Assignment of human {alpha}-synuclein (SNCA) and {beta}-synuclein (SNCB) genes to chromosomes 4q21 and 5q35

Journal Article · Sat May 20 04:00:00 EDT 1995 · Genomics

DOI:https://doi.org/10.1006/geno.1995.1063· OSTI ID:114854

Spillantini, M G; Goedert, M ^[1]; Divane, A ^[2]

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1. MRC Lab. of Molecular Biology, Cambridge (United Kingdom)
2. Univ. of Cambridge (United Kingdom)

The authors previously identified two human brain proteins of 140 and 134 amino acids by virtue of their reactivity with a monoclonal antibody raised against a tangle preparation from Alzheimer disease brain. The 140-amino-acid protein is homologous to synuclein from Torpedo electroplaques and rat brain and identical to the precursor of the non-A{beta} component of Alzheimer disease amyloid plaques. The 134-amino-acid protein is homologous to bovine phosphoneuroprotein 14. In view fo the fact that both proteins are 61% identical in sequence, they have called them {alpha}-synuclein and {beta}-synuclein, respectively. Both proteins are present in nerve terminals, where they may be involved in the events mediating exocytosis of synaptic vesicles following nerve stimulation. They have now determined the chromosomal localizations of the {alpha}-synuclein and {beta}-synuclein genes. To map the two genes analyzed a panel of monochromosomal human-rodent somatic cell hybride by polymerase chain reaction and performed fluorescence in situ hybridization on metaphase spreads f human chromosomes.

• OSTI ID: 114854
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 27; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BRAIN
DNA HYBRIDIZATION
GENES
GENETIC MAPPING
HUMAN CHROMOSOME 5
HUMAN CHROMOSOMES
POLYMERASE CHAIN REACTION