Flow cells for bioanalytical and bioprocess applications with optimized dynamic response and flow characteristics
- Lancaster (Victor R.), San Jose, CA (United States)
- Molecular Devices Corporation, Menlo Park, CA (United States)
In this study, the authors present a method for design and characterization of flow cells developed for minimum flow volume and optimal dynamic response with a given central observation area. The dynamic response of a circular shaped dual ported flow cell was compared to that obtained from a flow cell whose optimized shape was determined using this method. In the optimized flow cell design, the flow rate at the nominal operating pressure increased by 50% whereas the flow cell volume was reduced by 70%. In addition, the dynamic response of the new flow cell was found to be 200% faster than the circular flow cell. The fluid dynamic analysis included simple graphical techniques utilizing free stream vorticity functions and Hagen-Poiseuille relationships. The flow cell dynamic response was measured using a fluorescence detection system. The fluoresce in emission from a 400{micro}m spot located at the exit port was measured as a function of time after switching the input to the flow cell between fluorescent and non-fluorescent solutions. Analysis of results revealed the system could be reasonably characterized as a first order dynamic system. Although some evidence of second order behavior was also observed, it is reasonable to assume that a first order model will provide adequate predictive capability for many real world applications. Given a set of flow cell requirements, the methods presented in this study can be used to design and characterize flow cells with lower reagent consumption and reduced purging times. These improvements can be readily translated into reduced process times and/or lower usage of high cost reagents.
- OSTI ID:
- 110041
- Report Number(s):
- CONF-940659-; ISBN 0-7918-1369-X; TRN: IM9543%%343
- Resource Relation:
- Conference: 1994 American Society of Mechanical Engineers (ASME) Fluids Engineering Division summer meeting, Lake Tahoe, NV (United States), 19-23 Jun 1994; Other Information: PBD: 1994; Related Information: Is Part Of Industrial and environmental applications of fluid mechanics 1994. FED Volume 186; Morrow, T.B.; Horii, K.; Elger, D.F.; Marshall, L.R. [eds.]; PB: 203 p.
- Country of Publication:
- United States
- Language:
- English
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