issue 11 https://doi.org/10.1016/j.psyneuen.2013.07.007	journal	November 2013
UCSF Chimera?A visualization system for exploratory research and analysis Pettersen, Eric F.; Goddard, Thomas D.; Huang, Conrad C. Journal of Computational Chemistry, Vol. 25, Issue 13 https://doi.org/10.1002/jcc.20084	journal	January 2004
Topological Analysis of the Peripheral Benzodiazepine Receptor in Yeast Mitochondrial Membranes Supports a Five-transmembrane Structure Joseph-Liauzun, Evelyne; Delmas, Pascal; Shire, David Journal of Biological Chemistry, Vol. 273, Issue 4 https://doi.org/10.1074/jbc.273.4.2146	journal	January 1998
Imaging of Peripheral Benzodiazepine Receptor Expression as Biomarkers of Detrimental versus Beneficial Glial Responses in Mouse Models of Alzheimer's and Other CNS Pathologies Ji, B.; Maeda, J.; Sawada, M. Journal of Neuroscience, Vol. 28, Issue 47 https://doi.org/10.1523/JNEUROSCI.2312-08.2008	journal	November 2008
Structural and Functional Study of Reconstituted Peripheral Benzodiazepine Receptor Lacapère, Jean-Jacques; Delavoie, Franck; Li, Hua Biochemical and Biophysical Research Communications, Vol. 284, Issue 2 https://doi.org/10.1006/bbrc.2001.4975	journal	June 2001
PARP1 exhibits enhanced association and catalytic efficiency with γH2A.X-nucleosome Sharma, Deepti; De Falco, Louis; Padavattan, Sivaraman Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-019-13641-0	journal	December 2019
A Genetic Polymorphism for Translocator Protein 18 Kda Affects both in Vitro and in Vivo Radioligand Binding in Human Brain to this Putative Biomarker of Neuroinflammation Kreisl, William C.; Jenko, Kimberly J.; Hines, Christina S. Journal of Cerebral Blood Flow & Metabolism, Vol. 33, Issue 1 https://doi.org/10.1038/jcbfm.2012.131	journal	September 2012
ALINE : a WYSIWYG protein-sequence alignment editor for publication-quality alignments Bond, Charles Simon; Schüttelkopf, Alexander Wolfgang Acta Crystallographica Section D Biological Crystallography, Vol. 65, Issue 5 https://doi.org/10.1107/S0907444909007835	journal	April 2009
Cavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation Tillu, Vikas A.; Rae, James; Gao, Ya Nature Communications, Vol. 12, Issue 1 https://doi.org/10.1038/s41467-021-21035-4	journal	February 2021
The Spontaneous Ala147Thr Amino Acid Substitution within the Translocator Protein Influences Pregnenolone Production in Lymphomonocytes of Healthy Individuals Costa, Barbara; Pini, Stefano; Gabelloni, Pamela Endocrinology, Vol. 150, Issue 12 https://doi.org/10.1210/en.2009-0752	journal	October 2009
A mammalian mitochondrial drug receptor functions as a bacterial "oxygen" sensor Yeliseev, A. A.; Krueger, K. E.; Kaplan, S. Proceedings of the National Academy of Sciences, Vol. 94, Issue 10 https://doi.org/10.1073/pnas.94.10.5101	journal	May 1997
Chemical Catalysis by the Translocator Protein (18 kDa) Ginter, Christopher; Kiburu, Irene; Boudker, Olga Biochemistry, Vol. 52, Issue 21 https://doi.org/10.1021/bi400364z	journal	May 2013
Flexible structure alignment by chaining aligned fragment pairs allowing twists Ye, Y.; Godzik, A. Bioinformatics, Vol. 19, Issue Suppl 2 https://doi.org/10.1093/bioinformatics/btg1086	journal	September 2003
PHENIX: a comprehensive Python-based system for macromolecular structure solution Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221 https://doi.org/10.1107/S0907444909052925	journal	January 2010
Evaluation of translocator protein quantification as a tool for characterising macrophage burden in human carotid atherosclerosis Bird, J. L. E.; Izquierdo-Garcia, D.; Davies, J. R. Atherosclerosis, Vol. 210, Issue 2 https://doi.org/10.1016/j.atherosclerosis.2009.11.047	journal	June 2010
TOP : a new method for protein structure comparisons and similarity searches Lu, Guoguang Journal of Applied Crystallography, Vol. 33, Issue 1 https://doi.org/10.1107/S0021889899012339	journal	February 2000
TspO of Rhodobacter sphaeroides: A STRUCTURAL AND FUNCTIONAL MODEL FOR THE MAMMALIAN PERIPHERAL BENZODIAZEPINE RECEPTOR Yeliseev, Alexei A.; Kaplan, Samuel Journal of Biological Chemistry, Vol. 275, Issue 8 https://doi.org/10.1074/jbc.275.8.5657	journal	February 2000
Peripheral-Type Benzodiazepine Receptor Function in Cholesterol Transport. Identification of a Putative Cholesterol Recognition/Interaction Amino Acid Sequence and Consensus Pattern Li, H. Endocrinology, Vol. 139, Issue 12 https://doi.org/10.1210/en.139.12.4991	journal	December 1998
MUSTANG: A multiple structural alignment algorithm Konagurthu, Arun S.; Whisstock, James C.; Stuckey, Peter J. Proteins: Structure, Function, and Bioinformatics, Vol. 64, Issue 3 https://doi.org/10.1002/prot.20921	journal	May 2006
Translocator Protein/Peripheral Benzodiazepine Receptor Is Not Required for Steroid Hormone Biosynthesis Morohaku, Kanako; Pelton, Susanne H.; Daugherty, Daniel J. Endocrinology, Vol. 155, Issue 1 https://doi.org/10.1210/en.2013-1556	journal	January 2014
The origin of mitochondria in light of a fluid prokaryotic chromosome model Esser, Christian; Martin, William; Dagan, Tal Biology Letters, Vol. 3, Issue 2 https://doi.org/10.1098/rsbl.2006.0582	journal	December 2006
Three-Dimensional Structure of TspO by Electron Cryomicroscopy of Helical Crystals Korkhov, Vladimir M.; Sachse, Carsten; Short, Judith M. Structure, Vol. 18, Issue 6 https://doi.org/10.1016/j.str.2010.03.001	journal	June 2010
Evidence that variation in the peripheral benzodiazepine receptor(PBR) gene influences susceptibility to panic disorder Nakamura, Kazuhiko; Yamada, Kazuo; Iwayama, Yoshimi American Journal of Medical Genetics Part B: Neuropsychiatric Genetics, Vol. 141B, Issue 3 https://doi.org/10.1002/ajmg.b.30211	journal	January 2006
Peripheral-Type Benzodiazepine Receptor Function in Cholesterol Transport. Identification of a Putative Cholesterol Recognition/Interaction Amino Acid Sequence and Consensus Pattern ¹ Li, Hua; Papadopoulos, Vassilios Endocrinology, Vol. 139, Issue 12 https://doi.org/10.1210/endo.139.12.6390	journal	December 1998
PHENIX: a comprehensive Python-based system for macromolecular structure solution. Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Apollo - University of Cambridge Repository https://doi.org/10.17863/cam.45787	text	January 2010

Cited By (37)

The Mitochondrial Translocator Protein and the Emerging Link Between Oxidative Stress and Arrhythmias in the Diabetic Heart Ilkan, Zeki; Akar, Fadi G. Frontiers in Physiology, Vol. 9 https://doi.org/10.3389/fphys.2018.01518	journal	October 2018
PET Evaluation of Microglial Activation in Non-neurodegenerative Brain Diseases Ghadery, Christine; Best, Laura A.; Pavese, Nicola Current Neurology and Neuroscience Reports, Vol. 19, Issue 7 https://doi.org/10.1007/s11910-019-0951-x	journal	May 2019
Modified benzoxazolone derivative as 18-kDa TSPO ligand Kumari, Neelam; Chadha, Nidhi; Srivastava, Pooja Chemical Biology & Drug Design, Vol. 90, Issue 4 https://doi.org/10.1111/cbdd.12971	journal	April 2017
CRISPR/Cas9‒Mediated Tspo Gene Mutations Lead to Reduced Mitochondrial Membrane Potential and Steroid Formation in MA-10 Mouse Tumor Leydig Cells Fan, Jinjiang; Wang, Kevin; Zirkin, Barry Endocrinology, Vol. 159, Issue 2 https://doi.org/10.1210/en.2017-03065	journal	December 2017
Structural Prediction of the Dimeric Form of the Mammalian Translocator Membrane Protein TSPO: A Key Target for Brain Diagnostics Zeng, Juan; Guareschi, Riccardo; Damre, Mangesh International Journal of Molecular Sciences, Vol. 19, Issue 9 https://doi.org/10.3390/ijms19092588	journal	August 2018
Identification of new channels by systematic analysis of the mitochondrial outer membrane Krüger, Vivien; Becker, Thomas; Becker, Lars Journal of Cell Biology, Vol. 216, Issue 11 https://doi.org/10.1083/jcb.201706043	journal	September 2017
Cholesterol-mediated allosteric regulation of the mitochondrial translocator protein structure Jaipuria, Garima; Leonov, Andrei; Giller, Karin Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/ncomms14893	journal	March 2017
Determination and reduction of translocator protein (TSPO) ligand rs6971 discrimination Sokias, Renee; Werry, Eryn L.; Chua, Sook W. MedChemComm, Vol. 8, Issue 1 https://doi.org/10.1039/c6md00523c	journal	January 2017
Cholesterol accumulation, lipid droplet formation, and steroid production in Leydig cells: Role of translocator protein (18‐kDa) Chung, Jin‐Yong; Chen, Haolin; Papadopoulos, Vassilios Andrology, Vol. 8, Issue 3 https://doi.org/10.1111/andr.12733	journal	December 2019
Recent Developments in TSPO PET Imaging as A Biomarker of Neuroinflammation in Neurodegenerative Disorders Werry, Eryn L.; Bright, Fiona M.; Piguet, Olivier International Journal of Molecular Sciences, Vol. 20, Issue 13 https://doi.org/10.3390/ijms20133161	journal	June 2019
Perspectives in TSPO PET Imaging for Neurologic Diseases Song, Yoo Sung Nuclear Medicine and Molecular Imaging, Vol. 53, Issue 6 https://doi.org/10.1007/s13139-019-00620-y	journal	November 2019
Structure of the mammalian TSPO/PBR protein Jaremko, Mariusz; Jaremko, Łukasz; Jaipuria, Garima Biochemical Society Transactions, Vol. 43, Issue 4 https://doi.org/10.1042/bst20150029	journal	August 2015
Structure and activity of tryptophan-rich TSPO proteins Guo, Y.; Kalathur, R. C.; Liu, Q. Science, Vol. 347, Issue 6221 https://doi.org/10.1126/science.aaa1534	journal	January 2015
Effects of genetic variants in the TSPO gene on protein structure and stability Milenkovic, Vladimir M.; Bader, Stefanie; Sudria-Lopez, Daniel Universität Regensburg https://doi.org/10.5283/epub.37328	text	January 2018
Mitochondrial Outer Membrane Channels: Emerging Diversity in Transport Processes Becker, Thomas; Wagner, Richard BioEssays, Vol. 40, Issue 7 https://doi.org/10.1002/bies.201800013	journal	April 2018
PET imaging of [11C]PBR28 in Parkinson’s disease patients does not indicate increased binding to TSPO despite reduced dopamine transporter binding Varnäs, Katarina; Cselényi, Zsolt; Jucaite, Aurelija European Journal of Nuclear Medicine and Molecular Imaging, Vol. 46, Issue 2 https://doi.org/10.1007/s00259-018-4161-6	journal	October 2018
TSPO regulation in reactive gliotic diseases McNeela, Adam M.; Bernick, Charles; Hines, Rochelle M. Journal of Neuroscience Research, Vol. 96, Issue 6 https://doi.org/10.1002/jnr.24212	journal	January 2018
A unified structural model of the mammalian translocator protein (TSPO) Xia, Yan; Ledwitch, Kaitlyn; Kuenze, Georg Journal of Biomolecular NMR, Vol. 73, Issue 6-7 https://doi.org/10.1007/s10858-019-00257-1	journal	June 2019
Effects of genetic variants in the TSPO gene on protein structure and stability Milenkovic, Vladimir M.; Bader, Stefanie; Sudria-Lopez, Daniel PLOS ONE, Vol. 13, Issue 4 https://doi.org/10.1371/journal.pone.0195627	journal	April 2018
Genetic obesity increases pancreatic expression of mitochondrial proteins which regulate cholesterol efflux in BRIN-BD11 insulinoma cells Caridis, Anna-Maria; Lightbody, Richard J.; Tarlton, Jamie M. R. Bioscience Reports, Vol. 39, Issue 3 https://doi.org/10.1042/bsr20181155	journal	March 2019
Leydig cells: formation, function, and regulation† Zirkin, Barry R.; Papadopoulos, Vassilios Biology of Reproduction, Vol. 99, Issue 1 https://doi.org/10.1093/biolre/ioy059	journal	March 2018
TSPO2 translocates 5‐aminolevulinic acid into human erythroleukemia cells Manceau, Hana; Lefevre, Sophie D.; Mirmiran, Arienne Biology of the Cell, Vol. 112, Issue 4 https://doi.org/10.1111/boc.201900098	journal	April 2020
Overview of the Molecular Steps in Steroidogenesis of the GABAergic Neurosteroids Allopregnanolone and Pregnanolone Liang, Jennifer J.; Rasmusson, Ann M. Chronic Stress, Vol. 2 https://doi.org/10.1177/2470547018818555	journal	January 2018
Be Cautious with Crystal Structures of Membrane Proteins or Complexes Prepared in Detergents Guo, Youzhong Crystals, Vol. 10, Issue 2 https://doi.org/10.3390/cryst10020086	journal	February 2020
Steroidogenic abnormalities in translocator protein knockout mice and significance in the aging male Barron, Anna M.; Ji, Bin; Kito, Seiji Biochemical Journal, Vol. 475, Issue 1 https://doi.org/10.1042/bcj20170645	journal	January 2018
TSPO mutations in rats and a human polymorphism impair the rate of steroid synthesis Owen, David R.; Fan, Jinjiang; Campioli, Enrico Biochemical Journal, Vol. 474, Issue 23 https://doi.org/10.1042/bcj20170648	journal	November 2017
Structural Integrity of the A147T Polymorph of Mammalian TSPO Jaremko, Mariusz; Jaremko, Łukasz; Giller, Karin ChemBioChem, Vol. 16, Issue 10 https://doi.org/10.1002/cbic.201500217	journal	June 2015
Sifting through the surfeit of neuroinflammation tracers Cumming, Paul; Burgher, Bjorn; Patkar, Omkar Journal of Cerebral Blood Flow & Metabolism, Vol. 38, Issue 2 https://doi.org/10.1177/0271678x17748786	journal	December 2017
Characterization of the High-Affinity Drug Ligand Binding Site of Mouse Recombinant TSPO Iatmanen-Harbi, Soria; Senicourt, lucile; Papadopoulos, Vassilios International Journal of Molecular Sciences, Vol. 20, Issue 6 https://doi.org/10.3390/ijms20061444	journal	March 2019
Translocator protein (18 kDa): an update on its function in steroidogenesis Papadopoulos, V.; Fan, J.; Zirkin, B. Journal of Neuroendocrinology, Vol. 30, Issue 2 https://doi.org/10.1111/jne.12500	journal	February 2018
Structural Prediction of the Dimeric Form of the Mammalian Translocator Membrane Protein TSPO: A Key Target for Brain Diagnostics Zeng, Juan; Guareschi, Riccardo; Damre, Mangesh RWTH Aachen University https://doi.org/10.18154/rwth-conv-236158	text	January 2018
A Plant-Specific N-terminal Extension Reveals Evolutionary Functional Divergence within Translocator Proteins Jurkiewicz, Pawel; Senicourt, Lucile; Ayeb, Haitham iScience, Vol. 23, Issue 3 https://doi.org/10.1016/j.isci.2020.100889	journal	March 2020
TSPO ligands stimulate ZnPPIX transport and ROS accumulation leading to the inhibition of P. falciparum growth in human blood Marginedas-Freixa, I.; Hattab, C.; Bouyer, G. Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep33516	journal	September 2016
Amhr2-Cre–Mediated Global Tspo Knockout Fan, Jinjiang; Campioli, Enrico; Sottas, Chantal Journal of the Endocrine Society, Vol. 4, Issue 2 https://doi.org/10.1210/jendso/bvaa001	journal	January 2020
Global Deletion of TSPO Does Not Affect the Viability and Gene Expression Profile Wang, Huaishan; Zhai, Kangle; Xue, Yingchao PLOS ONE, Vol. 11, Issue 12 https://doi.org/10.1371/journal.pone.0167307	journal	December 2016
Adrenal Mitochondria and Steroidogenesis: From Individual Proteins to Functional Protein Assemblies Midzak, Andrew; Papadopoulos, Vassilios Frontiers in Endocrinology, Vol. 7 https://doi.org/10.3389/fendo.2016.00106	journal	July 2016
Tetrapyrroles as Endogenous TSPO Ligands in Eukaryotes and Prokaryotes: Comparisons with Synthetic Ligands Veenman, Leo; Vainshtein, Alex; Yasin, Nasra International Journal of Molecular Sciences, Vol. 17, Issue 6 https://doi.org/10.3390/ijms17060880	journal	June 2016

Similar Records

Response to Comment on "Crystal structures of translocator protein (TSPO) and mutant mimic of a human polymorphism"

Journal Article · Thu Oct 29 00:00:00 EDT 2015 · Science · OSTI ID:1169354

Li, Fei; Liu, Jian; Zheng, Yi; +2 more

New TSPO Crystal Structures of Mutant and Heme-Bound Forms with Altered Flexibility, Ligand Binding, and Porphyrin Degradation Activity

Journal Article · Wed Mar 22 00:00:00 EDT 2023 · Biochemistry · OSTI ID:1169354

Liu, Jian; Hiser, Carrie; Li, Fei; +3 more

Correction to “New TSPO Crystal Structures of Mutant and Heme-Bound Forms with Altered Flexibility, Ligand Binding, and Porphyrin Degradation Activity”

Journal Article · Tue May 16 00:00:00 EDT 2023 · Biochemistry · OSTI ID:1169354

Liu, Jian; Hiser, Carrie; Li, Fei; +3 more

Title: Crystal structures of translocator protein (TSPO) and mutant mimic of a human polymorphism

Citation Formats

References (38)

Cited By (37)

Similar Records

Related Subjects