The role of photo-osmotic adaptation in semi-continuous culture and lipid particle release from Dunaliella viridis

Davis, Ryan W.; Carvalho, Benjamin J.; Jones, Howland D. T.; Singh, Seema

doi:10.1007/s10811-014-0331-5

The role of photo-osmotic adaptation in semi-continuous culture and lipid particle release from Dunaliella viridis

Journal Article · Tue May 13 00:00:00 EDT 2014 · Journal of Applied Phycology

DOI:https://doi.org/10.1007/s10811-014-0331-5· OSTI ID:1122967

Davis, Ryan W. ^[1]; Carvalho, Benjamin J. ^[1]; Jones, Howland D. T. ^[2]; Singh, Seema ^[1]

Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

Great efforts have been made to elucidate the phenotypic responses of alga to varying levels of nutrients, osmotic environments, and photosynthetically active radiation intensities, though the role of interactions among these variables is largely nebulous. We also describe a general method for establishing and maintaining semi-continuous cultures of the halophilic microalgal production strain, Dunaliella viridis, that is independent of variations in salinity and illumination intensity. Using this method, the cultures were evaluated to elucidate the overlapping roles of photosynthetic and osmotic adaptation on the accumulation and compositional variation of the biomass, photosynthetic productivity, and physiological biomarkers, as well as spectroscopic and morphological details at the single-cell level. Correlation matrices defining the relationships among the observables and based on variation of the illumination intensity and salinity were constructed for predicting bioproduct yields for varying culture conditions. Following maintenance of stable cultures for 6-week intervals, phenotypic responses to photo-osmotic drift were explored using a combination of single-cell hyperspectral fluorescence imaging and flow cytometry. In addition to morphological changes, release of lipid microparticles from the cells that is disproportionate to cell lysis was observed under hypotonic drift, indicating the existence of a reversible membrane permeation mechanism in Dunaliella. Furthermore, this phenomenon introduces the potential for low-cost strategies for recovering lipids and pigments from the microalgae by minimizing the requirement for energy intensive harvesting and dewatering of the biomass. The results should be applicable to outdoor culture, where seasonal changes resulting in variable solar flux and precipitation and evaporation rates are anticipated.

View Accepted Manuscript (DOE)

Research Organization:: Sandia National Laboratories (SNL-CA), Livermore, CA (United States)

Sponsoring Organization:: USDOE National Nuclear Security Administration (NNSA)

Grant/Contract Number:: AC04-94AL85000

OSTI ID:: 1122967

Report Number(s):: SAND2013--10548J; PII: 331

Journal Information:: Journal of Applied Phycology, Journal Name: Journal of Applied Phycology Journal Issue: 1 Vol. 27; ISSN 0921-8971

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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The role of photo-osmotic adaptation in semi-continuous culture and lipid particle release from Dunaliella viridis

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