skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: High-Throughput Biosensor Discriminates Between Different Algal H 2-Photoproducing Strains

Abstract

A number of species of microalgae and cyanobacteria photosynthetically produce H 2 gas by coupling water oxidation with the reduction of protons to molecular hydrogen, generating renewable energy from sunlight and water. Photosynthetic H 2 production, however, is transitory, and there is considerable interest in increasing and extending it for commercial applications. Here we report a Petri-plate version of our previous, microplate-based assay that detects photosynthetic H 2 production by algae. The assay consists of an agar overlay of H 2-sensing Rhodobacter capsulatus bacteria carrying a green fluorescent protein that responds to H 2 produced by single algal colonies in the bottom agar layer. The assay distinguishes between algal strains that photoproduce H 2 at different levels under high light intensities, and it does so in a simple, inexpensive, and high-throughput manner. The assay will be useful for screening both natural populations and mutant libraries for strains having increased H 2 production, and useful for identifying various genetic factors that physiologically or genetically alter algal hydrogen production.

Authors:
 [1];
  1. National Renewable Energy Lab. (NREL), Golden, CO (United States)
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Science Office of Biological and Environmental Research
OSTI Identifier:
1136212
DOE Contract Number:  
AC36-08GO28308
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biotechnology and Bioengineering; Journal Volume: 111; Journal Issue: 7, July 2014
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES; 08 HYDROGEN; Chemical and Biosciences

Citation Formats

Wecker, Matt S. A., and Maria L. Ghirardi. High-Throughput Biosensor Discriminates Between Different Algal H2-Photoproducing Strains. United States: N. p., 2014. Web. doi:10.1002/bit.25206.
Wecker, Matt S. A., & Maria L. Ghirardi. High-Throughput Biosensor Discriminates Between Different Algal H2-Photoproducing Strains. United States. doi:10.1002/bit.25206.
Wecker, Matt S. A., and Maria L. Ghirardi. Thu . "High-Throughput Biosensor Discriminates Between Different Algal H2-Photoproducing Strains". United States. doi:10.1002/bit.25206.
@article{osti_1136212,
title = {High-Throughput Biosensor Discriminates Between Different Algal H2-Photoproducing Strains},
author = {Wecker, Matt S. A. and Maria L. Ghirardi},
abstractNote = {A number of species of microalgae and cyanobacteria photosynthetically produce H2 gas by coupling water oxidation with the reduction of protons to molecular hydrogen, generating renewable energy from sunlight and water. Photosynthetic H2 production, however, is transitory, and there is considerable interest in increasing and extending it for commercial applications. Here we report a Petri-plate version of our previous, microplate-based assay that detects photosynthetic H2 production by algae. The assay consists of an agar overlay of H2-sensing Rhodobacter capsulatus bacteria carrying a green fluorescent protein that responds to H2 produced by single algal colonies in the bottom agar layer. The assay distinguishes between algal strains that photoproduce H2 at different levels under high light intensities, and it does so in a simple, inexpensive, and high-throughput manner. The assay will be useful for screening both natural populations and mutant libraries for strains having increased H2 production, and useful for identifying various genetic factors that physiologically or genetically alter algal hydrogen production.},
doi = {10.1002/bit.25206},
journal = {Biotechnology and Bioengineering},
number = 7, July 2014,
volume = 111,
place = {United States},
year = {Thu Feb 27 00:00:00 EST 2014},
month = {Thu Feb 27 00:00:00 EST 2014}
}