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Process and genes for expression and overexpression of active [FeFe] hydrogenases

Patent ·
OSTI ID:1159827
; ; ; ;
A process for expression of active [FeFe]-hydrogenase in a host organism that does not contain either the structural gene(s) for [FeFe]-hydrogenases and/or homologues for the maturation genes HydE, HydF and HyG, comprising: cloning the structural hydrogenase gene(s) and/or the maturation genes HydE, HydF and HydG from an organisms that contains these genes into expression plasmids; transferring the plasmids into an organism that lacks a native [FeFe]-hydrogenase or that has a disrupted [FeFe]-hydrogenase and culturing it aerobically; and inducing anaerobiosis to provide [FeFe] hydrogenase biosynthesis and H?2#191 production.
Research Organization:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC36-99GO10337
Assignee:
Alliance for Sustainable Energy, LLC (Golden, CO)
Patent Number(s):
8,835,153
Application Number:
11/573,035
OSTI ID:
1159827
Country of Publication:
United States
Language:
English

References (18)

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Functional Studies of [FeFe] Hydrogenase Maturation in an Escherichia coli Biosynthetic System journal March 2006
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Purification and characterization of Desulfovibrio vulgaris (Hildenborough) hydrogenase expressed in Escherichia coli journal January 1987
Discovery of Two Novel Radical S -Adenosylmethionine Proteins Required for the Assembly of an Active [Fe] Hydrogenase journal April 2004
Isolation of Genes Required for Hydrogenase Synthesis in Escherichia coli journal December 1987
Expression of two [Fe]-hydrogenases in Chlamydomonas reinhardtii under anaerobic conditions: Expression of a second [Fe]-hydrogenase in C. reinhardtii journal June 2003
Microalgae: a green source of renewable H2 journal December 2000
Characterization of a [2Fe-2S] protein encoded in the iron-hydrogenase operon of Thermotoga maritima journal February 2003
Maturation of the [NiFe] hydrogenases journal May 2001
Initial cloning and sequencing of hydHG, an operon homologous to ntrBC and regulating the labile hydrogenase activity in Escherichia coli K-12. journal August 1989
Classification and phylogeny of hydrogenases journal August 2001
The hyp operon gene products are required for the maturation of catalytically active hydrogenase isoenzymes in Escherichia coli journal November 1992
High-frequency nuclear transformation of Chlamydomonas reinhardtii. journal February 1990
Iron-Dependent Hydrogenases of Entamoeba histolytica and Giardia lamblia: Activity of the Recombinant Entamoebic Enzyme and Evidence for Lateral Gene Transfer journal February 2003

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59 BASIC BIOLOGICAL SCIENCES