Facilitating protein solubility by use of peptide extensions

Freimuth, Paul I; Zhang, Yian-Biao; Howitt, Jason

Title: Facilitating protein solubility by use of peptide extensions

Patent · Tue Sep 17 00:00:00 EDT 2013

OSTI ID:1093445

Freimuth, Paul I; Zhang, Yian-Biao; Howitt, Jason

Expression vectors for expression of a protein or polypeptide of interest as a fusion product composed of the protein or polypeptide of interest fused at one terminus to a solubility enhancing peptide extension are provided. Sequences encoding the peptide extensions are provided. The invention further comprises antibodies which bind specifically to one or more of the solubility enhancing peptide extensions.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC02-98CH10886

Assignee:: Brookhaven Science Associates, LLC (Upton, NY)

Patent Number(s):: 8,535,908

Application Number:: 12/684,195

OSTI ID:: 1093445

Country of Publication:: United States

Language:: English

References (8)

Enhanced solubility of recombinant proteins Snavely, Marshall D. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6077689 US Patent Document 6,077,689	patent	June 2000
Rapidly cleavable sumo fusion protein expression system for difficult to express proteins Lima, Christopher D.; Mossessova, Elena https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6872551 US Patent Document 6,872,551	patent	March 2005
Recombinant protein expression in Escherichia coli Baneyx, F. Current Opinion in Biotechnology, Vol. 10, Issue 5 https://doi.org/10.1016/S0958-1669(99)00003-8	journal	October 1999
Fusion protein systems designed to increase soluble cytoplasmic expression of heterologous proteins in Escherichia coli Harrison, Roger G.; Davis, Gregory D. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6207420 US Patent Document 6,207,420	patent	March 2001
Frameshifting in gene 10 of bacteriophage T7 Condron, B. G.; Atkins, J. F.; Gesteland, R. F. Journal of Bacteriology, Vol. 173, Issue 21 https://doi.org/10.1128/jb.173.21.6998-7003.1991	journal	November 1991
Fusion polypeptides and proteins Mascarenhas, Desmond; Zhang, Yang; Olson, Pamela S. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5563046 US Patent Document 5,563,046	patent	October 1996
Fusion tails for the recovery and purification of recombinant proteins Ford, Clark F.; Suominen, Ilari; Glatz, Charles E. Protein Expression and Purification, Vol. 2, Issue 2-3 https://doi.org/10.1016/1046-5928(91)90057-P	journal	April 1991
Fusion protein systems designed to increase soluble cytoplasmic expression of heterologous proteins in esherichia coli Harrison, Roger G.; Davis, Gregory D. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5989868 US Patent Document 5,989,868	patent	November 1999

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