Preservation of H 2 production activity in nanoporous latex coatings of Rhodopseudomonas palustris CGA009 during dry storage at ambient temperatures: Preservation of R.palustris latex coatings

Piskorska, M.; Soule, T.; Gosse, J. L.; Milliken, C.; Flickinger, M. C.; Smith, G. W.; Yeager, C. M.

doi:10.1111/1751-7915.12032

Title: Preservation of H ₂ production activity in nanoporous latex coatings of Rhodopseudomonas palustris CGA009 during dry storage at ambient temperatures: Preservation of R.palustris latex coatings

Journal Article · Sun Jul 21 00:00:00 EDT 2013 · Microbial Biotechnology (Online)

DOI:https://doi.org/10.1111/1751-7915.12032· OSTI ID:1163478

Piskorska, M. ^[1]; Soule, T. ^[2]; Gosse, J. L. ^[3]; Milliken, C. ^[2]; Flickinger, M. C. ^[3]; Smith, G. W. ^[1]; Yeager, C. M. ^[2]

Univ. of South Carolina, Aiken, SC (United States)
Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL)
North Carolina State Univ., Raleigh, NC (United States)

To assess the applicability of latex cell coatings as an ‘off-the-shelf’ biocatalyst, the effect of osmoprotectants, temperature, humidity and O₂ on preservation of H₂ production in Rhodopseudomonas palustris coatings was evaluated. Immediately following latex coating coalescence (24 h) and for up to 2 weeks of dry storage, rehydrated coatings containing different osmoprotectants displayed similar rates of H₂ production. Beyond 2 weeks of storage, sorbitol-treated coatings lost all H₂ production activity, whereas considerable H₂ production was still detected in sucrose- and trehalose-stabilized coatings. We stored the coatings at a relative humidity level which significantly impacts the recovery and subsequent rates of H₂ production. After 4 weeks storage under air at 60% humidity, coatings produced only trace amounts of H₂ (0–0.1% headspace accumulation), whereas those stored at < 5% humidity retained 27–53% of their H₂ production activity after 8 weeks of storage. Furthermore, when stored in argon at < 5% humidity and room temperature, R. palustris coatings retained full H₂ production activity for 3 months, implicating oxidative damage as a key factor limiting coating storage. Ultimately, the results demonstrate that biocatalytic latex coatings are an attractive cell immobilization platform for preservation of bioactivity in the dry state.

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Research Organization:: Univ. of South Carolina, Columbia, SC (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office; USDOE Office of Environmental Management (EM)

Grant/Contract Number:: EE0003152

OSTI ID:: 1163478

Report Number(s):: DOE-USCA-3152-2

Journal Information:: Microbial Biotechnology (Online), Vol. 6, Issue 5; ISSN 1751-7915

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 13 works

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Title: Preservation of H ₂ production activity in nanoporous latex coatings of Rhodopseudomonas palustris CGA009 during dry storage at ambient temperatures: Preservation of R.palustris latex coatings