Characterisation of the LH2 spectral variants produced by the photosynthetic purple sulphur bacterium Allochromatium vinosum

Carey, Anne-Marie; Hacking, Kirsty; Picken, Nichola; Honkanen, Suvi; Kelly, Sharon; Niedzwiedzki, Dariusz M.; Blankenship, Robert E.; Shimizu, Yuuki; Wang-Otomo, Zheng-Yu; Cogdell, Richard J.

doi:10.1016/j.bbabio.2014.07.022

Characterisation of the LH2 spectral variants produced by the photosynthetic purple sulphur bacterium Allochromatium vinosum

Journal Article · Sat Nov 01 04:00:00 EDT 2014 · Biochimica et Biophysica Acta - Bioenergetics

DOI:https://doi.org/10.1016/j.bbabio.2014.07.022· OSTI ID:1150020

Carey, Anne-Marie ^[1]; Hacking, Kirsty; Picken, Nichola; Honkanen, Suvi; Kelly, Sharon; Niedzwiedzki, Dariusz M.; Blankenship, Robert E.; Shimizu, Yuuki; Wang-Otomo, Zheng-Yu; Cogdell, Richard J.

Washington University in St. Louis

This study systematically investigated the different types of LH2 produced by Allochromatium (Alc.) vinosum, a photosynthetic purple sulphur bacterium, in response to variations in growth conditions. Three different spectral forms of LH2 were isolated and purified, the B800-820, B800-840 and B800-850 LH2 types, all of which exhibit an unusual split 800 peak in their low temperature absorption spectra. However, it is likely that more forms are also present. Relatively more B800-820 and B800-840 are produced under low light conditions, while relatively more B800-850 is produced under high light conditions. Polypeptide compositions of the three different LH2 types were determined by a combination of HPLC and TOF/MS. The B800-820, B800-840 and B800-850 LH2 types all have a heterogeneous polypeptide composition, containing multiple types of both α and β polypeptides, and differ in their precise polypeptide composition. They all have a mixed carotenoid composition, containing carotenoids of the spirilloxanthin series. In all cases the most abundant carotenoid is rhodopin; however, there is a shift towards carotenoids with a higher conjugation number in LH2 complexes produced under low light conditions. CD spectroscopy, together with the polypeptide analysis, demonstrates that these Alc. vinosum LH2 complexes are more closely related to the LH2 complex from Phs. molischianum than they are to the LH2 complexes from Rps. acidophila.

Research Organization:: Washington Univ., St. Louis, MO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

DOE Contract Number:: SC0001035

OSTI ID:: 1150020

Report Number(s):: DE-SC0001035-121

Journal Information:: Biochimica et Biophysica Acta - Bioenergetics, Journal Name: Biochimica et Biophysica Acta - Bioenergetics Journal Issue: 11 Vol. 1837; ISSN 0005-2728

Publisher:: Elsevier

Country of Publication:: United States

Language:: English

References (54)

Spectroscopic Determination of Tryptophan and Tyrosine in Proteins ^* Edelhoch, Harold Biochemistry, Vol. 6, Issue 7 https://doi.org/10.1021/bi00859a010	journal	July 1967
Tracking energy transfer between light harvesting complex 2 and 1 in photosynthetic membranes grown under high and low illumination Luer, L.; Moulisova, V.; Henry, S. Proceedings of the National Academy of Sciences, Vol. 109, Issue 5 https://doi.org/10.1073/pnas.1113080109	journal	January 2012
Differentiation of the photosynthetic apparatus of Chromatium vinosum, strain D: I. The influence of growth conditions Mechler, B.; Oelze, J. Archives of Microbiology, Vol. 118, Issue 1 https://doi.org/10.1007/BF00406080	journal	January 1978
Methionine oxidation and its effect on the stability of a reconstituted subunit of the light-harvesting complex from Rhodospirillum rubrum : Methionine oxidation and its effect on LH1 subunit Wang, Zheng-Yu; Shimonaga, Masahiro; Muraoka, Yoshiyuki European Journal of Biochemistry, Vol. 268, Issue 12 https://doi.org/10.1046/j.1432-1327.2001.02234.x	journal	June 2001
Fluorescence of native and carotenoid-depleted LH2 from Chromatium minutissimum , originating from simultaneous two-photon absorption in the spectral range of the presumed (optically ‘dark’) S ₁ state of carotenoids Krikunova, M.; Kummrow, A.; Voigt, B. FEBS Letters, Vol. 528, Issue 1-3 https://doi.org/10.1016/S0014-5793(02)03315-X	journal	September 2002
The effect of growth conditions on the light-harvesting apparatus in Rhodopseudomonas acidophila Gardiner, Alastair T.; Cogdell, Richard J.; Takaichi, Shinichi Photosynthesis Research, Vol. 38, Issue 2 https://doi.org/10.1007/BF00146415	journal	November 1993
Ultrafast time-resolved spectroscopy of the light-harvesting complex 2 (LH2) from the photosynthetic bacterium Thermochromatium tepidum Niedzwiedzki, Dariusz M.; Fuciman, Marcel; Kobayashi, Masayuki Photosynthesis Research, Vol. 110, Issue 1 https://doi.org/10.1007/s11120-011-9692-7	journal	October 2011
Spectroscopic studies of two spectral variants of light-harvesting complex 2 (LH2) from the photosynthetic purple sulfur bacterium Allochromatium vinosum Niedzwiedzki, Dariusz M.; Bina, David; Picken, Nichola Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1817, Issue 9 https://doi.org/10.1016/j.bbabio.2012.05.009	journal	September 2012
Structural and spectral characterisation of the antenna complexes of Rhodocyclus gelatinosus. Indications of a hairpin-like-arranged antenna apoprotein with an unusually high alanine content Brunisholz, Rene A.; Suter, Franz; Zuber, Herbert European Journal of Biochemistry, Vol. 222, Issue 2 https://doi.org/10.1111/j.1432-1033.1994.tb18911.x	journal	June 1994
The crystal structure of the light-harvesting complex II (B800–850) from Rhodospirillum molischianum Koepke, Juergen; Hu, Xiche; Muenke, Cornelia Structure, Vol. 4, Issue 5 https://doi.org/10.1016/S0969-2126(96)00063-9	journal	May 1996
Role of the PufB and PufA C-Terminal Extensions in the Assembly of Rhodospirillum rubrum Light-Harvesting Antenna Butzin, Nicholas C.; A. Benson, Jodi; Merchant, Daniel J. Current Microbiology, Vol. 60, Issue 4 https://doi.org/10.1007/s00284-009-9541-5	journal	November 2009
Red Shift of the Zero Crossing in the CD Spectra of the LH2 Antenna Complex of Rhodopseudomonas acidophila : A Structure-Based Study Koolhaas, M. H. C.; van der Zwan, G.; Frese, R. N. The Journal of Physical Chemistry B, Vol. 101, Issue 37 https://doi.org/10.1021/jp963368k	journal	September 1997
The Structure and Thermal Motion of the B800–850 LH2 Complex from Rps.acidophila at 2.0Å Resolution and 100K: New Structural Features and Functionally Relevant Motions Papiz, Miroslav Z.; Prince, Steve M.; Howard, Tina Journal of Molecular Biology, Vol. 326, Issue 5 https://doi.org/10.1016/S0022-2836(03)00024-X	journal	March 2003
Sub-picosecond excited-state dynamics of a carotenoid (spirilloxanthin) in the light-harvesting systems of Chromatium vinosum. Okamoto, Hiromi; Ogura, Mikio; Nakabayashi, Takakazu Chemical Physics, Vol. 236, Issue 1-3 https://doi.org/10.1016/S0301-0104(98)00207-9	journal	September 1998
Spectroscopy and structure of bacteriochlorophyll dimers. I. Structural consequences of nonconservative circular dichroism spectra Koolhaas, M. H.; van der Zwan, G.; van Mourik, F. Biophysical Journal, Vol. 72, Issue 4 https://doi.org/10.1016/S0006-3495(97)78829-5	journal	April 1997
Femtosecond Spectroscopy of Native and Carotenoidless Purple-Bacterial LH2 Clarifies Functions of Carotenoids Theiss, Christoph; Leupold, Dieter; Moskalenko, Andrei A. Biophysical Journal, Vol. 94, Issue 12 https://doi.org/10.1529/biophysj.107.121681	journal	June 2008
Two-dimensional structure of the native light-harvesting complex LH2 from Rubrivivax gelatinosus and of a truncated form Ranck, Jean-Luc; Ruiz, Teresa; Péhau-Arnaudet, Gérard Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1506, Issue 1 https://doi.org/10.1016/S0005-2728(01)00185-2	journal	July 2001
Energy Transfer and Exciton Coupling in Isolated B800−850 Complexes of the Photosynthetic Purple Sulfur Bacterium Chromatium tepidum . The Effect of Structural Symmetry on Bacteriochlorophyll Excited States Kennis, John T. M.; Streltsov, Alexander M.; Aartsma, Thijs J. The Journal of Physical Chemistry, Vol. 100, Issue 6 https://doi.org/10.1021/jp952475w	journal	January 1996
Femtosecond Dynamics in Isolated LH2 Complexes of Various Species of Purple Bacteria ^‖ Kennis, John T. M.; Streltsov, Alexandre M.; Vulto, Simone I. E. The Journal of Physical Chemistry B, Vol. 101, Issue 39 https://doi.org/10.1021/jp963359b	journal	September 1997
Purification and Characterization of the Polypeptides of Core Light-Harvesting Complexes from Purple Sulfur Bacteria Wang, Zheng-Yu; Shimonaga, Masahiro; Suzuki, Hiroaki Photosynthesis Research, Vol. 78, Issue 2 https://doi.org/10.1023/B:PRES.0000004328.11219.79	journal	January 2003
Absorption and CD Spectroscopy and Modeling of Various LH2 Complexes from Purple Bacteria Georgakopoulou, Sofia; Frese, Raoul N.; Johnson, Evelyn Biophysical Journal, Vol. 82, Issue 4 https://doi.org/10.1016/S0006-3495(02)75565-3	journal	April 2002
Heterogeneity of carotenoid content and composition in LH2 of the purple sulphur bacterium Allochromatium minutissimum grown under carotenoid-biosynthesis inhibition Makhneva, Zoya; Bolshakov, Maksim; Moskalenko, Andrey Photosynthesis Research, Vol. 98, Issue 1-3 https://doi.org/10.1007/s11120-008-9384-0	journal	October 2008
Crystal structure of an integral membrane light-harvesting complex from photosynthetic bacteria McDermott, G.; Prince, S. M.; Freer, A. A. Nature, Vol. 374, Issue 6522 https://doi.org/10.1038/374517a0	journal	April 1995
Antenna organization of Rhodopseudomonas acidophila: a study of the excitation migration Deinum, Geurt; Otte, Stephan C. M.; Gardiner, Alastair T. Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1060, Issue 1 https://doi.org/10.1016/S0005-2728(05)80127-6	journal	September 1991
Genetically modified photosynthetic antenna complexes with blueshifted absorbance bands Fowler, G. J. S.; Visschers, R. W.; Grief, G. G. Nature, Vol. 355, Issue 6363 https://doi.org/10.1038/355848a0	journal	February 1992
Picosecond time-resolved resonance Raman scattering and absorbance changes from carotenoids in light-harvesting systems of photosynthetic bacterium Chromatium vinosum Hayashi, H.; Kolaczkowski, S. V.; Noguchi, T. Journal of the American Chemical Society, Vol. 112, Issue 12 https://doi.org/10.1021/ja00168a006	journal	June 1990
Near-Infrared Absorption Spectra of Light Harvesting Bacteriochlorophyll Protein Complexes from Chromatium vinosum1 Hayashi, Hidenori; Morita, Sigehiro The Journal of Biochemistry, Vol. 88, Issue 5 https://doi.org/10.1093/oxfordjournals.jbchem.a133093	journal	October 1980
Reconstitution of Carotenoids into the Light-Harvesting Complex B800–850 of Chromatium minutissimum Toropygina, O. A.; Makhneva, Z. K.; Moskalenko, A. A. Biochemistry (Moscow), Vol. 68, Issue 8, p. 901-911 https://doi.org/10.1023/A:1025755116593	journal	January 2003
Differentiation of the photosynthetic apparatus of Chromatium vinosum, strain D: III. Analyses of spectral alterations Mechler, B.; Oelze, J. Archives of Microbiology, Vol. 118, Issue 1 https://doi.org/10.1007/BF00406082	journal	January 1978
Isolation and characterization of a subunit form of the light-harvesting complex of Rhodospirillum rubrum Miller, J. F.; Hinchigeri, S. B.; Parkes-Loach, P. S. Biochemistry, Vol. 26, Issue 16 https://doi.org/10.1021/bi00390a026	journal	August 1987
Crystallization and preliminary X-ray crystallographic analysis of the B800–820 light-harvesting complex from Rhodopseudomonas acidophila strain 7050 McLuskey, Karen; Prince, Stephen M.; Cogdell, Richard J. Acta Crystallographica Section D Biological Crystallography, Vol. 55, Issue 4 https://doi.org/10.1107/S0907444998016059	journal	April 1999
X-Ray Diffraction Studies on Chromatophore Membrane from Photosynthetic Bacteria. III. Basic Structure of the Photosynthetic Unit and Its Relation to Other Bacteriochlorophyll Forms1 Nakamoto, Shinya; Kataoka, Mikio; Ueki, Tatzuo The Journal of Biochemistry, Vol. 96, Issue 6 https://doi.org/10.1093/oxfordjournals.jbchem.a135017	journal	October 1984
Reconstitution of Okenone into Light Harvesting Complexes from Allochromatium minutissimum Toropygina, O. A.; Makhneva, Z. K.; Moskalenko, A. A. Biochemistry (Moscow), Vol. 70, Issue 11 https://doi.org/10.1007/s10541-005-0252-7	journal	November 2005
The architecture and function of the light-harvesting apparatus of purple bacteria: from single molecules to in vivo membranes Cogdell, Richard J.; Gall, Andrew; Köhler, Jürgen Quarterly Reviews of Biophysics, Vol. 39, Issue 3 https://doi.org/10.1017/S0033583506004434	journal	August 2006
Complete genome sequence of Allochromatium vinosum DSM 180T Weissgerber, Thomas; Zigann, Renate; Bruce, David Standards in Genomic Sciences, Vol. 5, Issue 3 https://doi.org/10.4056/sigs.2335270	journal	December 2011
Gene sequencing and characterization of the light-harvesting complex 2 from thermophilic purple sulfur bacterium Thermochromatium tepidum Sekine, Fumie; Horiguchi, Kentaro; Kashino, Yasuhiro Photosynthesis Research, Vol. 111, Issue 1-2 https://doi.org/10.1007/s11120-011-9658-9	journal	May 2011
The light intensity under which cells are grown controls the type of peripheral light-harvesting complexes that are assembled in a purple photosynthetic bacterium Brotosudarmo, Tatas H. P.; Collins, Aaron M.; Gall, Andrew Biochemical Journal, Vol. 440, Issue 1 https://doi.org/10.1042/BJ20110575	journal	October 2011
Two-Photon Excitation Spectroscopy of Carotenoid-Containing and Carotenoid-Depleted LH2 Complexes from Purple Bacteria Stepanenko, Ilya; Kompanetz, Viktor; Makhneva, Zoya The Journal of Physical Chemistry B, Vol. 113, Issue 34 https://doi.org/10.1021/jp906565m	journal	August 2009
Circular Dichroism of Bacteriochlorophyll α in Light Harvesting Bacteriochlorophyll Protein Complexes from Chromatium vinosum1 Hayashi, Hidenori; Nozawa, Tsunenori; Hatano, Masahiro The Journal of Biochemistry, Vol. 89, Issue 6 https://doi.org/10.1093/oxfordjournals.jbchem.a133387	journal	April 1981
Light-induced blue shift of the carotenoid spectrum in chromatophores of Chromatium vinosum strain D Bowyer, John R.; Crofts, Antony R. Archives of Biochemistry and Biophysics, Vol. 202, Issue 2 https://doi.org/10.1016/0003-9861(80)90465-8	journal	July 1980
Light-harvesting complexes from purple sulfur bacteria Allochromatium minutissimum assembled without carotenoids Moskalenko, A. A.; Makhneva, Z. K. Journal of Photochemistry and Photobiology B: Biology, Vol. 108 https://doi.org/10.1016/j.jphotobiol.2011.11.006	journal	March 2012
Formation of Bacteriochlorophyll Form B820 in Light Harvesting 2 Complexes from Purple Sulfur Bacteria Treated with Dioxane Makhneva, Z. K.; Toropygina, O. A.; Moskalenko, A. A. Biochemistry (Moscow), Vol. 70, Issue 10 https://doi.org/10.1007/s10541-005-0233-x	journal	October 2005
Differentiation of the photosynthesic apparatus of Chromatium vinosum, strain D: II. Structural and functional differences Mechler, B.; Oelze, J. Archives of Microbiology, Vol. 118, Issue 1 https://doi.org/10.1007/BF00406081	journal	January 1978
The primary structure of the antenna polypeptides of Ectothiorhodospira halochloris and Ectothiorhodospira halophila. Four core-type antenna polypeptides in E. halochtoris and E. halophila Wagner-Huber, Regula; Brunisholz, Rene A.; Bissig, Iwan European Journal of Biochemistry, Vol. 205, Issue 3 https://doi.org/10.1111/j.1432-1033.1992.tb16858.x	journal	May 1992
The peripheral light-harvesting complexes from purple sulfur bacteria have different ‘ring’ sizes Kereïche, Sami; Bourinet, Laurent; Keegstra, Wilko FEBS Letters, Vol. 582, Issue 25-26 https://doi.org/10.1016/j.febslet.2008.09.050	journal	October 2008
Uphill energy transfer in LH2-containing purple bacteria at room temperature Trissl, Hans-Wilhelm; Law, Christopher J.; Cogdell, Richard J. Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1412, Issue 2 https://doi.org/10.1016/S0005-2728(99)00056-0	journal	June 1999
A modified method for the cultivation of phototrophic bacteria Malik, K. A. Journal of Microbiological Methods, Vol. 1, Issue 6 https://doi.org/10.1016/0167-7012(83)90011-8	journal	December 1983
Properties of Chromatium Subchromatophore Particles Obtained by Treatment with Triton X-100 ^* Garcia, Augusto; Vernon, Leo P.; Mollenhauer, Hilton Biochemistry, Vol. 5, Issue 7 https://doi.org/10.1021/bi00871a033	journal	July 1966
The light-harvesting complexes of a thermophilic purple sulfur photosynthetic bacterium Chromatium tepidum Garcia, D.; Parot, P.; Verméglio, A. Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 850, Issue 2 https://doi.org/10.1016/0005-2728(86)90195-7	journal	July 1986
Photochemical reactions of purple bacteria as revealed by studies of three spectrally different carotenobacteriochlorophyll-protein complexes isolated from Chromatium, strain D Thornber, J. Philip; Sokoloff, Miriam K. Biochemistry, Vol. 9, Issue 13 https://doi.org/10.1021/bi00815a017	journal	June 1970
The Crystallographic Structure of the B800-820 LH3 Light-Harvesting Complex from the Purple Bacteria Rhodopseudomonas Acidophila Strain 7050 ^† McLuskey, K.; Prince, S. M.; Cogdell, R. J. Biochemistry, Vol. 40, Issue 30 https://doi.org/10.1021/bi010309a	journal	July 2001
Single-Molecule Spectroscopy Reveals that Individual Low-Light LH2 Complexes from Rhodopseudomonas palustris 2.1.6. Have a Heterogeneous Polypeptide Composition Brotosudarmo, Tatas H. P.; Kunz, Ralf; Böhm, Paul Biophysical Journal, Vol. 97, Issue 5 https://doi.org/10.1016/j.bpj.2009.06.034	journal	September 2009
Absorption bands of bacteriochlorophyll types in purple bacteria and their response to illumination Vredenberg, W. J.; Amesz, J. Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis, Vol. 126, Issue 2 https://doi.org/10.1016/0926-6585(66)90060-4	journal	October 1966
Deletion of a B800−850 Light-Harvesting Complex in Rhodospirillum molischianum DSM119 Leads to “Revertants” Expressing a B800−820 Complex: Insights into Pigment Binding ^† Sauer, Philippe R. R.; Lottspeich, Friedrich; Unger, Esko Biochemistry, Vol. 35, Issue 20 https://doi.org/10.1021/bi9528255	journal	January 1996

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