The temporal analysis of yeast exponential phase using shotgun proteomics as a fermentation monitoring technique

Journal Article · Sun Jan 01 00:00:00 EST 2012 · Journal of Proteomics

DOI:https://doi.org/10.1016/j.jprot.2012.06.005· OSTI ID:1091675

Huang, Eric L. ^[1]; Orsat, Valerie ^[2]; Shah, Manesh B ^[3]; Herndon, Elizabeth M ^[3]; Verberkmoes, Nathan C ^[3]; Lefsrud, Mark G ^[1]

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1. McGill University, Montreal, Quebec
2. McGill University
3. ORNL

System biology and bioprocess technology can be better understood using shotgun proteomics as a monitoring system during the fermentation. We demonstrated a shotgun proteomic method to monitor the temporal yeast proteome in early, middle and late exponential phases. Our study identified a total of 1389 proteins combining all 2D-LC-MS/MS runs. The temporal Saccharomyces cerevisiae proteome was enriched with proteolysis, radical detoxification, translation, one-carbon metabolism, glycolysis and TCA cycle. Heat shock proteins and proteins associated with oxidative stress response were found throughout the exponential phase. The most abundant proteins observed were translation elongation factors, ribosomal proteins, chaperones and glycolytic enzymes. The high abundance of the H-protein of the glycine decarboxylase complex (Gcv3p) indicated the availability of glycine in the environment. We observed differentially expressed proteins and the induced proteins at mid-exponential phase were involved in ribosome biogenesis, mitochondria DNA binding/replication and transcriptional activator. Induction of tryptophan synthase (Trp5p) indicated the abundance of tryptophan during the fermentation. As fermentation progressed toward late exponential phase, a decrease in cell proliferation was implied from the repression of ribosomal proteins, transcription coactivators, methionine aminopeptidase and translation-associated proteins.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: USDOE Office of Science (SC)
• DOE Contract Number: DE-AC05-00OR22725
• OSTI ID: 1091675
• Journal Information: Journal of Proteomics, Vol. 75, Issue 17; ISSN 1874-3919
• Country of Publication: United States
• Language: English