Viruslike Nanoparticles with Maghemite Cores Allow for Enhanced MRI Contrast Agents

Malyutin, Andrey G.; Easterday, Rosemary; Lozovyy, Yaroslav; Spilotros, Alessandro; Cheng, Hu; Sanchez-Felix, Olivia R.; Stein, Barry D.; Morgan, David Gene; Svergun, Dmitri I.; Dragnea, Bogdan; Bronstein, Lyudmila M.

doi:10.1021/cm504029j

Title: Viruslike Nanoparticles with Maghemite Cores Allow for Enhanced MRI Contrast Agents

Journal Article · Tue Dec 23 00:00:00 EST 2014 · Chemistry of Materials

DOI:https://doi.org/10.1021/cm504029j· OSTI ID:1167524

Malyutin, Andrey G. ^[1]; Easterday, Rosemary ^[1]; Lozovyy, Yaroslav ^[1]; Spilotros, Alessandro ^[2]; Cheng, Hu ^[3]; Sanchez-Felix, Olivia R. ^[1]; Stein, Barry D. ^[4]; Morgan, David Gene ^[1]; Svergun, Dmitri I. ^[2]; Dragnea, Bogdan ^[1]; Bronstein, Lyudmila M. ^[5]

Department of Chemistry, Indiana University, 800 East Kirkwood Avenue, Bloomington, Indiana 47405, United States
European Molecular Biology Laboratory (EMBL), Hamburg Outstation, Notkestraße 85, D-22603 Hamburg, Germany
Department of Psychological and Brain Sciences, Indiana University, 1101 East Tenth Street, Bloomington, Indiana 47403, United States
Department of Biology, Indiana University, 1001 East Third Street, Bloomington, Indiana 47405, United States
Department of Chemistry, Indiana University, 800 East Kirkwood Avenue, Bloomington, Indiana 47405, United States, Department of Physics, Faculty of Science , King Abdulaziz University, Jeddah, Saudi Arabia

Here, for the first time, we demonstrate formation of virus-like nanoparticles (VNPs) utilizing gold-coated iron oxide nanoparticles as cores and capsidprotein of brome mosaic virus (BMV) or hepatitis B virus (HBV) as shells. Further, utilizing cryo-electron microscopy and single particle methods, we are able to show that the BMV coat on VNPs assembles into a structure very close to that of a native virion. This is a consequence of an optimal iron oxide NP size (~11 nm) fitting the virus cavity and an ultrathin gold layer on the maghemite cores, which allows for utilization of SH-(CH₂)₁₁-(CH₂-CH₂-O)₄-OCH₂-COOH as capping molecules to provide sufficient stability, charge density, and small form factor. MRI studies show unique relaxivity ratios that diminish only slightly with gold coating. In conclusion, a virus protein coating of a magnetic core mimicking the wild-type virus makes these VNPs a versatile platform for biomedical applications.

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Research Organization:: Indiana Univ., Bloomington, IN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0010507

OSTI ID:: 1167524

Alternate ID(s):: OSTI ID: 1440972

Journal Information:: Chemistry of Materials, Journal Name: Chemistry of Materials Vol. 27 Journal Issue: 1; ISSN 0897-4756

Publisher:: American Chemical SocietyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 28 works

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