Supramolecular assembly of multifunctional maspin-mimetic nanostructures as a potent peptide-based angiogenesis inhibitor

Zha, R. Helen; Sur, Shantanu; Boekhoven, Job; Shi, Heidi Y.; Zhang, Ming; Stupp, Samuel I.

doi:10.1016/j.actbio.2014.11.001

Supramolecular assembly of multifunctional maspin-mimetic nanostructures as a potent peptide-based angiogenesis inhibitor

Journal Article · Sat Nov 08 00:00:00 EST 2014 · Acta Biomaterialia

DOI:https://doi.org/10.1016/j.actbio.2014.11.001· OSTI ID:1168848

Zha, R. Helen ^[1]; Sur, Shantanu ^[2]; Boekhoven, Job ^[2]; Shi, Heidi Y. ^[3]; Zhang, Ming ^[3]; Stupp, Samuel I. ^[4]

Northwestern Univ., Evanston, IL (United States). Dept. of Materials Science and Engineering; Northwestern Univ., Evanston, IL (United States). Simpson Querrey Inst. for BioNanotechnology
Northwestern Univ., Evanston, IL (United States). Simpson Querrey Inst. for BioNanotechnology
Northwestern Univ., Evanston, IL (United States). Dept. of Molecular Pharmacology and Biological Chemistry
Northwestern Univ., Evanston, IL (United States). Dept. of Materials Science and Engineering; Northwestern Univ., Evanston, IL (United States). Dept. of Chemistry; Northwestern Univ., Evanston, IL (United States). Simpson Querrey Inst. for BioNanotechnology; Northwestern Univ., Evanston, IL (United States). Dept. of Medicine

Aberrant angiogenesis plays a large role in pathologies ranging from tumor growth to macular degeneration. Anti-angiogenic proteins have thus come under scrutiny as versatile, potent therapeutics but face problems with purification and tissue retention. We report here on the synthesis of supramolecular nanostructures that mimic the anti-angiogenic activity of maspin, a class II tumor suppressor protein. These maspin-mimetic nanostructures are formed via self-assembly of small peptide amphiphiles containing the g-helix motif of maspin. Using tubulogenesis assays with human umbilical vein endothelial cells, we demonstrate that maspin-mimetic nanostructures show anti-angiogenic activity at concentrations that are significantly lower than those necessary for the g-helix peptide. Furthermore, in vivo assays in the chick chorioallantoic membrane show maspin-mimetic nanostructures to be effective over controls at inhibiting angiogenesis. Thus, in conclusion, the nanostructures investigated here offer an attractive alternative to the use of anti-angiogenic recombinant proteins in the treatment of cancer or other diseases involving abnormal blood vessel formation.

View Accepted Manuscript (DOE)

Research Organization:: Advanced Photon Source (APS), Argonne National Laboratory (ANL), Argonne, IL (US)

Sponsoring Organization:: National Cancer Institute (NCI); National Institutes of Health (NIH); USDOE; USDOE Office of Science (SC)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1168848

Alternate ID(s):: OSTI ID: 1251696

Journal Information:: Acta Biomaterialia, Journal Name: Acta Biomaterialia Journal Issue: C Vol. 12; ISSN 1742-7061

Publisher:: Acta Materialia, Inc.Copyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Aqueous self-assembly of short hydrophobic peptides containing norbornene amino acid into supramolecular structures with spherical shape Ruffoni, Alessandro; Cavanna, Maria V.; Argentiere, Simona RSC Advances, Vol. 6, Issue 93 https://doi.org/10.1039/c6ra17116h	journal	January 2016
Drug delivery by supramolecular design Webber, Matthew J.; Langer, Robert Chemical Society Reviews, Vol. 46, Issue 21 https://doi.org/10.1039/c7cs00391a	journal	January 2017

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59 BASIC BIOLOGICAL SCIENCES
anti-angiogenic
g-Helix
maspin
peptide amphiphile
self-assembly

Supramolecular assembly of multifunctional maspin-mimetic nanostructures as a potent peptide-based angiogenesis inhibitor

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