Cloning and chromosomal localization of the human A{sub 2b} adenosine receptor gene (ADORA2B) and its pseudogene
- Merck Research Labs., West Point, PA (United States); and others
To determine the chromosomal localization of the human A{sub 2b} adenosine receptor, the corresponding genomic clone was isolated and used as a probe for fluorescence in situ hybridization to metaphase chromosomes. Partial sequence analysis of the A{sub 2b} gene (ADORA2B) revealed an intron that interrupted the coding region corresponding to the second intracellular loop similar to that reported for A{sub 1} and A{sub 2a} adenosine receptor genes. A pseudogene for the A{sub 2b} receptor was also identified; it exhibited 79% identity to the A{sub 2b} adenosine receptor cDNA coding sequence and contained multiple deletions, point mutations, and frame shifts and two in-frame stops. These changes would result in the inability to encode a functional receptor. The genomic clones were utilized to localize the A{sub 2b} receptor to chromosome 17p12 and the A{sub 2b} pseudogene to chromosome 1q32. 15 refs., 1 fig.
- OSTI ID:
- 114852
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 27; ISSN 0888-7543; ISSN GNMCEP
- Country of Publication:
- United States
- Language:
- English
Similar Records
Molecular analysis of functional and nonfunctional genes for human ferrochelatase: Isolation and characterization of a FECH pseudogene and its sublocation on chromosome 3
The high-affinity interleukin 8 receptor gene (IL8RA) maps to the 2q33-q36 region of the human genome: Cloning of a pseudogene (IL8RBP) for the low-affinity receptor