Mechanistic and Quantitative Studies of Bystander Response in 3D Tissues for Low-Dose Radiation Risk Estimations

Technical Report · Fri Apr 04 00:00:00 EDT 2014

OSTI ID:1083233

Amundson, Sally A. ^[1]

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1. Columbia Univ., New York, NY (United States); The Trustees of Columbia University

We have used the MatTek 3-dimensional human skin model to study the gene expression response of a 3D model to low and high dose low LET radiation, and to study the radiation bystander effect as a function of distance from the site of irradiation with either alpha particles or low LET protons. We have found response pathways that appear to be specific for low dose exposures, that could not have been predicted from high dose studies. We also report the time and distance dependent expression of a large number of genes in bystander tissue. the bystander response in 3D tissues showed many similarities to that described previously in 2D cultured cells, but also showed some differences.

• Research Organization: Columbia Univ., New York, NY (United States)
• Sponsoring Organization: USDOE Office of Science (SC)
• DOE Contract Number: FG02-07ER64336
• OSTI ID: 1083233
• Report Number(s): DOE-ER--64336-3
• Country of Publication: United States
• Language: English

Related Subjects

3D Tissue Model
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
Bystander Effects
Gene Expression
Ionizing Radiation