Single Molecule and Nanoparticle Imaging in Biophysical, Surface, and Photocatalysis Studies

Thesis/Dissertation · Tue Jan 01 04:00:00 EST 2013

DOI:https://doi.org/10.2172/1116723· OSTI ID:1116723

Ha, Ji Won ^[1]

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1. Iowa State Univ., Ames, IA (United States)

A differential interference contrast (DIC) polarization anisotropy is reported that was successfully used for rotational tracking of gold nanorods attached onto a kinesin-driven microtubule. A dual-wavelength detection of single gold nanorods rotating on a live cell membrane is described. Both transverse and longitudinal surface plasmon resonance (SPR) modes were used for tracking the rotational motions during a fast dynamic process under a DIC microscope. A novel method is presented to determine the full three-dimensional (3D) orientation of single plasmonic gold nanorods rotating on live cell membranes by combining DIC polarization anisotropy with an image pattern recognition technique. Polarization- and wavelength-sensitive DIC microscopy imaging of 2- m long gold nanowires as optical probes in biological studies is reported. A new method is demonstrated to track 3D orientation of single gold nanorods supported on a gold film without angular degeneracy. The idea is to use the interaction (or coupling) of gold nanorods with gold film, yielding characteristic scattering patterns such as a doughnut shape. Imaging of photocatalytic activity, polarity and selectivity on single Au-CdS hybrid nanocatalysts using a high-resolution superlocalization fluorescence imaging technique is described.

• Research Organization: Ames Laboratory (AMES), Ames, IA (United States)
• Sponsoring Organization: USDOE Office of Science (SC)
• DOE Contract Number: AC02-07CH11358
• OSTI ID: 1116723
• Report Number(s): IS--T 3113
• Country of Publication: United States
• Language: English

Related Subjects

36 MATERIALS SCIENCE
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY