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Extraction of Sulfur Mustard Metabolites from Urine Samples and Analysis by Liquid Chromatography-High-Resolution Mass Spectrometry (LC-HRMS)

Technical Report ·
DOI:https://doi.org/10.2172/1165796· OSTI ID:1165796
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  1. Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

The following procedure describes the extraction of sulfur mustard metabolites (principally 1,1’-sulfonylbis[2-(methylthio)ethane] (SBMTE)) and related chemicals from urine and subsequent analysis by liquid chromatography with detection by high resolution mass spectrometry (LC-HRMS) to determine signatures present in a contaminated urine sample. Positive identification of such signatures in urine may signify patient exposure to sulfur mustard (HD). A future SOP will detail analysis of these metabolites by gas chromatography-tandem mass spectrometry (GC-MS/MS).

Research Organization:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Sponsoring Organization:
USDOE National Nuclear Security Administration (NNSA)
DOE Contract Number:
AC52-07NA27344
OSTI ID:
1165796
Report Number(s):
LLNL--TR-659681
Country of Publication:
United States
Language:
English