Comparative c-type cytochrome expression analysis in Shewanella oneidensis strain MR-1 and Anaeromyxobacter dehalogenans strain 2CP-C grown with soluble and insoluble oxidised metal electron acceptors

Journal Article · Sun Jan 01 04:00:00 EST 2012 · Biochemical Society Transactions

DOI:https://doi.org/10.1042/BST20120182· OSTI ID:1088116

Nissen, Silke ^[1]; Liu, Xiaoxin ^[1]; Chourey, Karuna ^[1]; Wagner, Darlene D ^[2]; Pffifner, Susan ^[3]; Loeffler, Frank E ^[1]

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1. ORNL
2. Georgia Institute of Technology
3. University of Tennessee, Knoxville (UTK)

The genomes of Shewanella oneidensis strain MR-1 and Anaeromyxobacter dehalogenans strain 2CP-C encode 40 and 69 putative c-type cytochrome genes, respectively. Deletion mutant and biochemical studies have assigned specific functions to a few c-type cytochromes involved in electron transfer to oxidised metals in Shewanella oneidensis strain MR-1. Although promising, the genetic approach is limited to gene deletions that produce a distinct phenotype, and organism for which a genetic system is available. To more comprehensively investigate and compare c-type cytochrome expression in Shewanella oneidensis strain MR-1 and Anaeromyxobacter dehalogenans strain 2CP-C, proteomic measurements were used to characterise lysates of cells grown with soluble Fe(III) (as ferric citrate) and insoluble Mn(IV) (as MnO2) as electron acceptors. Strain MR-1 expressed 19 and 20, and strain 2CP-C expressed 27 and 25 c-type cytochromes when grown with Fe(III) and Mn(IV), respectively. The majority of c-type cytochromes (77% for strain MR-1 and 63% for strain 2CP-C) were expressed under both growth conditions; however, the analysis also revealed unique c-type cytochromes that were specifically expressed in cells grown with soluble Fe(III) or insoluble Mn(IV). Proteomic characterisation proved to be a promising approach for determining the c-type cytochrome complement expressed under different growth conditions, and will help elucidating the specific functions of more c-type cytochromes that are the basis for Shewanella and Anaeromyxobacter respiratory versatility.

• Research Organization: Oak Ridge National Laboratory (ORNL)
• Sponsoring Organization: SC USDOE - Office of Science (SC)
• DOE Contract Number: AC05-00OR22725
• OSTI ID: 1088116
• Journal Information: Biochemical Society Transactions, Journal Name: Biochemical Society Transactions Journal Issue: 6 Vol. 40; ISSN 0300-5127
• Country of Publication: United States
• Language: English

Related Subjects

Anaeromyxobacter dehalogenans strain 2CP-C
Proteomics
Shewanella oneidensis strain MR-1
c-type cytochromes
metal respiration