Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Selective posttranslational modification of phage-displayed polypeptides

Patent ·
OSTI ID:1108941
; ;
The invention relates to posttranslational modification of phage-displayed polypeptides. These displayed polypeptides comprise at least one unnatural amino acid, e.g., an aryl-azide amino acid such as p-azido-L-phenylalanine, or an alkynyl-amino acid such as para-propargyloxyphenylalanine, which are incorporated into the phage-displayed fusion polypeptide at a selected position by using an in vivo orthogonal translation system comprising a suitable orthogonal aminoacyl-tRNA synthetase and a suitable orthogonal tRNA species. These unnatural amino acids advantageously provide targets for posttranslational modifications such as azide-alkyne [3+2] cycloaddition reactions and Staudinger modifications.
Research Organization:
The Scripps Research Institute, La Jolla, CA, USA
Sponsoring Organization:
USDOE
Assignee:
The Scripps Research Institute (La Jolla, CA)
Patent Number(s):
8,586,340
Application Number:
11/580,223
OSTI ID:
1108941
Country of Publication:
United States
Language:
English

References (38)

Die Erweiterung des genetischen Codes journal January 2005
Expanding the Genetic Code journal January 2005
ChemInform Abstract: 1,3-DIPOLAR CYCLOADDITION - INTRODUCTION, SURVEY, MECHANISM journal May 1985
Über neue organische Phosphorverbindungen III. Phosphinmethylenderivate und Phosphinimine journal January 1919
[15] Biosynthetic method for introducing unnatural amino acids site-specifically into proteins book January 1991
Intermolecular 1,3-Dipolar Cycloadditions book January 1991
Chemoselective ligation reactions with proteins, oligosaccharides and cells journal December 1998
Phage-display technology – finding a needle in a vast molecular haystack journal February 1999
Phage selection for site-specific incorporation of unnatural amino acids into proteins in vivo journal September 2001
Incorporation of non-natural amino acids into proteins journal December 2002
Engineering M13 for phage display journal September 2001
In vivo incorporation of an alkyne into proteins in Escherichia coli journal March 2005
An expanding genetic code journal July 2005
Phage Display journal April 1997
Biosynthetic site-specific incorporation of a non-natural amino acid into a polypeptide journal September 1989
Bioconjugation by Copper(I)-Catalyzed Azide-Alkyne [3 + 2] Cycloaddition journal March 2003
In Vitro Selection of mRNA Display Libraries Containing an Unnatural Amino Acid journal August 2002
Addition of p-Azido-L-phenylalanine to the Genetic Code of Escherichia coli
  • Chin, Jason W.; Santoro, Stephen W.; Martin, Andrew B.
  • Journal of the American Chemical Society, Vol. 124, Issue 31, p. 9026-9027 https://doi.org/10.1021/ja027007w
journal July 2002
A Method for the Generation of Glycoprotein Mimetics journal February 2003
Cell Surface Labeling of Escherichia c oli via Copper(I)-Catalyzed [3+2] Cycloaddition journal September 2003
Adding Amino Acids with Novel Reactivity to the Genetic Code of Saccharomyces Cerevisiae
  • Deiters, Alexander; Cropp, T. Ashton; Mukherji, Mridul
  • Journal of the American Chemical Society, Vol. 125, Issue 39, p. 11782-11783 https://doi.org/10.1021/ja0370037
journal October 2003
A Phage Display System with Unnatural Amino Acids journal December 2004
Phage Display of Selenopeptides journal February 2000
Peptidotriazoles on Solid Phase  [1,2,3]-Triazoles by Regiospecific Copper(I)-Catalyzed 1,3-Dipolar Cycloadditions of Terminal Alkynes to Azides journal May 2002
Phage display: practicalities and prospects journal December 2002
Chemical remodelling of cell surfaces in living animals journal August 2004
An unnatural base pair for incorporating amino acid analogs into proteins journal February 2002
Expanding the genetic code journal December 2001
Incorporation of azides into recombinant proteins for chemoselective modification by the Staudinger ligation journal December 2001
Addition of the keto functional group to the genetic code of Escherichia coli journal December 2002
Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells journal June 2004
An expanded genetic code with a functional quadruplet codon journal May 2004
Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli journal August 2002
Expanding the Genetic Code of Escherichia coli journal April 2001
An Expanded Eukaryotic Genetic Code journal August 2003
A general method for site-specific incorporation of unnatural amino acids into proteins journal April 1989
Cell Surface Engineering by a Modified Staudinger Reaction journal March 2000
Semisynthesis of Proteins by Expressed Protein Ligation journal June 2003

Similar Records

Related Subjects

59 BASIC BIOLOGICAL SCIENCES