Selective posttranslational modification of phage-displayed polypeptides
Patent
·
OSTI ID:1108941
The invention relates to posttranslational modification of phage-displayed polypeptides. These displayed polypeptides comprise at least one unnatural amino acid, e.g., an aryl-azide amino acid such as p-azido-L-phenylalanine, or an alkynyl-amino acid such as para-propargyloxyphenylalanine, which are incorporated into the phage-displayed fusion polypeptide at a selected position by using an in vivo orthogonal translation system comprising a suitable orthogonal aminoacyl-tRNA synthetase and a suitable orthogonal tRNA species. These unnatural amino acids advantageously provide targets for posttranslational modifications such as azide-alkyne [3+2] cycloaddition reactions and Staudinger modifications.
- Research Organization:
- The Scripps Research Institute, La Jolla, CA, USA
- Sponsoring Organization:
- USDOE
- Assignee:
- The Scripps Research Institute (La Jolla, CA)
- Patent Number(s):
- 8,586,340
- Application Number:
- 11/580,223
- OSTI ID:
- 1108941
- Country of Publication:
- United States
- Language:
- English
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