Pyrococcus furiosus strains and methods of using same

Lipscomb, Gina L; Farkas, Joel Andrew; Adams, Michael W. W.; Westpheling, Janet

Pyrococcus furiosus strains and methods of using same

Patent · Tue Jan 06 04:00:00 EST 2015

OSTI ID:1167026

Lipscomb, Gina L; Farkas, Joel Andrew; Adams, Michael W. W.; Westpheling, Janet

Provided herein are methods for transforming a Pyrococcus furiosus with a polynucleotide. In one embodiment, the method includes contacting a P. furiosus with a polynucleotide under conditions suitable for uptake of the polynucleotide by the P. furiosus, and identifying transformants at a frequency of, for instance, at least 10.sup.3 transformants per microgram DNA. Also provided are isolated Pyrococcus furiosus having the characteristics of Pyrococcus furiosus COM1, and plasmids that include an origin of replication that functions in a Pyrococcus furiosus. The plasmid is stable in a recipient P. furiosus without selection for more than 100 generations and is structurally unchanged after replication in P. furiosus for more than 100 generations.

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Research Organization:: University of Georgia Research Foundation, Inc., Athens, GA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG05-95ER20175

Assignee:: University of Georgia Research Foundation, Inc. (Athens, GA)

Patent Number(s):: 8,927,254

Application Number:: 13/248,377

OSTI ID:: 1167026

Country of Publication:: United States

Language:: English

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